Construction and Encapsulation of Colloidal Vesicles Based on Bovine Serum Albumin-Dextran Interfacial Assembly
As colloidal structural components, the colloidal vesicles formed by the modified proteins and polysaccharides were a unique type of artificial cells with good membrane permeability, so they were widely used in cell engineering, biosensing, drug delivery and functional foods. Based on this, this stu...
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The editorial department of Science and Technology of Food Industry
2025-01-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024030066 |
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| author | Qianqian GUO Dan LI Yongli YANG Li LI Gaoyang YI Xiaolin YAO |
| author_facet | Qianqian GUO Dan LI Yongli YANG Li LI Gaoyang YI Xiaolin YAO |
| author_sort | Qianqian GUO |
| collection | DOAJ |
| description | As colloidal structural components, the colloidal vesicles formed by the modified proteins and polysaccharides were a unique type of artificial cells with good membrane permeability, so they were widely used in cell engineering, biosensing, drug delivery and functional foods. Based on this, this study aimed to construct colloidal vesicles with aminoized bovine serum albumin (BSA-NH2) and carboxylated dextran (DEX-COOH) as the building blocks, and use Pickering emulsion template method to optimize complex concentration, oil/water volume ratio and crosslinker concentration. The storage stability at different temperatures and the ability to contain dextran with different molecular weight were investigated. Results showed that colloidal vesicles with stable morphology, uniform dispersion, stable smooth spherical shape and complete membrane structure were obtained under the conditions of compound concentration of 20 mg/mL, oil/water volume ratio of 10:1 and crosslinker concentration of 0.75 mg/mL, with size of 4.29±1.11 μm. The vesicles were stable at 4 ℃ on 0, 4 and 7 d, with sizes of 5.13±1.15, 4.81±1.03 and 5.01±1.33 μm, respectively, and could effectively contain dextran with molecular weight ≤70 kDa. Therefore, the vesicles can be used as an effective carrier of hydrophilic active components and widely used in food active component delivery. |
| format | Article |
| id | doaj-art-5aeacc63de47448fb36a6a1443d89154 |
| institution | Kabale University |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2025-01-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj-art-5aeacc63de47448fb36a6a1443d891542025-01-10T06:49:30ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-01-014629410310.13386/j.issn1002-0306.20240300662024030066-2Construction and Encapsulation of Colloidal Vesicles Based on Bovine Serum Albumin-Dextran Interfacial AssemblyQianqian GUO0Dan LI1Yongli YANG2Li LI3Gaoyang YI4Xiaolin YAO5School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, ChinaSchool of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, ChinaSchool of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, ChinaSchool of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, ChinaSchool of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, ChinaSchool of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, ChinaAs colloidal structural components, the colloidal vesicles formed by the modified proteins and polysaccharides were a unique type of artificial cells with good membrane permeability, so they were widely used in cell engineering, biosensing, drug delivery and functional foods. Based on this, this study aimed to construct colloidal vesicles with aminoized bovine serum albumin (BSA-NH2) and carboxylated dextran (DEX-COOH) as the building blocks, and use Pickering emulsion template method to optimize complex concentration, oil/water volume ratio and crosslinker concentration. The storage stability at different temperatures and the ability to contain dextran with different molecular weight were investigated. Results showed that colloidal vesicles with stable morphology, uniform dispersion, stable smooth spherical shape and complete membrane structure were obtained under the conditions of compound concentration of 20 mg/mL, oil/water volume ratio of 10:1 and crosslinker concentration of 0.75 mg/mL, with size of 4.29±1.11 μm. The vesicles were stable at 4 ℃ on 0, 4 and 7 d, with sizes of 5.13±1.15, 4.81±1.03 and 5.01±1.33 μm, respectively, and could effectively contain dextran with molecular weight ≤70 kDa. Therefore, the vesicles can be used as an effective carrier of hydrophilic active components and widely used in food active component delivery.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024030066bovine serum albumindextranvesiclesstabilityencapsulation |
| spellingShingle | Qianqian GUO Dan LI Yongli YANG Li LI Gaoyang YI Xiaolin YAO Construction and Encapsulation of Colloidal Vesicles Based on Bovine Serum Albumin-Dextran Interfacial Assembly Shipin gongye ke-ji bovine serum albumin dextran vesicles stability encapsulation |
| title | Construction and Encapsulation of Colloidal Vesicles Based on Bovine Serum Albumin-Dextran Interfacial Assembly |
| title_full | Construction and Encapsulation of Colloidal Vesicles Based on Bovine Serum Albumin-Dextran Interfacial Assembly |
| title_fullStr | Construction and Encapsulation of Colloidal Vesicles Based on Bovine Serum Albumin-Dextran Interfacial Assembly |
| title_full_unstemmed | Construction and Encapsulation of Colloidal Vesicles Based on Bovine Serum Albumin-Dextran Interfacial Assembly |
| title_short | Construction and Encapsulation of Colloidal Vesicles Based on Bovine Serum Albumin-Dextran Interfacial Assembly |
| title_sort | construction and encapsulation of colloidal vesicles based on bovine serum albumin dextran interfacial assembly |
| topic | bovine serum albumin dextran vesicles stability encapsulation |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024030066 |
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