Effect of Reducing Sugar and Total Nitrogen to Ethanol Production from Molasses by Saccharomyces cerevisiae
Indonesia's oil production has declined, while demand for derivative products is increasing. Objective of this research are to understand effect of reducing sugar and total nitrogen variation to ethanol production and fermentation efficiency, cell viability, acidity, temperature, dissolved oxyg...
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| Language: | English |
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University of Brawijaya
2014-12-01
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| Series: | Journal of Experimental Life Science |
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| Online Access: | https://jels.ub.ac.id/index.php/jels/article/view/134 |
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| author | Ekwan Nofa Wiratno Tri Ardyati Agustin Krisna Wardani |
| author_facet | Ekwan Nofa Wiratno Tri Ardyati Agustin Krisna Wardani |
| author_sort | Ekwan Nofa Wiratno |
| collection | DOAJ |
| description | Indonesia's oil production has declined, while demand for derivative products is increasing. Objective of this research are to understand effect of reducing sugar and total nitrogen variation to ethanol production and fermentation efficiency, cell viability, acidity, temperature, dissolved oxygen with molasses by Saccharomyces cerevisiae (SAF Instant). Step of this research consist of determination of reducing sugar, ethanol fermentation, total nitrogen determination, ethanol determination and data analysis. Treatment of reducing sugar (GR) and total nitrogen (N) (g.L-1) that are GR 100 N 0, GR 100 N 6, GR 100 N 10, GR 125 N 0, GR 125 N 6 and GR 125 N 10. Fermentation was carried out for 72 hours with three replications. Observation parameters every 24 hours are ethanol and reducing sugar concentration, temperature, acidity and dissolved oxygen. Highest ethanol resulted from GR 125 N 6 (3.68 g.L-1) and GR 100 N 6 (3.53 g.L-1). Low reducing sugar consumption inhibited by by-product of yeast metabolism and molasses chemical compound, lead leaves high sugar concentration (> 80 g.L-1). GR 100 N 6 and GR 125 N 6 have highest fermentation efficiency (69 and 57 %). There was no increase in temperature and decrease in pH significantly (α>0.05). Dissolved oxygen decreased significantly (α>0.05) at the early of fermentation and decrease until the end of fermentation. Total nitrogen 6 g g.L-1 has the highest fermentation efficiency. |
| format | Article |
| id | doaj-art-5a96b72ae35e49c2bd1adcdbb1edf698 |
| institution | DOAJ |
| issn | 2087-2852 2338-1655 |
| language | English |
| publishDate | 2014-12-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Experimental Life Science |
| spelling | doaj-art-5a96b72ae35e49c2bd1adcdbb1edf6982025-08-20T03:13:04ZengUniversity of BrawijayaJournal of Experimental Life Science2087-28522338-16552014-12-01425055https://doi.org/10.21776/ub.jels.2014.004.02.05Effect of Reducing Sugar and Total Nitrogen to Ethanol Production from Molasses by Saccharomyces cerevisiaeEkwan Nofa Wiratno0Tri Ardyati1Agustin Krisna Wardani2Universitas Brawijaya, Malang, IndonesiaUniversitas Brawijaya, Malang, IndonesiaUniversitas Brawijaya, Malang, IndonesiaIndonesia's oil production has declined, while demand for derivative products is increasing. Objective of this research are to understand effect of reducing sugar and total nitrogen variation to ethanol production and fermentation efficiency, cell viability, acidity, temperature, dissolved oxygen with molasses by Saccharomyces cerevisiae (SAF Instant). Step of this research consist of determination of reducing sugar, ethanol fermentation, total nitrogen determination, ethanol determination and data analysis. Treatment of reducing sugar (GR) and total nitrogen (N) (g.L-1) that are GR 100 N 0, GR 100 N 6, GR 100 N 10, GR 125 N 0, GR 125 N 6 and GR 125 N 10. Fermentation was carried out for 72 hours with three replications. Observation parameters every 24 hours are ethanol and reducing sugar concentration, temperature, acidity and dissolved oxygen. Highest ethanol resulted from GR 125 N 6 (3.68 g.L-1) and GR 100 N 6 (3.53 g.L-1). Low reducing sugar consumption inhibited by by-product of yeast metabolism and molasses chemical compound, lead leaves high sugar concentration (> 80 g.L-1). GR 100 N 6 and GR 125 N 6 have highest fermentation efficiency (69 and 57 %). There was no increase in temperature and decrease in pH significantly (α>0.05). Dissolved oxygen decreased significantly (α>0.05) at the early of fermentation and decrease until the end of fermentation. Total nitrogen 6 g g.L-1 has the highest fermentation efficiency.https://jels.ub.ac.id/index.php/jels/article/view/134ethanolmolassesreducing sugarsaccharomyces cerevisiaetotal nitrogen |
| spellingShingle | Ekwan Nofa Wiratno Tri Ardyati Agustin Krisna Wardani Effect of Reducing Sugar and Total Nitrogen to Ethanol Production from Molasses by Saccharomyces cerevisiae Journal of Experimental Life Science ethanol molasses reducing sugar saccharomyces cerevisiae total nitrogen |
| title | Effect of Reducing Sugar and Total Nitrogen to Ethanol Production from Molasses by Saccharomyces cerevisiae |
| title_full | Effect of Reducing Sugar and Total Nitrogen to Ethanol Production from Molasses by Saccharomyces cerevisiae |
| title_fullStr | Effect of Reducing Sugar and Total Nitrogen to Ethanol Production from Molasses by Saccharomyces cerevisiae |
| title_full_unstemmed | Effect of Reducing Sugar and Total Nitrogen to Ethanol Production from Molasses by Saccharomyces cerevisiae |
| title_short | Effect of Reducing Sugar and Total Nitrogen to Ethanol Production from Molasses by Saccharomyces cerevisiae |
| title_sort | effect of reducing sugar and total nitrogen to ethanol production from molasses by saccharomyces cerevisiae |
| topic | ethanol molasses reducing sugar saccharomyces cerevisiae total nitrogen |
| url | https://jels.ub.ac.id/index.php/jels/article/view/134 |
| work_keys_str_mv | AT ekwannofawiratno effectofreducingsugarandtotalnitrogentoethanolproductionfrommolassesbysaccharomycescerevisiae AT triardyati effectofreducingsugarandtotalnitrogentoethanolproductionfrommolassesbysaccharomycescerevisiae AT agustinkrisnawardani effectofreducingsugarandtotalnitrogentoethanolproductionfrommolassesbysaccharomycescerevisiae |