Molecular investigation of how drought stress affects chlorophyll metabolism and photosynthesis in leaves of C3 and C4 plant species: A transcriptome meta-analysis
Drought stress has a significant impact on photosynthesis in plants, leading to reduced photosynthesis rates and affecting plant growth and yield. Understanding the effects of drought stress on photosynthetic pathways, particularly in C3 and C4 plants, is crucial for maximizing agricultural producti...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-02-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844025007480 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832557514938384384 |
|---|---|
| author | Shima Karami Behrouz Shiran Rudabeh Ravash |
| author_facet | Shima Karami Behrouz Shiran Rudabeh Ravash |
| author_sort | Shima Karami |
| collection | DOAJ |
| description | Drought stress has a significant impact on photosynthesis in plants, leading to reduced photosynthesis rates and affecting plant growth and yield. Understanding the effects of drought stress on photosynthetic pathways, particularly in C3 and C4 plants, is crucial for maximizing agricultural productivity and maintaining food security. In this study, we analyzed RNA-seq data from leaves of common wheat (Triticum aestivum) and sorghum (Sorghum bicolor), as representatives of C3 and C4, using a meta-analysis approach to investigate the photosynthesis-related genes involved in the response to drought stress. We identified specific genes and components of the photosynthesis pathway that are affected by drought stress. The findings suggest that wheat and sorghum respond differently to drought stress, with sorghum showing a more effective defense system against photoinhibition and damage to photosystems. On the other hand, it seems that in wheat, in order to deal with oxidative stress, the expression of homologous genes of C4 enzyme and genes involved in heme and siroheme synthesis pathway has increased under stress. This is probably due to the higher photoinhibition in C3 photosynthetic system compared to C4. Furthermore, drought stress affected chlorophyll biosynthesis and degradation pathways in both wheat and sorghum, but compared with sorghum, drought stress had a greater inhibitory effect on chlorophyll biosynthesis in wheat, which indicates the difference in their ability to cope with photoinhibition. |
| format | Article |
| id | doaj-art-5ad33f52405d4681a873366aa472ab97 |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-5ad33f52405d4681a873366aa472ab972025-02-03T04:16:48ZengElsevierHeliyon2405-84402025-02-01113e42368Molecular investigation of how drought stress affects chlorophyll metabolism and photosynthesis in leaves of C3 and C4 plant species: A transcriptome meta-analysisShima Karami0Behrouz Shiran1Rudabeh Ravash2Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Shahrekord University, Shahrekord, IranDepartment of Plant Breeding and Biotechnology, Faculty of Agriculture, Shahrekord University, Shahrekord, Iran; Institute of Biotechnology, Shahrekord University, P.O. Box 115, Shahrekord, Iran; Corresponding author. Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Shahrekord University, P.O. Box 115, Shahrekord, Iran.Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Shahrekord University, Shahrekord, Iran; Corresponding author. Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Shahrekord University, P.O. Box 115, Shahrekord, Iran.Drought stress has a significant impact on photosynthesis in plants, leading to reduced photosynthesis rates and affecting plant growth and yield. Understanding the effects of drought stress on photosynthetic pathways, particularly in C3 and C4 plants, is crucial for maximizing agricultural productivity and maintaining food security. In this study, we analyzed RNA-seq data from leaves of common wheat (Triticum aestivum) and sorghum (Sorghum bicolor), as representatives of C3 and C4, using a meta-analysis approach to investigate the photosynthesis-related genes involved in the response to drought stress. We identified specific genes and components of the photosynthesis pathway that are affected by drought stress. The findings suggest that wheat and sorghum respond differently to drought stress, with sorghum showing a more effective defense system against photoinhibition and damage to photosystems. On the other hand, it seems that in wheat, in order to deal with oxidative stress, the expression of homologous genes of C4 enzyme and genes involved in heme and siroheme synthesis pathway has increased under stress. This is probably due to the higher photoinhibition in C3 photosynthetic system compared to C4. Furthermore, drought stress affected chlorophyll biosynthesis and degradation pathways in both wheat and sorghum, but compared with sorghum, drought stress had a greater inhibitory effect on chlorophyll biosynthesis in wheat, which indicates the difference in their ability to cope with photoinhibition.http://www.sciencedirect.com/science/article/pii/S2405844025007480PhotosynthesisC3C4DroughtMeta-analysis |
| spellingShingle | Shima Karami Behrouz Shiran Rudabeh Ravash Molecular investigation of how drought stress affects chlorophyll metabolism and photosynthesis in leaves of C3 and C4 plant species: A transcriptome meta-analysis Heliyon Photosynthesis C3 C4 Drought Meta-analysis |
| title | Molecular investigation of how drought stress affects chlorophyll metabolism and photosynthesis in leaves of C3 and C4 plant species: A transcriptome meta-analysis |
| title_full | Molecular investigation of how drought stress affects chlorophyll metabolism and photosynthesis in leaves of C3 and C4 plant species: A transcriptome meta-analysis |
| title_fullStr | Molecular investigation of how drought stress affects chlorophyll metabolism and photosynthesis in leaves of C3 and C4 plant species: A transcriptome meta-analysis |
| title_full_unstemmed | Molecular investigation of how drought stress affects chlorophyll metabolism and photosynthesis in leaves of C3 and C4 plant species: A transcriptome meta-analysis |
| title_short | Molecular investigation of how drought stress affects chlorophyll metabolism and photosynthesis in leaves of C3 and C4 plant species: A transcriptome meta-analysis |
| title_sort | molecular investigation of how drought stress affects chlorophyll metabolism and photosynthesis in leaves of c3 and c4 plant species a transcriptome meta analysis |
| topic | Photosynthesis C3 C4 Drought Meta-analysis |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025007480 |
| work_keys_str_mv | AT shimakarami molecularinvestigationofhowdroughtstressaffectschlorophyllmetabolismandphotosynthesisinleavesofc3andc4plantspeciesatranscriptomemetaanalysis AT behrouzshiran molecularinvestigationofhowdroughtstressaffectschlorophyllmetabolismandphotosynthesisinleavesofc3andc4plantspeciesatranscriptomemetaanalysis AT rudabehravash molecularinvestigationofhowdroughtstressaffectschlorophyllmetabolismandphotosynthesisinleavesofc3andc4plantspeciesatranscriptomemetaanalysis |