Evaluation of Quality of Nitrite-Free Fermented Roe Deer (<i>Capreolus capreolus</i>) Sausage with Addition of Ascorbic Acid and Reduced NaCl

This study aimed to investigate the possibility of producing fermented roe deer sausages using acid whey without the addition of sodium nitrite. Additionally, ascorbic acid was added to improve the oxidative stability of the product, and sodium chloride (NaCl) was partially replaced by potassium chl...

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Main Authors: Karolina M. Wójciak, Paulina Kęska, Miroslava Kačániová, Natália Čmiková, Elżbieta Solska, Agata Ogórek
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Foods
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Online Access:https://www.mdpi.com/2304-8158/13/23/3823
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author Karolina M. Wójciak
Paulina Kęska
Miroslava Kačániová
Natália Čmiková
Elżbieta Solska
Agata Ogórek
author_facet Karolina M. Wójciak
Paulina Kęska
Miroslava Kačániová
Natália Čmiková
Elżbieta Solska
Agata Ogórek
author_sort Karolina M. Wójciak
collection DOAJ
description This study aimed to investigate the possibility of producing fermented roe deer sausages using acid whey without the addition of sodium nitrite. Additionally, ascorbic acid was added to improve the oxidative stability of the product, and sodium chloride (NaCl) was partially replaced by potassium chloride (KCl) (7:3). The sausages were analyzed after fermentation (on day 30) and during post-production aging (i.e., 60 and 90 days after production at 4 °C) for their pH, water activity (a<sub>w</sub>), redox potential (ORP), thiobarbituric acid value (TBARS), and color parameters (CIE L*, a*, and b*). The microbiological status of the products was also profiled. During aging, the a<sub>w</sub> and pH values were significantly lower (<i>p</i> < 0.05) in the variant with the addition of ascorbic acid. In all samples with the addition of acid whey, an increase in the TBARS value compared to the variant with sodium nitrite was observed, but among them, the variant with the substitution of NaCl by KCl was characterized by the lowest intensity of lipid oxidation. During post-production aging, the effect of acid whey on the loss of redness (a*) of the roe deer sausages was confirmed, with the lowest a* observed in samples with the addition of ascorbic acid. A total of 281 and 219 isolates with high scores were identified in the fermented deer sausages after fermentation (30 days) and storage (90 days), respectively. The most frequently isolated species from the fermented roe deer sausages were from the <i>Latilactobacillu</i> genus (<i>Latilactobacillus curvatus, Lati-lactobacillus sakei</i> subsp. <i>carnosus)</i> and <i>Leuconostoc</i> genus (<i>Leuconostoc mesenteroides</i>, <i>L. mesenteroides</i> subsp. <i>dextrani-cum</i>, and <i>Leuconostoc mesenteroides subsp. mesenteroides</i>).
