Identifying bacterial and fungal communities associated with Fusarium-wilt symptomatic and non-symptomatic ‘Gros Michel’ banana plants in Ecuador
Fusarium wilt of banana (FWB), caused by Fusarium oxysporum f. sp. cubense (Foc), remains a critical threat to banana production worldwide. Despite the persistence of the disease in fields planted with susceptible cultivars such as ‘Gros Michel’, little is known about the microbial interactions infl...
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1572860/full |
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| author | Estefany M. Paredes Salgado Estefany M. Paredes Salgado Fiama E. Guevara Carlos Muentes Francisco J. Flores Francisco J. Flores Freddy Magdama Freddy Magdama |
| author_facet | Estefany M. Paredes Salgado Estefany M. Paredes Salgado Fiama E. Guevara Carlos Muentes Francisco J. Flores Francisco J. Flores Freddy Magdama Freddy Magdama |
| author_sort | Estefany M. Paredes Salgado |
| collection | DOAJ |
| description | Fusarium wilt of banana (FWB), caused by Fusarium oxysporum f. sp. cubense (Foc), remains a critical threat to banana production worldwide. Despite the persistence of the disease in fields planted with susceptible cultivars such as ‘Gros Michel’, little is known about the microbial interactions influencing symptom development. In this study, we assessed the bacterial and fungal communities associated to symptomatic and non-symptomatic ‘Gros Michel’ bananas plants sampled in Ecuador banana fields affected by Foc race 1. We aimed to compare their diversity, composition, and to identify potential microbial taxa that could be active in disease suppression. Samples were collected from the pseudostem, rhizome, and rhizosphere, and analyzed through high-throughput sequencing of the 16S rRNA and ITS2 regions to characterize bacterial and fungal communities, respectively. Results revealed that non-symptomatic plants harbored significantly higher bacterial diversity, particularly in pseudostem and rhizome tissues, compared to symptomatic plants. Genera including, Bacillus, Enterobacter, Paenibacillus, Pectobacterium, Herbaspirillum and Pseudomonas were enriched in non-symptomatic tissues, suggesting a potential role in disease suppression. In contrast, symptomatic plants showed an increased abundance of genera such as Klebsiella and Kosakonia. Fungal community shifts were less pronounced, indicating that bacterial dynamics may play a more critical role in disease development. These findings shed light on the key microbial taxa associated with FWB-affected banana plants and the potential role of their microbiome to plant health and disease suppression. |
| format | Article |
| id | doaj-art-5a904f165ee44f3ab51ebdf540d72165 |
| institution | Kabale University |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-5a904f165ee44f3ab51ebdf540d721652025-08-20T03:47:19ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-06-011510.3389/fcimb.2025.15728601572860Identifying bacterial and fungal communities associated with Fusarium-wilt symptomatic and non-symptomatic ‘Gros Michel’ banana plants in EcuadorEstefany M. Paredes Salgado0Estefany M. Paredes Salgado1Fiama E. Guevara2Carlos Muentes3Francisco J. Flores4Francisco J. Flores5Freddy Magdama6Freddy Magdama7Departamento de Ciencias de la Vida y la Agricultura, Universidad de las Fuerzas Armadas (UFA) Universidad de las Fuerzas Armadas (ESPE), Sangolquí, EcuadorEscuela Superior Politécnica del Litoral (ESPOL), Centro de Investigaciones Biotecnológicas del Ecuador (CIBE), Guayaquil, EcuadorCentro de Estudios de Posgrado de la Universidad de las Fuerzas Armadas (ESPE), Sangolquí, EcuadorAgencia de Regulación y Control Fito y Zoosanitario (AGROCALIDAD), Quito, EcuadorDepartamento de Ciencias de la Vida y la Agricultura, Universidad de las Fuerzas Armadas (UFA) Universidad de las Fuerzas Armadas (ESPE), Sangolquí, EcuadorCentro de Investigación de Alimentos, Facultad de Ciencias de la Ingeniería