Regulatory variation controlling architectural pleiotropy in maize

Abstract An early event in plant organogenesis is establishment of a boundary between the stem cell containing meristem and differentiating lateral organ. In maize (Zea mays), evidence suggests a common gene network functions at boundaries of distinct organs and contributes to pleiotropy between lea...

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Main Authors: Edoardo Bertolini, Brian R. Rice, Max Braud, Jiani Yang, Sarah Hake, Josh Strable, Alexander E. Lipka, Andrea L. Eveland
Format: Article
Language:English
Published: Nature Portfolio 2025-03-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-56884-w
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author Edoardo Bertolini
Brian R. Rice
Max Braud
Jiani Yang
Sarah Hake
Josh Strable
Alexander E. Lipka
Andrea L. Eveland
author_facet Edoardo Bertolini
Brian R. Rice
Max Braud
Jiani Yang
Sarah Hake
Josh Strable
Alexander E. Lipka
Andrea L. Eveland
author_sort Edoardo Bertolini
collection DOAJ
description Abstract An early event in plant organogenesis is establishment of a boundary between the stem cell containing meristem and differentiating lateral organ. In maize (Zea mays), evidence suggests a common gene network functions at boundaries of distinct organs and contributes to pleiotropy between leaf angle and tassel branch number, two agronomic traits. To uncover regulatory variation at the nexus of these two traits, we use regulatory network topologies derived from specific developmental contexts to guide multivariate genome-wide association analyses. In addition to defining network plasticity around core pleiotropic loci, we identify new transcription factors that contribute to phenotypic variation in canopy architecture, and structural variation that contributes to cis-regulatory control of pleiotropy between tassel branching and leaf angle across maize diversity. Results demonstrate the power of informing statistical genetics with context-specific developmental networks to pinpoint pleiotropic loci and their cis-regulatory components, which can be used to fine-tune plant architecture for crop improvement.
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spelling doaj-art-5b08d16c90e446e0abd1cd2b6b4ed7032025-08-20T02:59:54ZengNature PortfolioNature Communications2041-17232025-03-0116111810.1038/s41467-025-56884-wRegulatory variation controlling architectural pleiotropy in maizeEdoardo Bertolini0Brian R. Rice1Max Braud2Jiani Yang3Sarah Hake4Josh Strable5Alexander E. Lipka6Andrea L. Eveland7Donald Danforth Plant Science CenterDepartment of Crop Sciences, University of Illinois, Urbana-Donald Danforth Plant Science CenterDonald Danforth Plant Science CenterPlant Gene Expression Center, USDA-ARSDepartment of Molecular and Structural Biochemistry, North Carolina State UniversityDepartment of Crop Sciences, University of Illinois, Urbana-Donald Danforth Plant Science CenterAbstract An early event in plant organogenesis is establishment of a boundary between the stem cell containing meristem and differentiating lateral organ. In maize (Zea mays), evidence suggests a common gene network functions at boundaries of distinct organs and contributes to pleiotropy between leaf angle and tassel branch number, two agronomic traits. To uncover regulatory variation at the nexus of these two traits, we use regulatory network topologies derived from specific developmental contexts to guide multivariate genome-wide association analyses. In addition to defining network plasticity around core pleiotropic loci, we identify new transcription factors that contribute to phenotypic variation in canopy architecture, and structural variation that contributes to cis-regulatory control of pleiotropy between tassel branching and leaf angle across maize diversity. Results demonstrate the power of informing statistical genetics with context-specific developmental networks to pinpoint pleiotropic loci and their cis-regulatory components, which can be used to fine-tune plant architecture for crop improvement.https://doi.org/10.1038/s41467-025-56884-w
spellingShingle Edoardo Bertolini
Brian R. Rice
Max Braud
Jiani Yang
Sarah Hake
Josh Strable
Alexander E. Lipka
Andrea L. Eveland
Regulatory variation controlling architectural pleiotropy in maize
Nature Communications
title Regulatory variation controlling architectural pleiotropy in maize
title_full Regulatory variation controlling architectural pleiotropy in maize
title_fullStr Regulatory variation controlling architectural pleiotropy in maize
title_full_unstemmed Regulatory variation controlling architectural pleiotropy in maize
title_short Regulatory variation controlling architectural pleiotropy in maize
title_sort regulatory variation controlling architectural pleiotropy in maize
url https://doi.org/10.1038/s41467-025-56884-w
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