Study on the Differences in Fecal Metabolites and Microbial Diversity of Jiangshan Black-Bone Chickens with Different Earlobe Colors
The differences in earlobe color of Jiangshan black-bone chickens have been reported to be caused by the combined effects of melanin and collagen. In this study, we conducted LC-MS untargeted metabolomics and 16S rDNA diversity sequencing on the cecal contents of two types of earlobes: peacock green...
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2024-10-01
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| author | Zhijun Wang Shiru Li Xiangying Ding Xue Du Ayong Zhao |
| author_facet | Zhijun Wang Shiru Li Xiangying Ding Xue Du Ayong Zhao |
| author_sort | Zhijun Wang |
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| description | The differences in earlobe color of Jiangshan black-bone chickens have been reported to be caused by the combined effects of melanin and collagen. In this study, we conducted LC-MS untargeted metabolomics and 16S rDNA diversity sequencing on the cecal contents of two types of earlobes: peacock green (Blue and Green group) and dark reddish-purple (Black group). The metabolomic sequencing identified a total of 747 differential metabolites (DMs), in which the metabolites were primarily enriched in tyrosine and tryptophan metabolism pathways between peacock green and dark reddish-purple earlobes. There were 15 different bacterial taxa among the three groups of earlobes at the genus level, and correlation analysis between metabolites and microbes revealed that the DMs between peacock green and dark reddish-purple earlobes were positively correlated with the different bacterial taxa. In short, there are differences in gut microbiota and metabolites between Jiangshan black-bone chickens with peacock green earlobes and those with dark reddish-purple earlobes. Our results suggest that the bacterial phyla Firmicutes and Bacteroidota may influence melanin synthesis by affecting tryptophan metabolism, induced by 5-Methoxyindoleacetate, and tyrosine metabolism, induced by maleylacetoacetic acid and maleic acid, leading to differences in earlobe color. |
| format | Article |
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| spelling | doaj-art-5ac29eee2b744f7fb9a4bab940c637c32025-08-20T02:14:15ZengMDPI AGAnimals2076-26152024-10-011421306010.3390/ani14213060Study on the Differences in Fecal Metabolites and Microbial Diversity of Jiangshan Black-Bone Chickens with Different Earlobe ColorsZhijun Wang0Shiru Li1Xiangying Ding2Xue Du3Ayong Zhao4Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection & Internet Technology, College of Animal Science and Technology & College of Veterinary Medicine of Zhejiang Agriculture and Forestry University, Hangzhou 311300, ChinaKey Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection & Internet Technology, College of Animal Science and Technology & College of Veterinary Medicine of Zhejiang Agriculture and Forestry University, Hangzhou 311300, ChinaJiangshan Agriculture and Rural Bureau, Quzhou 324100, ChinaKey Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection & Internet Technology, College of Animal Science and Technology & College of Veterinary Medicine of Zhejiang Agriculture and Forestry University, Hangzhou 311300, ChinaKey Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection & Internet Technology, College of Animal Science and Technology & College of Veterinary Medicine of Zhejiang Agriculture and Forestry University, Hangzhou 311300, ChinaThe differences in earlobe color of Jiangshan black-bone chickens have been reported to be caused by the combined effects of melanin and collagen. In this study, we conducted LC-MS untargeted metabolomics and 16S rDNA diversity sequencing on the cecal contents of two types of earlobes: peacock green (Blue and Green group) and dark reddish-purple (Black group). The metabolomic sequencing identified a total of 747 differential metabolites (DMs), in which the metabolites were primarily enriched in tyrosine and tryptophan metabolism pathways between peacock green and dark reddish-purple earlobes. There were 15 different bacterial taxa among the three groups of earlobes at the genus level, and correlation analysis between metabolites and microbes revealed that the DMs between peacock green and dark reddish-purple earlobes were positively correlated with the different bacterial taxa. In short, there are differences in gut microbiota and metabolites between Jiangshan black-bone chickens with peacock green earlobes and those with dark reddish-purple earlobes. Our results suggest that the bacterial phyla Firmicutes and Bacteroidota may influence melanin synthesis by affecting tryptophan metabolism, induced by 5-Methoxyindoleacetate, and tyrosine metabolism, induced by maleylacetoacetic acid and maleic acid, leading to differences in earlobe color.https://www.mdpi.com/2076-2615/14/21/3060Jiangshan black-bone chickenearlobe colormetabolomemicrobial diversitymelanin |
| spellingShingle | Zhijun Wang Shiru Li Xiangying Ding Xue Du Ayong Zhao Study on the Differences in Fecal Metabolites and Microbial Diversity of Jiangshan Black-Bone Chickens with Different Earlobe Colors Animals Jiangshan black-bone chicken earlobe color metabolome microbial diversity melanin |
| title | Study on the Differences in Fecal Metabolites and Microbial Diversity of Jiangshan Black-Bone Chickens with Different Earlobe Colors |
| title_full | Study on the Differences in Fecal Metabolites and Microbial Diversity of Jiangshan Black-Bone Chickens with Different Earlobe Colors |
| title_fullStr | Study on the Differences in Fecal Metabolites and Microbial Diversity of Jiangshan Black-Bone Chickens with Different Earlobe Colors |
| title_full_unstemmed | Study on the Differences in Fecal Metabolites and Microbial Diversity of Jiangshan Black-Bone Chickens with Different Earlobe Colors |
| title_short | Study on the Differences in Fecal Metabolites and Microbial Diversity of Jiangshan Black-Bone Chickens with Different Earlobe Colors |
| title_sort | study on the differences in fecal metabolites and microbial diversity of jiangshan black bone chickens with different earlobe colors |
| topic | Jiangshan black-bone chicken earlobe color metabolome microbial diversity melanin |
| url | https://www.mdpi.com/2076-2615/14/21/3060 |
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