BMAL1 attenuates intervertebral disc degeneration by activating the SIRT1/PGC-1α pathway: evidence from vitro studies
Abstract To explore the potential effects and the corresponding mechanisms of brain and muscle arnt-like protein-1 (BMAL1) on the progression of intervertebral disc degeneration (IVDD) in vitro studies. The expression of BMAL1, SIRT1 and PINK1 were evaluated by the method of siRNA/pcDNA in the immor...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-03-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-94029-7 |
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| author | Peiming Sang Yanyan Ma Xie Zhang Binhui Chen Fan He Neng Shen Jiangang Zhao |
| author_facet | Peiming Sang Yanyan Ma Xie Zhang Binhui Chen Fan He Neng Shen Jiangang Zhao |
| author_sort | Peiming Sang |
| collection | DOAJ |
| description | Abstract To explore the potential effects and the corresponding mechanisms of brain and muscle arnt-like protein-1 (BMAL1) on the progression of intervertebral disc degeneration (IVDD) in vitro studies. The expression of BMAL1, SIRT1 and PINK1 were evaluated by the method of siRNA/pcDNA in the immortalized nucleus pulposus (NP) cells. The expression of SIRT1/PGC-1α pathway was assessed. The characteristics of NP cell, containing the activity and density, the level of apoptosis, inflammatory response, reactive oxygen species (ROS), senescence, and mitophagy were evaluated. The overexpression of BMAL1 was achieved with the pcDNA3.1, the expression of SIRT1 and PGC-1α were increased, the inflammatory response, the ROS, the level of apoptosis and senescence were decreased, however, the level of mitophagy, the activity and density of NP cell were enhanced. The BMAL1 inhibites the progression of IVDD by activating the SIRT1/PGC-1α pathway in the vitro studies. |
| format | Article |
| id | doaj-art-5ae5e8aee2ef46d893a702b103c32d25 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-5ae5e8aee2ef46d893a702b103c32d252025-08-20T02:52:17ZengNature PortfolioScientific Reports2045-23222025-03-0115111310.1038/s41598-025-94029-7BMAL1 attenuates intervertebral disc degeneration by activating the SIRT1/PGC-1α pathway: evidence from vitro studiesPeiming Sang0Yanyan Ma1Xie Zhang2Binhui Chen3Fan He4Neng Shen5Jiangang Zhao6Ningbo Medical Center LiHuiLi HospitalNingbo Medical Center LiHuiLi HospitalNingbo Medical Center LiHuiLi HospitalNingbo Medical Center LiHuiLi HospitalNingbo Medical Center LiHuiLi HospitalThe NO. 3 Hospital of Yinzhou DistrictThe NO. 4 Hospital of Yuyao DistrictAbstract To explore the potential effects and the corresponding mechanisms of brain and muscle arnt-like protein-1 (BMAL1) on the progression of intervertebral disc degeneration (IVDD) in vitro studies. The expression of BMAL1, SIRT1 and PINK1 were evaluated by the method of siRNA/pcDNA in the immortalized nucleus pulposus (NP) cells. The expression of SIRT1/PGC-1α pathway was assessed. The characteristics of NP cell, containing the activity and density, the level of apoptosis, inflammatory response, reactive oxygen species (ROS), senescence, and mitophagy were evaluated. The overexpression of BMAL1 was achieved with the pcDNA3.1, the expression of SIRT1 and PGC-1α were increased, the inflammatory response, the ROS, the level of apoptosis and senescence were decreased, however, the level of mitophagy, the activity and density of NP cell were enhanced. The BMAL1 inhibites the progression of IVDD by activating the SIRT1/PGC-1α pathway in the vitro studies.https://doi.org/10.1038/s41598-025-94029-7BMAL1MitophagyIntervertebral disc degeneration (IVDD)SIRT1/PGC-1α |
| spellingShingle | Peiming Sang Yanyan Ma Xie Zhang Binhui Chen Fan He Neng Shen Jiangang Zhao BMAL1 attenuates intervertebral disc degeneration by activating the SIRT1/PGC-1α pathway: evidence from vitro studies Scientific Reports BMAL1 Mitophagy Intervertebral disc degeneration (IVDD) SIRT1/PGC-1α |
| title | BMAL1 attenuates intervertebral disc degeneration by activating the SIRT1/PGC-1α pathway: evidence from vitro studies |
| title_full | BMAL1 attenuates intervertebral disc degeneration by activating the SIRT1/PGC-1α pathway: evidence from vitro studies |
| title_fullStr | BMAL1 attenuates intervertebral disc degeneration by activating the SIRT1/PGC-1α pathway: evidence from vitro studies |
| title_full_unstemmed | BMAL1 attenuates intervertebral disc degeneration by activating the SIRT1/PGC-1α pathway: evidence from vitro studies |
| title_short | BMAL1 attenuates intervertebral disc degeneration by activating the SIRT1/PGC-1α pathway: evidence from vitro studies |
| title_sort | bmal1 attenuates intervertebral disc degeneration by activating the sirt1 pgc 1α pathway evidence from vitro studies |
| topic | BMAL1 Mitophagy Intervertebral disc degeneration (IVDD) SIRT1/PGC-1α |
| url | https://doi.org/10.1038/s41598-025-94029-7 |
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