The effect of Euonymus Fortunei extract combined with oxaliplatin on the IKKβ/NF-κB signaling pathway in colon cancer
[Objectives] To investigate the effects of Euonymus Fortunei extract combined with oxaliplatin on tumor growth and the expression of inflammatory cytokines in colon cancer. [Methods] BALB/c nude mice were subcutaneously injected with CT26 colon cancer cells to establish a xenograft model of colon ca...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Colorectal & Anal Surgery
2024-10-01
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| Series: | 结直肠肛门外科 |
| Subjects: | |
| Online Access: | https://jcas.gxmuyfy.cn/cn/wqll/paper.html?id=361&cateName=2024%E5%B9%B4%20%E7%AC%AC30%E5%8D%B7%20%E7%AC%AC5%E6%9C%9F |
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| Summary: | [Objectives] To investigate the effects of Euonymus Fortunei extract combined with oxaliplatin on tumor growth and the expression of inflammatory cytokines in colon cancer. [Methods] BALB/c nude mice were subcutaneously injected with CT26 colon cancer cells to establish a xenograft model of colon cancer. Thirty-two nude mice were randomly divided into four groups: the tumor-bearing group, the oxaliplatin intervention group, the Euonymus Fortunei intervention group, and the combined intervention group (Euonymus Fortunei combined with oxaliplatin), with 8 mice in each group. The interventions were continuously administered for 3 weeks. Tumor volume, tumor mass were measured, and the tumor inhibition rate was calculated. The levels of IL-1, IL-6, and TNF-α in serum were measured using ELISA. The expression of IKKβ and NF-κB proteins in tumor tissues was detected by Western blotting. [Results] Compared with the tumor-bearing group, the Euonymus Fortunei intervention group showed no significant difference in tumor volume and tumor mass (P>0.05), but the levels of IL-1, IL-6, and TNF-α in serum and the relative expression of IKKβ and NF-κB proteins in tumor tissues were significantly reduced (P<0.05). Compared with the oxaliplatin intervention group, the combined intervention group showed significantly reduced tumor volume and tumor mass (P<0.05), and a significantly increased tumor inhibition rate (69.26%). The levels of IL-1, IL-6, and TNF-α in serum and the relative expression of IKKβ and NF-κB proteins in tumor tissues were significantly reduced (P<0.05). [Conclusion] Although monotherapy with Euonymus Fortunei did not significantly inhibit tumor growth in colon cancer, its combination with oxaliplatin showed a significant inhibitory effect on tumor growth. This effect may be related to the regulation of the IKKβ/NF-κB signaling pathway by Euonymus Fortunei extract. |
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| ISSN: | 1674-0491 |