Production and characterization of sophorolipid under yeast-mediated submerged fermentation utilizing Agro-industrial waste
The challenges of pollution and agro-industrial waste management have led to the development of bioconversion techniques to transform these wastes into valuable products. This has increased the focus on the sustainable and cost-efficient production of biosurfactants from agro-industrial waste. Hence...
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Elsevier
2025-01-01
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| Series: | Current Research in Microbial Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666517424001172 |
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| author | Aishwary Purohit Amar Jyoti Das Debashish Ghosh |
| author_facet | Aishwary Purohit Amar Jyoti Das Debashish Ghosh |
| author_sort | Aishwary Purohit |
| collection | DOAJ |
| description | The challenges of pollution and agro-industrial waste management have led to the development of bioconversion techniques to transform these wastes into valuable products. This has increased the focus on the sustainable and cost-efficient production of biosurfactants from agro-industrial waste. Hence, the present study investigates the production of sophorolipid biosurfactants using the yeast strain Rhodotorula mucilaginosa IIPL32 under submerged fermentation, employing sugarcane bagasse hydrolysate—a renewable, low-cost agro-industrial waste as the feedstock. By systematically optimizing strain adaptation, medium composition, and scaling up the process from shake flasks to a bioreactor, a maximum sophorolipid yield of 2.6 ± 0.21 g/L was achieved. Extensive characterization was conducted, encompassing emulsification index (54 %), surface tension reduction, and several chemical analyses (anthrone, iodine, saponification, lipid solubility). Advanced structural elucidation techniques such as Fourier-transform infrared (FTIR) spectroscopy, liquid chromatography-mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR) spectroscopy were employed for structural confirmation of the extracted biosurfactant. FTIR spectroscopy identified characteristic functional groups, while LC-MS revealed distinct sophorolipid congeners with varying lipid chain lengths and acetylation. NMR spectroscopy corroborated the presence of disaccharide and fatty acid components, indicating the extracted biosurfactant might be sophorolipid. This study underscores the feasibility of utilizing agro-industrial waste for the eco-friendly production of sophorolipid biosurfactants and provides detailed insights into their structural features, highlighting their potential applications across diverse fields such as pharmaceuticals, cosmetics, and environmental remediation. |
| format | Article |
| id | doaj-art-5b04f3890cc54ed8ab0a63d3e867c09c |
| institution | Kabale University |
| issn | 2666-5174 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Microbial Sciences |
| spelling | doaj-art-5b04f3890cc54ed8ab0a63d3e867c09c2024-12-30T04:16:01ZengElsevierCurrent Research in Microbial Sciences2666-51742025-01-018100334Production and characterization of sophorolipid under yeast-mediated submerged fermentation utilizing Agro-industrial wasteAishwary Purohit0Amar Jyoti Das1Debashish Ghosh2Department of Microbiology, Graphic era Deemed to be University, Dehradun 248002, Uttarakhand, India; Material Resource Efficiency Division, CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun 248005, Uttarakhand, IndiaDepartment of Microbiology, Graphic era Deemed to be University, Dehradun 248002, Uttarakhand, India; Corresponding author: Department of Microbiology, Graphic Era Deemed to be University, Dehradun, Uttarakhand 248002, India.Material Resource Efficiency Division, CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun 248005, Uttarakhand, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201 002, Uttar Pradesh, IndiaThe challenges of pollution and agro-industrial waste management have led to the development of bioconversion techniques to transform these wastes into valuable products. This has increased the focus on the sustainable and cost-efficient production of biosurfactants from agro-industrial waste. Hence, the present study investigates the production of sophorolipid biosurfactants using the yeast strain Rhodotorula mucilaginosa IIPL32 under submerged fermentation, employing sugarcane bagasse hydrolysate—a renewable, low-cost agro-industrial waste as the feedstock. By systematically optimizing strain adaptation, medium composition, and scaling up the process from shake flasks to a bioreactor, a maximum sophorolipid yield of 2.6 ± 0.21 g/L was achieved. Extensive characterization was conducted, encompassing emulsification index (54 %), surface tension reduction, and several chemical analyses (anthrone, iodine, saponification, lipid solubility). Advanced structural elucidation techniques such as Fourier-transform infrared (FTIR) spectroscopy, liquid chromatography-mass spectrometry (LC-MS), and nuclear magnetic resonance (NMR) spectroscopy were employed for structural confirmation of the extracted biosurfactant. FTIR spectroscopy identified characteristic functional groups, while LC-MS revealed distinct sophorolipid congeners with varying lipid chain lengths and acetylation. NMR spectroscopy corroborated the presence of disaccharide and fatty acid components, indicating the extracted biosurfactant might be sophorolipid. This study underscores the feasibility of utilizing agro-industrial waste for the eco-friendly production of sophorolipid biosurfactants and provides detailed insights into their structural features, highlighting their potential applications across diverse fields such as pharmaceuticals, cosmetics, and environmental remediation.http://www.sciencedirect.com/science/article/pii/S2666517424001172Agro-industrial wasteBagasseSophorolipidRenewable resource |
| spellingShingle | Aishwary Purohit Amar Jyoti Das Debashish Ghosh Production and characterization of sophorolipid under yeast-mediated submerged fermentation utilizing Agro-industrial waste Current Research in Microbial Sciences Agro-industrial waste Bagasse Sophorolipid Renewable resource |
| title | Production and characterization of sophorolipid under yeast-mediated submerged fermentation utilizing Agro-industrial waste |
| title_full | Production and characterization of sophorolipid under yeast-mediated submerged fermentation utilizing Agro-industrial waste |
| title_fullStr | Production and characterization of sophorolipid under yeast-mediated submerged fermentation utilizing Agro-industrial waste |
| title_full_unstemmed | Production and characterization of sophorolipid under yeast-mediated submerged fermentation utilizing Agro-industrial waste |
| title_short | Production and characterization of sophorolipid under yeast-mediated submerged fermentation utilizing Agro-industrial waste |
| title_sort | production and characterization of sophorolipid under yeast mediated submerged fermentation utilizing agro industrial waste |
| topic | Agro-industrial waste Bagasse Sophorolipid Renewable resource |
| url | http://www.sciencedirect.com/science/article/pii/S2666517424001172 |
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