CD137 Regulates NFATc1 Expression in Mouse VSMCs through TRAF6/NF-κB p65 Signaling Pathway
Our previous study proved that CD137-CD137L interaction can regulate the expression of NFATc1. Here, we investigated whether CD137 signaling regulates the expression of NFATc1 in mice VSMCs through TRAF6/NF-κB p65 pathway. Data shows that the CD137 expression can be stimulated by TNF-α in a time-dep...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2015/639780 |
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| author | Jinchuan Yan Yunjie Yin Wei Zhong Cuiping Wang Zhongqun Wang |
| author_facet | Jinchuan Yan Yunjie Yin Wei Zhong Cuiping Wang Zhongqun Wang |
| author_sort | Jinchuan Yan |
| collection | DOAJ |
| description | Our previous study proved that CD137-CD137L interaction can regulate the expression of NFATc1. Here, we investigated whether CD137 signaling regulates the expression of NFATc1 in mice VSMCs through TRAF6/NF-κB p65 pathway. Data shows that the CD137 expression can be stimulated by TNF-α in a time-dependent manner in mouse VSMCs. Knockdown of TRAF6 by siTRAF6 significantly attenuated agonist-CD137mAb induced increase of NF-κB p65 and NFATc1 in VSMCs. Pretreatment with a NF-κB inhibitor PDTC for 30 min inhibited the expression of p-p65 in both cytoplasm and nucleus in VSMCs. Thus, the protein level of NFATc1 can be suppressed through inhibition of p-p65. Finally, we also show that the levels of IL-2 and IL-6 can be increased by agonist-CD137 stimulation and decreased when NFATc1 was suppressed. Our data suggest that activated CD137 signaling regulates the expression of NFATc1 and its downstream factors through TRAF6/NF-κB p65 pathways in VSMCs. These findings provide a novel target for treatment of atherosclerosis. |
| format | Article |
| id | doaj-art-5ab920a4665847859603a7c816617234 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-5ab920a4665847859603a7c8166172342025-02-03T01:03:37ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/639780639780CD137 Regulates NFATc1 Expression in Mouse VSMCs through TRAF6/NF-κB p65 Signaling PathwayJinchuan Yan0Yunjie Yin1Wei Zhong2Cuiping Wang3Zhongqun Wang4Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, ChinaDepartment of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, ChinaDepartment of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, ChinaDepartment of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, ChinaDepartment of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, ChinaOur previous study proved that CD137-CD137L interaction can regulate the expression of NFATc1. Here, we investigated whether CD137 signaling regulates the expression of NFATc1 in mice VSMCs through TRAF6/NF-κB p65 pathway. Data shows that the CD137 expression can be stimulated by TNF-α in a time-dependent manner in mouse VSMCs. Knockdown of TRAF6 by siTRAF6 significantly attenuated agonist-CD137mAb induced increase of NF-κB p65 and NFATc1 in VSMCs. Pretreatment with a NF-κB inhibitor PDTC for 30 min inhibited the expression of p-p65 in both cytoplasm and nucleus in VSMCs. Thus, the protein level of NFATc1 can be suppressed through inhibition of p-p65. Finally, we also show that the levels of IL-2 and IL-6 can be increased by agonist-CD137 stimulation and decreased when NFATc1 was suppressed. Our data suggest that activated CD137 signaling regulates the expression of NFATc1 and its downstream factors through TRAF6/NF-κB p65 pathways in VSMCs. These findings provide a novel target for treatment of atherosclerosis.http://dx.doi.org/10.1155/2015/639780 |
| spellingShingle | Jinchuan Yan Yunjie Yin Wei Zhong Cuiping Wang Zhongqun Wang CD137 Regulates NFATc1 Expression in Mouse VSMCs through TRAF6/NF-κB p65 Signaling Pathway Mediators of Inflammation |
| title | CD137 Regulates NFATc1 Expression in Mouse VSMCs through TRAF6/NF-κB p65 Signaling Pathway |
| title_full | CD137 Regulates NFATc1 Expression in Mouse VSMCs through TRAF6/NF-κB p65 Signaling Pathway |
| title_fullStr | CD137 Regulates NFATc1 Expression in Mouse VSMCs through TRAF6/NF-κB p65 Signaling Pathway |
| title_full_unstemmed | CD137 Regulates NFATc1 Expression in Mouse VSMCs through TRAF6/NF-κB p65 Signaling Pathway |
| title_short | CD137 Regulates NFATc1 Expression in Mouse VSMCs through TRAF6/NF-κB p65 Signaling Pathway |
| title_sort | cd137 regulates nfatc1 expression in mouse vsmcs through traf6 nf κb p65 signaling pathway |
| url | http://dx.doi.org/10.1155/2015/639780 |
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