Method Development and Validation for Determination of Voglibose in Tablet Formulation Using LC-MS/MS
Voglibose is a potent α glucosidase inhibitor, used for the treatment of diabetes mellitus. For quantitative determination of voglibose in pharmaceutical formulations of low doses, simple, sensitive, accurate and precise LC-MS/MS method using electrospray ionization in positive mode was developed an...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2011/531762 |
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| author | Mithlesh Rajput Meenakshi Dahiya Premlata Kumari Kamini Kalra Manjeet Aggarwal R. K. Khandal |
| author_facet | Mithlesh Rajput Meenakshi Dahiya Premlata Kumari Kamini Kalra Manjeet Aggarwal R. K. Khandal |
| author_sort | Mithlesh Rajput |
| collection | DOAJ |
| description | Voglibose is a potent α glucosidase inhibitor, used for the treatment of diabetes mellitus. For quantitative determination of voglibose in pharmaceutical formulations of low doses, simple, sensitive, accurate and precise LC-MS/MS method using electrospray ionization in positive mode was developed and validated. The method was found linear in the concentration range of 25.0-1200 ηg/mL with a correlation coefficient of 0.9998. The limit of detection (LOD) of the method was found to be 1.5 ηg/mL and limit of quantitation (LOQ) was achieved at 3.0 ηg/mL. The recoveries of voglibose from spiked samples at different concentration levels were found in the range of 98-102%. The proposed method was found suitable for quantitation of voglibose and for the determination of uniformity of content of the dosage units of the tablet formulations. |
| format | Article |
| id | doaj-art-5ac3673eeee44423a88c320c8bd5e983 |
| institution | OA Journals |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-5ac3673eeee44423a88c320c8bd5e9832025-08-20T02:05:28ZengWileyE-Journal of Chemistry0973-49452090-98102011-01-01841770178310.1155/2011/531762Method Development and Validation for Determination of Voglibose in Tablet Formulation Using LC-MS/MSMithlesh Rajput0Meenakshi Dahiya1Premlata Kumari2Kamini Kalra3Manjeet Aggarwal4R. K. Khandal5Shriram Institute for Industrial Research, 19, University Road, Delhi-110 007, IndiaShriram Institute for Industrial Research, 19, University Road, Delhi-110 007, IndiaShriram Institute for Industrial Research, 19, University Road, Delhi-110 007, IndiaShriram Institute for Industrial Research, 19, University Road, Delhi-110 007, IndiaShriram Institute for Industrial Research, 19, University Road, Delhi-110 007, IndiaShriram Institute for Industrial Research, 19, University Road, Delhi-110 007, IndiaVoglibose is a potent α glucosidase inhibitor, used for the treatment of diabetes mellitus. For quantitative determination of voglibose in pharmaceutical formulations of low doses, simple, sensitive, accurate and precise LC-MS/MS method using electrospray ionization in positive mode was developed and validated. The method was found linear in the concentration range of 25.0-1200 ηg/mL with a correlation coefficient of 0.9998. The limit of detection (LOD) of the method was found to be 1.5 ηg/mL and limit of quantitation (LOQ) was achieved at 3.0 ηg/mL. The recoveries of voglibose from spiked samples at different concentration levels were found in the range of 98-102%. The proposed method was found suitable for quantitation of voglibose and for the determination of uniformity of content of the dosage units of the tablet formulations.http://dx.doi.org/10.1155/2011/531762 |
| spellingShingle | Mithlesh Rajput Meenakshi Dahiya Premlata Kumari Kamini Kalra Manjeet Aggarwal R. K. Khandal Method Development and Validation for Determination of Voglibose in Tablet Formulation Using LC-MS/MS E-Journal of Chemistry |
| title | Method Development and Validation for Determination of Voglibose in Tablet Formulation Using LC-MS/MS |
| title_full | Method Development and Validation for Determination of Voglibose in Tablet Formulation Using LC-MS/MS |
| title_fullStr | Method Development and Validation for Determination of Voglibose in Tablet Formulation Using LC-MS/MS |
| title_full_unstemmed | Method Development and Validation for Determination of Voglibose in Tablet Formulation Using LC-MS/MS |
| title_short | Method Development and Validation for Determination of Voglibose in Tablet Formulation Using LC-MS/MS |
| title_sort | method development and validation for determination of voglibose in tablet formulation using lc ms ms |
| url | http://dx.doi.org/10.1155/2011/531762 |
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