Antibacterial Activity of Traditional Medicinal Plants: Combating Antibiotics Resistance in Animal Wound Infections
ABSTRACT The rise of antibiotic resistance poses a significant challenge to veterinary medicine, particularly in the treatment of wound infections in animals. This study aimed to evaluate the antibacterial properties of traditional medicinal plants against four bacterial strains isolated from septic...
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| Format: | Article |
| Language: | English |
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Wiley
2025-05-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.70361 |
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| author | Dan Jia Safia Arbab Hanif Ullah Khalid J. Alzahrani Fuad M. Alzahrani Khalaf F. Alsharif Jiyu Zhang Ka Li |
| author_facet | Dan Jia Safia Arbab Hanif Ullah Khalid J. Alzahrani Fuad M. Alzahrani Khalaf F. Alsharif Jiyu Zhang Ka Li |
| author_sort | Dan Jia |
| collection | DOAJ |
| description | ABSTRACT The rise of antibiotic resistance poses a significant challenge to veterinary medicine, particularly in the treatment of wound infections in animals. This study aimed to evaluate the antibacterial properties of traditional medicinal plants against four bacterial strains isolated from septic animal wound infections and to compare their effectiveness with broad‐spectrum antibiotics. A total of 150 bacterial isolates were collected using sterile cotton swabs, cultured on nutrient and mannitol salt agar for growth and identified through catalase and coagulase tests. The study assessed antibiotic resistance and tested the bacterial isolates’ susceptibility to medicinal plants using the disc diffusion method. Among the isolates, Staphylococcus aureus was the most common pathogen, accounting for 26.6% (40 isolates), followed by Escherichia coli at 26% (39 isolates). Streptococcus spp. and Pseudomonas spp. were found in 20% (30 isolates) and 16% (25 isolates), respectively. Antibiogram analysis revealed notable antibiotic resistance, with S. aureus showing the highest resistance to Ciprofloxacin (60.5%) and Rifampicin (57.5%). E. coli exhibited 61.5% resistance to Ciprofloxacin and 56.4% to streptomycin. Streptococcus spp. had the highest resistance to Ciprofloxacin (53.3%), whereas Pseudomonas spp. showed the greatest resistance to Chloramphenicol (52%). Ethanol extracts of the medicinal plants, prepared using various solvents, were utilized for testing. Ethanol extracts of Loranthus acaciae and Cymbopogon proximus at concentrations of 60–90 µL demonstrated the largest inhibition zones, ranging from 55.5 ± 3.85 to 57.5 ± 2.5 mm against E. coli and S. aureus. Inhibition zones for Streptococcus spp. and Pseudomonas spp. ranged from 50 ± 2 to 48.3 ± 1.7 mm. In comparison, the standard antibiotics exhibited inhibition zones of 58.95 ± 2.55–60 ± 2.5 mm against S. aureus and E. coli, with slightly lower zones (51.65 ± 1.6–49 ± 1 mm) observed for Streptococcus spp. and Pseudomonas spp. These findings underscore the significant antibacterial activity of medicinal plants against multidrug‐resistant pathogens in animal wound infections, highlighting their potential to accelerate healing, reduce infection severity and provide a cost‐effective alternative to combat antibiotic resistance. |
| format | Article |
| id | doaj-art-5a94f54971504f90bc7ecb0705929eab |
| institution | OA Journals |
| issn | 2053-1095 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Wiley |
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| series | Veterinary Medicine and Science |
| spelling | doaj-art-5a94f54971504f90bc7ecb0705929eab2025-08-20T02:30:12ZengWileyVeterinary Medicine and Science2053-10952025-05-01113n/an/a10.1002/vms3.70361Antibacterial Activity of Traditional Medicinal Plants: Combating Antibiotics Resistance in Animal Wound InfectionsDan Jia0Safia Arbab1Hanif Ullah2Khalid J. Alzahrani3Fuad M. Alzahrani4Khalaf F. Alsharif5Jiyu Zhang6Ka Li7Outpatient Department West China Hospital, Sichuan University/West China School of Nursing, Sichuan University Chengdu ChinaLanzhou Institute of Husbandry and Pharmaceutical Sciences Chinese Academy of Agricultural Sciences Lanzhou ChinaMedicine and Engineering Interdisciplinary Research Laboratory of Nursing and Materials/Nursing Key Laboratory of Sichuan Province West China Hospital, Sichuan University/West China School of Nursing Sichuan University