Action of vitamin D and the receptor, VDRa, in calcium handling in zebrafish (Danio rerio).
The purpose of the present study was to use zebrafish as a model to investigate how vitamin D and its receptors interact to control Ca(2+) uptake function. Low-Ca(2+) fresh water stimulated Ca(2+) influx and expressions of epithelial calcium channel (ecac), vitamin D-25-hydroxylase (cyp2r1), vitamin...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0045650&type=printable |
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| author | Chia-Hao Lin Che-Hsien Su Deng-Yu Tseng Feng-Chun Ding Pung-Pung Hwang |
| author_facet | Chia-Hao Lin Che-Hsien Su Deng-Yu Tseng Feng-Chun Ding Pung-Pung Hwang |
| author_sort | Chia-Hao Lin |
| collection | DOAJ |
| description | The purpose of the present study was to use zebrafish as a model to investigate how vitamin D and its receptors interact to control Ca(2+) uptake function. Low-Ca(2+) fresh water stimulated Ca(2+) influx and expressions of epithelial calcium channel (ecac), vitamin D-25-hydroxylase (cyp2r1), vitamin D receptor a (vdra), and vdrb in zebrafish. Exogenous vitamin D increased Ca(2+) influx and expressions of ecac and 25-hydroxyvitamin D(3)-24-hydroxylase (cyp24a1), but downregulated 1α-OHase (cyp27b1) with no effects on other Ca(2+) transporters. Morpholino oligonucleotide knockdown of VDRa, but not VDRb, was found as a consequence of calcium uptake inhibition by knockdown of ecac, and ossification of vertebrae is impaired. Taken together, vitamin D-VDRa signaling may stimulate Ca(2+) uptake by upregulating ECaC in zebrafish, thereby clarifying the Ca(2+)-handling function of only a VDR in teleosts. Zebrafish may be useful as a model to explore the function of vitamin D-VDR signaling in Ca(2+) homeostasis and the related physiological processes in vertebrates. |
| format | Article |
| id | doaj-art-5b1e9313b79f4b81b7a7880ec5ae35db |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-5b1e9313b79f4b81b7a7880ec5ae35db2025-08-20T03:00:50ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0179e4565010.1371/journal.pone.0045650Action of vitamin D and the receptor, VDRa, in calcium handling in zebrafish (Danio rerio).Chia-Hao LinChe-Hsien SuDeng-Yu TsengFeng-Chun DingPung-Pung HwangThe purpose of the present study was to use zebrafish as a model to investigate how vitamin D and its receptors interact to control Ca(2+) uptake function. Low-Ca(2+) fresh water stimulated Ca(2+) influx and expressions of epithelial calcium channel (ecac), vitamin D-25-hydroxylase (cyp2r1), vitamin D receptor a (vdra), and vdrb in zebrafish. Exogenous vitamin D increased Ca(2+) influx and expressions of ecac and 25-hydroxyvitamin D(3)-24-hydroxylase (cyp24a1), but downregulated 1α-OHase (cyp27b1) with no effects on other Ca(2+) transporters. Morpholino oligonucleotide knockdown of VDRa, but not VDRb, was found as a consequence of calcium uptake inhibition by knockdown of ecac, and ossification of vertebrae is impaired. Taken together, vitamin D-VDRa signaling may stimulate Ca(2+) uptake by upregulating ECaC in zebrafish, thereby clarifying the Ca(2+)-handling function of only a VDR in teleosts. Zebrafish may be useful as a model to explore the function of vitamin D-VDR signaling in Ca(2+) homeostasis and the related physiological processes in vertebrates.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0045650&type=printable |
| spellingShingle | Chia-Hao Lin Che-Hsien Su Deng-Yu Tseng Feng-Chun Ding Pung-Pung Hwang Action of vitamin D and the receptor, VDRa, in calcium handling in zebrafish (Danio rerio). PLoS ONE |
| title | Action of vitamin D and the receptor, VDRa, in calcium handling in zebrafish (Danio rerio). |
| title_full | Action of vitamin D and the receptor, VDRa, in calcium handling in zebrafish (Danio rerio). |
| title_fullStr | Action of vitamin D and the receptor, VDRa, in calcium handling in zebrafish (Danio rerio). |
| title_full_unstemmed | Action of vitamin D and the receptor, VDRa, in calcium handling in zebrafish (Danio rerio). |
| title_short | Action of vitamin D and the receptor, VDRa, in calcium handling in zebrafish (Danio rerio). |
| title_sort | action of vitamin d and the receptor vdra in calcium handling in zebrafish danio rerio |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0045650&type=printable |
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