Flow Cytometric Test with Eosin-5-Maleimide for a Diagnosis of Hereditary Spherocytosis in a Newborn
A term male newborn born to a mother who had hereditary spherocytosis presented with neonatal jaundice at 20 hours of life. Complete blood count showed hemoglobin 17.1 g/dL, MCV 104.2 fL, MCH 32.9 pg, and MCHC 31.6 g/dL. The patient had indirect hyperbilirubinemia requiring phototherapy. The maximum...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Case Reports in Hematology |
| Online Access: | http://dx.doi.org/10.1155/2019/5925731 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849411625171288064 |
|---|---|
| author | Kanda Fanhchaksai Suphara Manowong Rungrote Natesirinilkul Lalita Sathitsamitphong Pimlak Charoenkwan |
| author_facet | Kanda Fanhchaksai Suphara Manowong Rungrote Natesirinilkul Lalita Sathitsamitphong Pimlak Charoenkwan |
| author_sort | Kanda Fanhchaksai |
| collection | DOAJ |
| description | A term male newborn born to a mother who had hereditary spherocytosis presented with neonatal jaundice at 20 hours of life. Complete blood count showed hemoglobin 17.1 g/dL, MCV 104.2 fL, MCH 32.9 pg, and MCHC 31.6 g/dL. The patient had indirect hyperbilirubinemia requiring phototherapy. The maximum total bilirubin level was 12.15 mg/dL at 20 hours of life. Peripheral blood smear revealed spherocytes, crenated red cells, and polychromasia. A flow cytometric test with eosin-5-maleimide- (EMA-) labeled RBC was performed in the patient and parents. The fluorescence histograms of EMA-labeled RBC from the patient and mother were shifted to the left, and the fluorescence ratio when compared with normal was 0.69 and 0.84, respectively. The flow cytometric test with EMA is useful in supporting the diagnosis of hereditary spherocytosis during newborn period. |
| format | Article |
| id | doaj-art-5b082b143a3043b4be962b870e542db3 |
| institution | Kabale University |
| issn | 2090-6560 2090-6579 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Case Reports in Hematology |
| spelling | doaj-art-5b082b143a3043b4be962b870e542db32025-08-20T03:34:43ZengWileyCase Reports in Hematology2090-65602090-65792019-01-01201910.1155/2019/59257315925731Flow Cytometric Test with Eosin-5-Maleimide for a Diagnosis of Hereditary Spherocytosis in a NewbornKanda Fanhchaksai0Suphara Manowong1Rungrote Natesirinilkul2Lalita Sathitsamitphong3Pimlak Charoenkwan4Research Cluster of Thalassemia and Red Blood Cell Disorders, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Pediatrics, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandA term male newborn born to a mother who had hereditary spherocytosis presented with neonatal jaundice at 20 hours of life. Complete blood count showed hemoglobin 17.1 g/dL, MCV 104.2 fL, MCH 32.9 pg, and MCHC 31.6 g/dL. The patient had indirect hyperbilirubinemia requiring phototherapy. The maximum total bilirubin level was 12.15 mg/dL at 20 hours of life. Peripheral blood smear revealed spherocytes, crenated red cells, and polychromasia. A flow cytometric test with eosin-5-maleimide- (EMA-) labeled RBC was performed in the patient and parents. The fluorescence histograms of EMA-labeled RBC from the patient and mother were shifted to the left, and the fluorescence ratio when compared with normal was 0.69 and 0.84, respectively. The flow cytometric test with EMA is useful in supporting the diagnosis of hereditary spherocytosis during newborn period.http://dx.doi.org/10.1155/2019/5925731 |
| spellingShingle | Kanda Fanhchaksai Suphara Manowong Rungrote Natesirinilkul Lalita Sathitsamitphong Pimlak Charoenkwan Flow Cytometric Test with Eosin-5-Maleimide for a Diagnosis of Hereditary Spherocytosis in a Newborn Case Reports in Hematology |
| title | Flow Cytometric Test with Eosin-5-Maleimide for a Diagnosis of Hereditary Spherocytosis in a Newborn |
| title_full | Flow Cytometric Test with Eosin-5-Maleimide for a Diagnosis of Hereditary Spherocytosis in a Newborn |
| title_fullStr | Flow Cytometric Test with Eosin-5-Maleimide for a Diagnosis of Hereditary Spherocytosis in a Newborn |
| title_full_unstemmed | Flow Cytometric Test with Eosin-5-Maleimide for a Diagnosis of Hereditary Spherocytosis in a Newborn |
| title_short | Flow Cytometric Test with Eosin-5-Maleimide for a Diagnosis of Hereditary Spherocytosis in a Newborn |
| title_sort | flow cytometric test with eosin 5 maleimide for a diagnosis of hereditary spherocytosis in a newborn |
| url | http://dx.doi.org/10.1155/2019/5925731 |
| work_keys_str_mv | AT kandafanhchaksai flowcytometrictestwitheosin5maleimideforadiagnosisofhereditaryspherocytosisinanewborn AT supharamanowong flowcytometrictestwitheosin5maleimideforadiagnosisofhereditaryspherocytosisinanewborn AT rungrotenatesirinilkul flowcytometrictestwitheosin5maleimideforadiagnosisofhereditaryspherocytosisinanewborn AT lalitasathitsamitphong flowcytometrictestwitheosin5maleimideforadiagnosisofhereditaryspherocytosisinanewborn AT pimlakcharoenkwan flowcytometrictestwitheosin5maleimideforadiagnosisofhereditaryspherocytosisinanewborn |