Chengdu Sichuan ChinaDepartment of Clinical Laboratories Sciences College of Applied Medical Sciences Taif University Taif Saudi ArabiaDepartment of Clinical Laboratories Sciences College of Applied Medical Sciences Taif University Taif Saudi ArabiaDepartment of Clinical Laboratories Sciences College of Applied Medical Sciences Taif University Taif Saudi ArabiaLanzhou Institute of Husbandry and Pharmaceutical Sciences Chinese Academy of Agricultural Sciences Lanzhou ChinaMedicine and Engineering Interdisciplinary Research Laboratory of Nursing and Materials/Nursing Key Laboratory of Sichuan Province West China Hospital, Sichuan University/West China School of Nursing Sichuan University Chengdu Sichuan ChinaABSTRACT The rise of antibiotic resistance poses a significant challenge to veterinary medicine, particularly in the treatment of wound infections in animals. This study aimed to evaluate the antibacterial properties of traditional medicinal plants against four bacterial strains isolated from septic animal wound infections and to compare their effectiveness with broad‐spectrum antibiotics. A total of 150 bacterial isolates were collected using sterile cotton swabs, cultured on nutrient and mannitol salt agar for growth and identified through catalase and coagulase tests. The study assessed antibiotic resistance and tested the bacterial isolates’ susceptibility to medicinal plants using the disc diffusion method. Among the isolates, Staphylococcus aureus was the most common pathogen, accounting for 26.6% (40 isolates), followed by Escherichia coli at 26% (39 isolates). Streptococcus spp. and Pseudomonas spp. were found in 20% (30 isolates) and 16% (25 isolates), respectively. Antibiogram analysis revealed notable antibiotic resistance, with S. aureus showing the highest resistance to Ciprofloxacin (60.5%) and Rifampicin (57.5%). E. coli exhibited 61.5% resistance to Ciprofloxacin and 56.4% to streptomycin. Streptococcus spp. had the highest resistance to Ciprofloxacin (53.3%), whereas Pseudomonas spp. showed the greatest resistance to Chloramphenicol (52%). Ethanol extracts of the medicinal plants, prepared using various solvents, were utilized for testing. Ethanol extracts of Loranthus acaciae and Cymbopogon proximus at concentrations of 60–90 µL demonstrated the largest inhibition zones, ranging from 55.5 ± 3.85 to 57.5 ± 2.5 mm against E. coli and S. aureus. Inhibition zones for Streptococcus spp. and Pseudomonas spp. ranged from 50 ± 2 to 48.3 ± 1.7 mm. In comparison, the standard antibiotics exhibited inhibition zones of 58.95 ± 2.55–60 ± 2.5 mm against S. aureus and E. coli, with slightly lower zones (51.65 ± 1.6–49 ± 1 mm) observed for Streptococcus spp. and Pseudomonas spp. These findings underscore the significant antibacterial activity of medicinal plants against multidrug‐resistant pathogens in animal wound infections, highlighting their potential to accelerate healing, reduce infection severity and provide a cost‐effective alternative to combat antibiotic resistance.https://doi.org/10.1002/vms3.70361animal woundantibioticsantimicrobialherbal medicinal plants |
| spellingShingle | Dan Jia Safia Arbab Hanif Ullah Khalid J. Alzahrani Fuad M. Alzahrani Khalaf F. Alsharif Jiyu Zhang Ka Li Antibacterial Activity of Traditional Medicinal Plants: Combating Antibiotics Resistance in Animal Wound Infections Veterinary Medicine and Science animal wound antibiotics antimicrobial herbal medicinal plants |
| title | Antibacterial Activity of Traditional Medicinal Plants: Combating Antibiotics Resistance in Animal Wound Infections |
| title_full | Antibacterial Activity of Traditional Medicinal Plants: Combating Antibiotics Resistance in Animal Wound Infections |
| title_fullStr | Antibacterial Activity of Traditional Medicinal Plants: Combating Antibiotics Resistance in Animal Wound Infections |
| title_full_unstemmed | Antibacterial Activity of Traditional Medicinal Plants: Combating Antibiotics Resistance in Animal Wound Infections |
| title_short | Antibacterial Activity of Traditional Medicinal Plants: Combating Antibiotics Resistance in Animal Wound Infections |
| title_sort | antibacterial activity of traditional medicinal plants combating antibiotics resistance in animal wound infections |
| topic | animal wound antibiotics antimicrobial herbal medicinal plants |
| url | https://doi.org/10.1002/vms3.70361 |
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