Creation of a minimal tiling path of genomic clones for Drosophila: provision of a common resource
On the basis of shotgun subclone libraries used in the sequencing of the Drosophila melanogaster genome, a minimal tiling path of subclones across much of the genome was determined. About 320,000 shotgun clones for chromosomes X(12–20), 2R, 2L, 3R, and 4 were available from the Berkeley Drosophila G...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2004-08-01
|
| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/04372MT01 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850152125908123648 |
|---|---|
| author | Volker Hollich Eric Johnson Eileen E. Furlong Boris Beckmann Joseph Carlson Susan E. Celniker Jörg D. Hoheisel |
| author_facet | Volker Hollich Eric Johnson Eileen E. Furlong Boris Beckmann Joseph Carlson Susan E. Celniker Jörg D. Hoheisel |
| author_sort | Volker Hollich |
| collection | DOAJ |
| description | On the basis of shotgun subclone libraries used in the sequencing of the Drosophila melanogaster genome, a minimal tiling path of subclones across much of the genome was determined. About 320,000 shotgun clones for chromosomes X(12–20), 2R, 2L, 3R, and 4 were available from the Berkeley Drosophila Genome Project. The clone inserts have an average length of 3.4 kb and are amenable to standard PCR amplification. The resulting tiling path covers 86.2% of chromosome X(12–20), 86.2% of chromosomal arm 2R, 79.0% of 2L, 89.6% of 3R, and 80.5% of chromosome 4. In total, the 25,135 clones represent 76.7 Mb—equivalent to about 67% of the genome —and would be suitable for producing a microarray on a single slide. |
| format | Article |
| id | doaj-art-5aba77b6246c4d22be37759d76f24c2e |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2004-08-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-5aba77b6246c4d22be37759d76f24c2e2025-08-20T02:26:03ZengTaylor & Francis GroupBioTechniques0736-62051940-98182004-08-0137228228410.2144/04372MT01Creation of a minimal tiling path of genomic clones for Drosophila: provision of a common resourceVolker Hollich0Eric Johnson1Eileen E. Furlong2Boris Beckmann3Joseph Carlson4Susan E. Celniker5Jörg D. Hoheisel61Deutsches Krebsforschungszentrum, Heidelberg, Germany2University of Oregon, Eugene, OR, USA3EMBL, Heidelberg, Germany1Deutsches Krebsforschungszentrum, Heidelberg, Germany4Lawrence Berkeley National Laboratory, Berkeley, CA, USA4Lawrence Berkeley National Laboratory, Berkeley, CA, USA1Deutsches Krebsforschungszentrum, Heidelberg, GermanyOn the basis of shotgun subclone libraries used in the sequencing of the Drosophila melanogaster genome, a minimal tiling path of subclones across much of the genome was determined. About 320,000 shotgun clones for chromosomes X(12–20), 2R, 2L, 3R, and 4 were available from the Berkeley Drosophila Genome Project. The clone inserts have an average length of 3.4 kb and are amenable to standard PCR amplification. The resulting tiling path covers 86.2% of chromosome X(12–20), 86.2% of chromosomal arm 2R, 79.0% of 2L, 89.6% of 3R, and 80.5% of chromosome 4. In total, the 25,135 clones represent 76.7 Mb—equivalent to about 67% of the genome —and would be suitable for producing a microarray on a single slide.https://www.future-science.com/doi/10.2144/04372MT01 |
| spellingShingle | Volker Hollich Eric Johnson Eileen E. Furlong Boris Beckmann Joseph Carlson Susan E. Celniker Jörg D. Hoheisel Creation of a minimal tiling path of genomic clones for Drosophila: provision of a common resource BioTechniques |
| title | Creation of a minimal tiling path of genomic clones for Drosophila: provision of a common resource |
| title_full | Creation of a minimal tiling path of genomic clones for Drosophila: provision of a common resource |
| title_fullStr | Creation of a minimal tiling path of genomic clones for Drosophila: provision of a common resource |
| title_full_unstemmed | Creation of a minimal tiling path of genomic clones for Drosophila: provision of a common resource |
| title_short | Creation of a minimal tiling path of genomic clones for Drosophila: provision of a common resource |
| title_sort | creation of a minimal tiling path of genomic clones for drosophila provision of a common resource |
| url | https://www.future-science.com/doi/10.2144/04372MT01 |
| work_keys_str_mv | AT volkerhollich creationofaminimaltilingpathofgenomicclonesfordrosophilaprovisionofacommonresource AT ericjohnson creationofaminimaltilingpathofgenomicclonesfordrosophilaprovisionofacommonresource AT eileenefurlong creationofaminimaltilingpathofgenomicclonesfordrosophilaprovisionofacommonresource AT borisbeckmann creationofaminimaltilingpathofgenomicclonesfordrosophilaprovisionofacommonresource AT josephcarlson creationofaminimaltilingpathofgenomicclonesfordrosophilaprovisionofacommonresource AT susanecelniker creationofaminimaltilingpathofgenomicclonesfordrosophilaprovisionofacommonresource AT jorgdhoheisel creationofaminimaltilingpathofgenomicclonesfordrosophilaprovisionofacommonresource |