Detection of DNA Viruses in Free-Ranging Rat Populations in Hungary
To address a gap in our understanding of viral infections in epidemiologically important rat species, we aimed to detect DNA viruses from the tissues of free-ranging rat populations in Hungary. DNA viruses were identified from the parenchymal organs of 230 <i>Rattus norvegicus</i> and &l...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/16/12/1948 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850037275270840320 |
|---|---|
| author | Márton Z. Vidovszky András Surján Gábor Földvári László Egyed |
| author_facet | Márton Z. Vidovszky András Surján Gábor Földvári László Egyed |
| author_sort | Márton Z. Vidovszky |
| collection | DOAJ |
| description | To address a gap in our understanding of viral infections in epidemiologically important rat species, we aimed to detect DNA viruses from the tissues of free-ranging rat populations in Hungary. DNA viruses were identified from the parenchymal organs of 230 <i>Rattus norvegicus</i> and <i>Rattus rattus</i>, using family-specific pan-PCR assays followed by sequencing of the PCR products. Adeno-, herpes-, circo-, and polyomaviruses were detected, while irido-, pox-, and dependoparvoviruses were not. Adenovirus DNA was present in 6.5% of the samples, herpesvirus and polyomavirus DNA in 12.2%, and circovirus DNA in 1.7%. All detected herpesviruses belonged to the β and γ subfamilies, with a majority being β herpesviruses. Some adenovirus and herpesvirus sequences were novel, while only the known Rattus norvegicus polyomavirus 1 was detected for polyomaviruses. The rare circovirus-positive samples revealed the presence of both rodent and bird circoviruses, indicating the ability of circoviruses to cross species barriers. Our findings show that rats host a variety of DNA viruses, many of which were previously uncharacterized, highlighting the need for further diagnostic studies. |
| format | Article |
| id | doaj-art-5b18cbcea73442aba15fa12ac3606cde |
| institution | DOAJ |
| issn | 1999-4915 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-5b18cbcea73442aba15fa12ac3606cde2025-08-20T02:56:55ZengMDPI AGViruses1999-49152024-12-011612194810.3390/v16121948Detection of DNA Viruses in Free-Ranging Rat Populations in HungaryMárton Z. Vidovszky0András Surján1Gábor Földvári2László Egyed3HUN-REN Veterinary Medical Research Institute, 1143 Budapest, HungaryHUN-REN Veterinary Medical Research Institute, 1143 Budapest, HungaryInstitute of Evolution, HUN-REN Centre for Ecological Research, 1121 Budapest, HungaryHUN-REN Veterinary Medical Research Institute, 1143 Budapest, HungaryTo address a gap in our understanding of viral infections in epidemiologically important rat species, we aimed to detect DNA viruses from the tissues of free-ranging rat populations in Hungary. DNA viruses were identified from the parenchymal organs of 230 <i>Rattus norvegicus</i> and <i>Rattus rattus</i>, using family-specific pan-PCR assays followed by sequencing of the PCR products. Adeno-, herpes-, circo-, and polyomaviruses were detected, while irido-, pox-, and dependoparvoviruses were not. Adenovirus DNA was present in 6.5% of the samples, herpesvirus and polyomavirus DNA in 12.2%, and circovirus DNA in 1.7%. All detected herpesviruses belonged to the β and γ subfamilies, with a majority being β herpesviruses. Some adenovirus and herpesvirus sequences were novel, while only the known Rattus norvegicus polyomavirus 1 was detected for polyomaviruses. The rare circovirus-positive samples revealed the presence of both rodent and bird circoviruses, indicating the ability of circoviruses to cross species barriers. Our findings show that rats host a variety of DNA viruses, many of which were previously uncharacterized, highlighting the need for further diagnostic studies.https://www.mdpi.com/1999-4915/16/12/1948free-ranging <i>Rattus norvegicus</i><i>Rattus rattus</i>PCRadenovirusesherpesvirusespolyomaviruses |
| spellingShingle | Márton Z. Vidovszky András Surján Gábor Földvári László Egyed Detection of DNA Viruses in Free-Ranging Rat Populations in Hungary Viruses free-ranging <i>Rattus norvegicus</i> <i>Rattus rattus</i> PCR adenoviruses herpesviruses polyomaviruses |
| title | Detection of DNA Viruses in Free-Ranging Rat Populations in Hungary |
| title_full | Detection of DNA Viruses in Free-Ranging Rat Populations in Hungary |
| title_fullStr | Detection of DNA Viruses in Free-Ranging Rat Populations in Hungary |
| title_full_unstemmed | Detection of DNA Viruses in Free-Ranging Rat Populations in Hungary |
| title_short | Detection of DNA Viruses in Free-Ranging Rat Populations in Hungary |
| title_sort | detection of dna viruses in free ranging rat populations in hungary |
| topic | free-ranging <i>Rattus norvegicus</i> <i>Rattus rattus</i> PCR adenoviruses herpesviruses polyomaviruses |
| url | https://www.mdpi.com/1999-4915/16/12/1948 |
| work_keys_str_mv | AT martonzvidovszky detectionofdnavirusesinfreerangingratpopulationsinhungary AT andrassurjan detectionofdnavirusesinfreerangingratpopulationsinhungary AT gaborfoldvari detectionofdnavirusesinfreerangingratpopulationsinhungary AT laszloegyed detectionofdnavirusesinfreerangingratpopulationsinhungary |