Identification of STN7-associated leaf senescence networks in thylakoid membranes of Arabidopsis
Summary: Photosynthetic protein complexes in thylakoid membranes disassemble during leaf senescence, leading to a decline in photosynthetic efficiency. However, the mechanisms by which these protein complexes in chloroplast thylakoid membranes modulate leaf senescence are poorly understood. This stu...
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Elsevier
2025-06-01
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| author | Seongsin Lee Jihwan Park Seunghoon Back Roshan Sharma Poudyal Dong-Gi Mun Jingi Bae Nayoung Gwak Sang-Won Lee Daehee Hwang Yumi Kim |
| author_facet | Seongsin Lee Jihwan Park Seunghoon Back Roshan Sharma Poudyal Dong-Gi Mun Jingi Bae Nayoung Gwak Sang-Won Lee Daehee Hwang Yumi Kim |
| author_sort | Seongsin Lee |
| collection | DOAJ |
| description | Summary: Photosynthetic protein complexes in thylakoid membranes disassemble during leaf senescence, leading to a decline in photosynthetic efficiency. However, the mechanisms by which these protein complexes in chloroplast thylakoid membranes modulate leaf senescence are poorly understood. This study investigated the roles of Arabidopsis thaliana STN7 and STN8 kinases during leaf senescence using proteomic tools, characterizing STN7 and STN8 overexpressors and loss-of-function mutants. During leaf senescence, proteins involved in photosynthesis-associated processes decreased, while those related to lipid metabolism and jasmonic acid–mediated stress responses increased. The abundance and phosphorylation of the thylakoid membrane proteome were regulated by STN7, but not by STN8. Thirty-four STN7 target proteins overlapped with senescence-associated proteins in the wild type, most of which are related to the photosynthetic protein apparatus. Three out of nine mutants defective in STN7 downstream targets (lfnr2, tsp9, and riq2) exhibited accelerated leaf senescence based on chlorophyll a/b ratio measurements and photochemical analyses. Photosynthetic complex formation was normal in all STN7 target protein loss-of-function mutants, indicating the absence of feedback regulation upstream of STN7 during photosynthetic complex assembly. Overall, the data reveal that STN7 is a major kinase component in signaling cascades that link photosynthetic complex formation and leaf senescence. |
| format | Article |
| id | doaj-art-5ae9c308e53444988d8fb618cfc8e280 |
| institution | OA Journals |
| issn | 2589-0042 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-5ae9c308e53444988d8fb618cfc8e2802025-08-20T02:30:10ZengElsevieriScience2589-00422025-06-0128611257710.1016/j.isci.2025.112577Identification of STN7-associated leaf senescence networks in thylakoid membranes of ArabidopsisSeongsin Lee0Jihwan Park1Seunghoon Back2Roshan Sharma Poudyal3Dong-Gi Mun4Jingi Bae5Nayoung Gwak6Sang-Won Lee7Daehee Hwang8Yumi Kim9Center for Plant Aging Research, Institute for Basic Science, Daegu 42988, Republic of KoreaDepartment of New Biology, Daegu Gyeongbuk Institute for Science and Technology (DGIST), Daegu 42988, Republic of KoreaDepartment of Chemistry and Center for Proteogenome Research, Korea University, Seoul 02843, Republic of KoreaCenter for Plant Aging Research, Institute for Basic Science, Daegu 42988, Republic of Korea; Department of Agricultural Biotechnology, National Institute of Agricultural Science, Rural Development Administration, Jeonju, Republic of KoreaDepartment of Chemistry and Center for Proteogenome Research, Korea University, Seoul 02843, Republic of KoreaDepartment of Chemistry and Center for Proteogenome Research, Korea University, Seoul 02843, Republic of KoreaKorean Genomics Center, Ulsan National Institute of Science and Technology (UNIST), Ulsan 689-798, Republic of KoreaDepartment of Chemistry and Center for Proteogenome Research, Korea University, Seoul 02843, Republic of Korea; Corresponding authorCenter for Plant Aging Research, Institute for Basic Science, Daegu 42988, Republic of Korea; School of Biological Sciences, Seoul National University, Seoul, Republic of Korea; Corresponding authorCenter for Plant Aging Research, Institute for Basic Science, Daegu 42988, Republic of Korea; Center for Genomic Integrity, Institute for Basic Science, Ulsan 689-798, Republic of Korea; Corresponding authorSummary: Photosynthetic protein complexes in thylakoid membranes disassemble during leaf senescence, leading to a decline in photosynthetic efficiency. However, the mechanisms by which these protein complexes in chloroplast thylakoid membranes modulate leaf senescence are poorly understood. This study investigated the roles of Arabidopsis thaliana STN7 and STN8 kinases during leaf senescence using proteomic tools, characterizing STN7 and STN8 overexpressors and loss-of-function mutants. During leaf senescence, proteins involved in photosynthesis-associated processes decreased, while those related to lipid metabolism and jasmonic acid–mediated stress responses increased. The abundance and phosphorylation of the thylakoid membrane proteome were regulated by STN7, but not by STN8. Thirty-four STN7 target proteins overlapped with senescence-associated proteins in the wild type, most of which are related to the photosynthetic protein apparatus. Three out of nine mutants defective in STN7 downstream targets (lfnr2, tsp9, and riq2) exhibited accelerated leaf senescence based on chlorophyll a/b ratio measurements and photochemical analyses. Photosynthetic complex formation was normal in all STN7 target protein loss-of-function mutants, indicating the absence of feedback regulation upstream of STN7 during photosynthetic complex assembly. Overall, the data reveal that STN7 is a major kinase component in signaling cascades that link photosynthetic complex formation and leaf senescence.http://www.sciencedirect.com/science/article/pii/S2589004225008387Plant biochemistryPlant BiologyPlant physiologyPlant development |
| spellingShingle | Seongsin Lee Jihwan Park Seunghoon Back Roshan Sharma Poudyal Dong-Gi Mun Jingi Bae Nayoung Gwak Sang-Won Lee Daehee Hwang Yumi Kim Identification of STN7-associated leaf senescence networks in thylakoid membranes of Arabidopsis iScience Plant biochemistry Plant Biology Plant physiology Plant development |
| title | Identification of STN7-associated leaf senescence networks in thylakoid membranes of Arabidopsis |
| title_full | Identification of STN7-associated leaf senescence networks in thylakoid membranes of Arabidopsis |
| title_fullStr | Identification of STN7-associated leaf senescence networks in thylakoid membranes of Arabidopsis |
| title_full_unstemmed | Identification of STN7-associated leaf senescence networks in thylakoid membranes of Arabidopsis |
| title_short | Identification of STN7-associated leaf senescence networks in thylakoid membranes of Arabidopsis |
| title_sort | identification of stn7 associated leaf senescence networks in thylakoid membranes of arabidopsis |
| topic | Plant biochemistry Plant Biology Plant physiology Plant development |
| url | http://www.sciencedirect.com/science/article/pii/S2589004225008387 |
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