Phenotypic and molecular identification of extended-spectrum beta-lactamases in escherichia coli in healthy broilers at the west region of iran
<b>Aim:</b> The presence of fecal carriers of extended-spectrum beta- lactamase (ESBL) genes in poultry is worrisome because it is likely to spread to a human being. This study was proposed to estimate the frequency of ESBLs carriers in Escherichia coli isolated from healthy broilers gut...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1217 |
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| Summary: | <b>Aim:</b> The presence of fecal carriers of extended-spectrum beta-
lactamase (ESBL) genes in poultry is worrisome because it
is likely to spread to a human being. This study was proposed
to estimate the frequency of ESBLs carriers in Escherichia coli
isolated from healthy broilers gut in west of Iran.<p>
<b>Materials and Methods:</b> A total of 300 fecal samples were collected
from apparently healthy broiler. E. coli strains were isolated,
confirmed molecularly and studied using disk combination
method, antimicrobial susceptibility test and ESBL-detection
polymerase chain reactions (PCR).<p>
<b>Results:</b> Two hundred and eighty five strains of E. coli were
isolated biochemically and all were confirmed in a speciesspecific
PCR reaction. Antimicrobial susceptibility test on 156
ESBL-producing E. coli identified by phenotypic method revealed
that 100 % of them were resistant to at least one antibiotic,
while all were sensitive to imipenem. The prevalence of blaTEM,
blaCTX-M, and blaSHV genes was 52.63%, 38.59%, and 30.17%
respectively. The simultaneous existence of several ESBL genes
was identified in 52.61% of the isolates.<p>
<b>Conclusions:</b> The vast prevalence of ESBL carriers with multiple
resistance in poultry is a threat to public health. Consistent
monitoring and proper use of antibiotics in the regions poultry
industry should be applied. |
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| ISSN: | 1309-6958 2146-1953 |