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spelling doaj-art-5a9acaee82fd45cda069a921435d5e1f2025-08-20T02:50:37ZengMDPI AGFoods2304-81582024-11-011323382310.3390/foods13233823Evaluation of Quality of Nitrite-Free Fermented Roe Deer (<i>Capreolus capreolus</i>) Sausage with Addition of Ascorbic Acid and Reduced NaClKarolina M. Wójciak0Paulina Kęska1Miroslava Kačániová2Natália Čmiková3Elżbieta Solska4Agata Ogórek5Department of Animal Food Technology, Faculty of Food Science and Biotechnology, University of Life Sciences in Lublin, Skromna 8, 20-704 Lublin, PolandDepartment of Animal Food Technology, Faculty of Food Science and Biotechnology, University of Life Sciences in Lublin, Skromna 8, 20-704 Lublin, PolandInstitute of Horticulture, Faculty of Horticulture and Landscape Engineering, Slovak University of Agriculture, Tr. A. Hlinku 2, 94976 Nitra, SlovakiaInstitute of Horticulture, Faculty of Horticulture and Landscape Engineering, Slovak University of Agriculture, Tr. A. Hlinku 2, 94976 Nitra, SlovakiaDepartment of Animal Food Technology, Faculty of Food Science and Biotechnology, University of Life Sciences in Lublin, Skromna 8, 20-704 Lublin, PolandDepartment of Animal Food Technology, Faculty of Food Science and Biotechnology, University of Life Sciences in Lublin, Skromna 8, 20-704 Lublin, PolandThis study aimed to investigate the possibility of producing fermented roe deer sausages using acid whey without the addition of sodium nitrite. Additionally, ascorbic acid was added to improve the oxidative stability of the product, and sodium chloride (NaCl) was partially replaced by potassium chloride (KCl) (7:3). The sausages were analyzed after fermentation (on day 30) and during post-production aging (i.e., 60 and 90 days after production at 4 °C) for their pH, water activity (a<sub>w</sub>), redox potential (ORP), thiobarbituric acid value (TBARS), and color parameters (CIE L*, a*, and b*). The microbiological status of the products was also profiled. During aging, the a<sub>w</sub> and pH values were significantly lower (<i>p</i> < 0.05) in the variant with the addition of ascorbic acid. In all samples with the addition of acid whey, an increase in the TBARS value compared to the variant with sodium nitrite was observed, but among them, the variant with the substitution of NaCl by KCl was characterized by the lowest intensity of lipid oxidation. During post-production aging, the effect of acid whey on the loss of redness (a*) of the roe deer sausages was confirmed, with the lowest a* observed in samples with the addition of ascorbic acid. A total of 281 and 219 isolates with high scores were identified in the fermented deer sausages after fermentation (30 days) and storage (90 days), respectively. The most frequently isolated species from the fermented roe deer sausages were from the <i>Latilactobacillu</i> genus (<i>Latilactobacillus curvatus, Lati-lactobacillus sakei</i> subsp. <i>carnosus)</i> and <i>Leuconostoc</i> genus (<i>Leuconostoc mesenteroides</i>, <i>L. mesenteroides</i> subsp. <i>dextrani-cum</i>, and <i>Leuconostoc mesenteroides subsp. mesenteroides</i>).https://www.mdpi.com/2304-8158/13/23/3823venisonroe deerfermented sausageTBARSmeat colormicrobial analysis
spellingShingle Karolina M. Wójciak
Paulina Kęska
Miroslava Kačániová
Natália Čmiková
Elżbieta Solska
Agata Ogórek
Evaluation of Quality of Nitrite-Free Fermented Roe Deer (<i>Capreolus capreolus</i>) Sausage with Addition of Ascorbic Acid and Reduced NaCl
Foods
venison
roe deer
fermented sausage
TBARS
meat color
microbial analysis
title Evaluation of Quality of Nitrite-Free Fermented Roe Deer (<i>Capreolus capreolus</i>) Sausage with Addition of Ascorbic Acid and Reduced NaCl
title_full Evaluation of Quality of Nitrite-Free Fermented Roe Deer (<i>Capreolus capreolus</i>) Sausage with Addition of Ascorbic Acid and Reduced NaCl
title_fullStr Evaluation of Quality of Nitrite-Free Fermented Roe Deer (<i>Capreolus capreolus</i>) Sausage with Addition of Ascorbic Acid and Reduced NaCl
title_full_unstemmed Evaluation of Quality of Nitrite-Free Fermented Roe Deer (<i>Capreolus capreolus</i>) Sausage with Addition of Ascorbic Acid and Reduced NaCl
title_short Evaluation of Quality of Nitrite-Free Fermented Roe Deer (<i>Capreolus capreolus</i>) Sausage with Addition of Ascorbic Acid and Reduced NaCl
title_sort evaluation of quality of nitrite free fermented roe deer i capreolus capreolus i sausage with addition of ascorbic acid and reduced nacl
topic venison
roe deer
fermented sausage
TBARS
meat color
microbial analysis
url https://www.mdpi.com/2304-8158/13/23/3823
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