Fluorescence imaging in evaluating the revascularization of heterotopically transplanted primate trachea segment
Objective: to assess the potentials of using indocyanine green fluorescence angiography in evaluating revascularization of tissue-engineered construct that was obtained from the decellularized biological matrix of primate trachea, including using mesenchymal stem cells, after heterotopic tracheal al...
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| Language: | Russian |
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Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov
2020-07-01
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| Series: | Вестник трансплантологии и искусственных органов |
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| Online Access: | https://journal.transpl.ru/vtio/article/view/1187 |
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| author | A. L. Akopov G. V. Papayan S. D. Gorbunkov S. V. Orlov D. D. Karal-Ogly P. A. Kaplanyan E. A. Gubareva E. V. Kuevda D. M. Kuznetsova |
| author_facet | A. L. Akopov G. V. Papayan S. D. Gorbunkov S. V. Orlov D. D. Karal-Ogly P. A. Kaplanyan E. A. Gubareva E. V. Kuevda D. M. Kuznetsova |
| author_sort | A. L. Akopov |
| collection | DOAJ |
| description | Objective: to assess the potentials of using indocyanine green fluorescence angiography in evaluating revascularization of tissue-engineered construct that was obtained from the decellularized biological matrix of primate trachea, including using mesenchymal stem cells, after heterotopic tracheal allotransplantation. Material and methods. Tracheas were obtained from two male hamadryas baboons. After decellularization, 4 cm segments of tracheas were implanted under the lateral part of the latissimus dorsi in two healthy primates, one after recellularization with mesenchymal stem cells (animal 1), and the second without recellularization (animal 2). Immunosuppressive therapy was not performed. Blood flow in the transplanted segment of the trachea was evaluated 60 days after transplantation by surgical isolation of the flap of the latissimus dorsi with the transplanted segment of the trachea, while maintaining blood flow through the thoracodorsal artery. Indocyanine green near-infrared fluorescence angiography was visualized using a FLUM-808 multispectral fluorescence organoscope. Results. Sixty days after implantation, the tracheal cartilaginous framework macroscopically appeared to be intact in both animals, tightly integrated into the muscle tissue. The framework retained its natural color. After intravenous injection of indocyanine green, the tracheal vessels were visualized in both animals. Intercartilaginous vessels and portions of the cartilaginous semi-rings devoid of vessels were clearly distinguished. The entire implanted segment was almost uniformly vascularized. No local disruptions in blood supply were observed. The fluorescence brightness of the tracheal vessels was 193 ± 17 cu and 198 ± 10 cu in animals 1 and 2, respectively. The average muscle brightness in the implantation zone was 159 ± 9 cu and 116 ± 8 cu in animals 1 and 2, respectively. Conclusion. Indocyanine green fluorescence angiography is characterized by high-contrast images and high sensitivity. This facilitates vascular patency visualization and allows to assess the degree of neoangiogenesis after experimental transplantation of the tracheal segment, at different stages of experiment, without euthanizing the animal. |
| format | Article |
| id | doaj-art-5ae48e9060894097b0f4c80a0ccd1c2c |
| institution | DOAJ |
| issn | 1995-1191 |
| language | Russian |
| publishDate | 2020-07-01 |
| publisher | Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov |
| record_format | Article |
| series | Вестник трансплантологии и искусственных органов |
| spelling | doaj-art-5ae48e9060894097b0f4c80a0ccd1c2c2025-08-20T03:21:06ZrusFederal Research Center of Transplantology and Artificial Organs named after V.I.ShumakovВестник трансплантологии и искусственных органов1995-11912020-07-01222808510.15825/1995-1191-2020-2-80-85875Fluorescence imaging in evaluating the revascularization of heterotopically transplanted primate trachea segmentA. L. Akopov0G. V. Papayan1S. D. Gorbunkov2S. V. Orlov3D. D. Karal-Ogly4P. A. Kaplanyan5E. A. Gubareva6E. V. Kuevda7D. M. Kuznetsova8Pavlov First St. Petersburg State Medical UniversityPavlov First St. Petersburg State Medical UniversityPavlov First St. Petersburg State Medical UniversityResearch Institute of Medical PrimatologyResearch Institute of Medical PrimatologyResearch Institute of Medical PrimatologyKuban State Medical UniversityKuban State Medical UniversityKuban State Medical UniversityObjective: to assess the potentials of using indocyanine green fluorescence angiography in evaluating revascularization of tissue-engineered construct that was obtained from the decellularized biological matrix of primate trachea, including using mesenchymal stem cells, after heterotopic tracheal allotransplantation. Material and methods. Tracheas were obtained from two male hamadryas baboons. After decellularization, 4 cm segments of tracheas were implanted under the lateral part of the latissimus dorsi in two healthy primates, one after recellularization with mesenchymal stem cells (animal 1), and the second without recellularization (animal 2). Immunosuppressive therapy was not performed. Blood flow in the transplanted segment of the trachea was evaluated 60 days after transplantation by surgical isolation of the flap of the latissimus dorsi with the transplanted segment of the trachea, while maintaining blood flow through the thoracodorsal artery. Indocyanine green near-infrared fluorescence angiography was visualized using a FLUM-808 multispectral fluorescence organoscope. Results. Sixty days after implantation, the tracheal cartilaginous framework macroscopically appeared to be intact in both animals, tightly integrated into the muscle tissue. The framework retained its natural color. After intravenous injection of indocyanine green, the tracheal vessels were visualized in both animals. Intercartilaginous vessels and portions of the cartilaginous semi-rings devoid of vessels were clearly distinguished. The entire implanted segment was almost uniformly vascularized. No local disruptions in blood supply were observed. The fluorescence brightness of the tracheal vessels was 193 ± 17 cu and 198 ± 10 cu in animals 1 and 2, respectively. The average muscle brightness in the implantation zone was 159 ± 9 cu and 116 ± 8 cu in animals 1 and 2, respectively. Conclusion. Indocyanine green fluorescence angiography is characterized by high-contrast images and high sensitivity. This facilitates vascular patency visualization and allows to assess the degree of neoangiogenesis after experimental transplantation of the tracheal segment, at different stages of experiment, without euthanizing the animal.https://journal.transpl.ru/vtio/article/view/1187tracheatissue engineeringtransplantationangiographyfluorescence |
| spellingShingle | A. L. Akopov G. V. Papayan S. D. Gorbunkov S. V. Orlov D. D. Karal-Ogly P. A. Kaplanyan E. A. Gubareva E. V. Kuevda D. M. Kuznetsova Fluorescence imaging in evaluating the revascularization of heterotopically transplanted primate trachea segment Вестник трансплантологии и искусственных органов trachea tissue engineering transplantation angiography fluorescence |
| title | Fluorescence imaging in evaluating the revascularization of heterotopically transplanted primate trachea segment |
| title_full | Fluorescence imaging in evaluating the revascularization of heterotopically transplanted primate trachea segment |
| title_fullStr | Fluorescence imaging in evaluating the revascularization of heterotopically transplanted primate trachea segment |
| title_full_unstemmed | Fluorescence imaging in evaluating the revascularization of heterotopically transplanted primate trachea segment |
| title_short | Fluorescence imaging in evaluating the revascularization of heterotopically transplanted primate trachea segment |
| title_sort | fluorescence imaging in evaluating the revascularization of heterotopically transplanted primate trachea segment |
| topic | trachea tissue engineering transplantation angiography fluorescence |
| url | https://journal.transpl.ru/vtio/article/view/1187 |
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