Characteristics prediction of insect chitin synthase

According to the chitin synthase (CS) gene sequences deposited in GenBank, characteristics of insect CS were studied by software solution including GenDoc, PROSITE Motif search and PredictProtein. Eleven conserved domains at the amino acid sequence level, such as CAT (M/L) WHEX<sub>3</sub&g...

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Main Authors: LU Jian-liang, LIN Chen, YANG Xiao-li, ZHENG Xin-qiang, LIANG Yue-rong
Format: Article
Language:English
Published: Zhejiang University Press 2008-09-01
Series:浙江大学学报. 农业与生命科学版
Subjects:
Online Access:https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.05.003
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author LU Jian-liang
LIN Chen
YANG Xiao-li
ZHENG Xin-qiang
LIANG Yue-rong
author_facet LU Jian-liang
LIN Chen
YANG Xiao-li
ZHENG Xin-qiang
LIANG Yue-rong
author_sort LU Jian-liang
collection DOAJ
description According to the chitin synthase (CS) gene sequences deposited in GenBank, characteristics of insect CS were studied by software solution including GenDoc, PROSITE Motif search and PredictProtein. Eleven conserved domains at the amino acid sequence level, such as CAT (M/L) WHEX<sub>3</sub> EMX<sub>3</sub> LKSI, were found in insect CS. Ten of them were predicted in catalysis center which located inside of membrane and faced to cytoplast, the other one belonged to a coiled-coil region which located outside of membrane. Secondary structures were predicted that insect CS had high ratio of α helix and a compact shape. Additional transmembrane helixes were found in the C-terminal and N-terminal of insect CS comparing with the fungus. These helixes might be responsible for the transportation and localization of insect CS. It was also presumed that the coiled-coil region was the key domain for formation of CS high level structures and package of the new chitin strands.
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series 浙江大学学报. 农业与生命科学版
spelling doaj-art-5b25c7a6db5f4c70a64869c015ffc77b2025-08-20T03:16:13ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552008-09-013449150110.3785/j.issn.1008-9209.2008.05.00310089209Characteristics prediction of insect chitin synthaseLU Jian-liangLIN ChenYANG Xiao-liZHENG Xin-qiangLIANG Yue-rongAccording to the chitin synthase (CS) gene sequences deposited in GenBank, characteristics of insect CS were studied by software solution including GenDoc, PROSITE Motif search and PredictProtein. Eleven conserved domains at the amino acid sequence level, such as CAT (M/L) WHEX<sub>3</sub> EMX<sub>3</sub> LKSI, were found in insect CS. Ten of them were predicted in catalysis center which located inside of membrane and faced to cytoplast, the other one belonged to a coiled-coil region which located outside of membrane. Secondary structures were predicted that insect CS had high ratio of α helix and a compact shape. Additional transmembrane helixes were found in the C-terminal and N-terminal of insect CS comparing with the fungus. These helixes might be responsible for the transportation and localization of insect CS. It was also presumed that the coiled-coil region was the key domain for formation of CS high level structures and package of the new chitin strands.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.05.003chitin synthaseconserved domains and special motifssecondary structureinsect
spellingShingle LU Jian-liang
LIN Chen
YANG Xiao-li
ZHENG Xin-qiang
LIANG Yue-rong
Characteristics prediction of insect chitin synthase
浙江大学学报. 农业与生命科学版
chitin synthase
conserved domains and special motifs
secondary structure
insect
title Characteristics prediction of insect chitin synthase
title_full Characteristics prediction of insect chitin synthase
title_fullStr Characteristics prediction of insect chitin synthase
title_full_unstemmed Characteristics prediction of insect chitin synthase
title_short Characteristics prediction of insect chitin synthase
title_sort characteristics prediction of insect chitin synthase
topic chitin synthase
conserved domains and special motifs
secondary structure
insect
url https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.05.003
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AT yangxiaoli characteristicspredictionofinsectchitinsynthase
AT zhengxinqiang characteristicspredictionofinsectchitinsynthase
AT liangyuerong characteristicspredictionofinsectchitinsynthase