e Industrias, Universidad UTE, Quito, EcuadorEscuela Superior Politécnica del Litoral (ESPOL), Centro de Investigaciones Biotecnológicas del Ecuador (CIBE), Guayaquil, EcuadorFacultad de Ciencias de la Vida, Escuela Superior Politécnica del Litoral (ESPOL), Guayaquil, EcuadorFusarium wilt of banana (FWB), caused by Fusarium oxysporum f. sp. cubense (Foc), remains a critical threat to banana production worldwide. Despite the persistence of the disease in fields planted with susceptible cultivars such as ‘Gros Michel’, little is known about the microbial interactions influencing symptom development. In this study, we assessed the bacterial and fungal communities associated to symptomatic and non-symptomatic ‘Gros Michel’ bananas plants sampled in Ecuador banana fields affected by Foc race 1. We aimed to compare their diversity, composition, and to identify potential microbial taxa that could be active in disease suppression. Samples were collected from the pseudostem, rhizome, and rhizosphere, and analyzed through high-throughput sequencing of the 16S rRNA and ITS2 regions to characterize bacterial and fungal communities, respectively. Results revealed that non-symptomatic plants harbored significantly higher bacterial diversity, particularly in pseudostem and rhizome tissues, compared to symptomatic plants. Genera including, Bacillus, Enterobacter, Paenibacillus, Pectobacterium, Herbaspirillum and Pseudomonas were enriched in non-symptomatic tissues, suggesting a potential role in disease suppression. In contrast, symptomatic plants showed an increased abundance of genera such as Klebsiella and Kosakonia. Fungal community shifts were less pronounced, indicating that bacterial dynamics may play a more critical role in disease development. These findings shed light on the key microbial taxa associated with FWB-affected banana plants and the potential role of their microbiome to plant health and disease suppression.https://www.frontiersin.org/articles/10.3389/fcimb.2025.1572860/fullmicrobiome analysisMusa acuminata ‘Gros Michel’Fusarium oxysporum f. sp. cubenserhizome and rhizosphere soilendophytic microbessoil-borne fungal diseases |
| spellingShingle | Estefany M. Paredes Salgado Estefany M. Paredes Salgado Fiama E. Guevara Carlos Muentes Francisco J. Flores Francisco J. Flores Freddy Magdama Freddy Magdama Identifying bacterial and fungal communities associated with Fusarium-wilt symptomatic and non-symptomatic ‘Gros Michel’ banana plants in Ecuador Frontiers in Cellular and Infection Microbiology microbiome analysis Musa acuminata ‘Gros Michel’ Fusarium oxysporum f. sp. cubense rhizome and rhizosphere soil endophytic microbes soil-borne fungal diseases |
| title | Identifying bacterial and fungal communities associated with Fusarium-wilt symptomatic and non-symptomatic ‘Gros Michel’ banana plants in Ecuador |
| title_full | Identifying bacterial and fungal communities associated with Fusarium-wilt symptomatic and non-symptomatic ‘Gros Michel’ banana plants in Ecuador |
| title_fullStr | Identifying bacterial and fungal communities associated with Fusarium-wilt symptomatic and non-symptomatic ‘Gros Michel’ banana plants in Ecuador |
| title_full_unstemmed | Identifying bacterial and fungal communities associated with Fusarium-wilt symptomatic and non-symptomatic ‘Gros Michel’ banana plants in Ecuador |
| title_short | Identifying bacterial and fungal communities associated with Fusarium-wilt symptomatic and non-symptomatic ‘Gros Michel’ banana plants in Ecuador |
| title_sort | identifying bacterial and fungal communities associated with fusarium wilt symptomatic and non symptomatic gros michel banana plants in ecuador |
| topic | microbiome analysis Musa acuminata ‘Gros Michel’ Fusarium oxysporum f. sp. cubense rhizome and rhizosphere soil endophytic microbes soil-borne fungal diseases |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1572860/full |
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