Acalypha indica aqueous leaf extract as potential nematicide against the root-knot nematode, Meloidogyne incognita: in vitro and molecular docking studies
Root-knot nematode (RKN) (Meloidogyne incognita) is a major plant parasitic nematode that severely damages crops, leading to significant yield losses and substantial economic impact globally. This study aims to investigate an environmentally sustainable biological strategy for mitigating parasitic p...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
|
| Series: | Cogent Food & Agriculture |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/23311932.2024.2405027 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850247705408831488 |
|---|---|
| author | Altaf Khan Manar Fawzi Bani Mfarrej Fohad Mabood Husain Qamar Zia Mohd Adil Mohammed Arshad Khalid Umar Niraj Kumar Jha Pieter Malan Soumya Pandit Faheem Ahmad |
| author_facet | Altaf Khan Manar Fawzi Bani Mfarrej Fohad Mabood Husain Qamar Zia Mohd Adil Mohammed Arshad Khalid Umar Niraj Kumar Jha Pieter Malan Soumya Pandit Faheem Ahmad |
| author_sort | Altaf Khan |
| collection | DOAJ |
| description | Root-knot nematode (RKN) (Meloidogyne incognita) is a major plant parasitic nematode that severely damages crops, leading to significant yield losses and substantial economic impact globally. This study aims to investigate an environmentally sustainable biological strategy for mitigating parasitic populations of the root-knot nematode, M. incognita. Specifically, the research focuses on assessing the nematicidal efficacy of Acalypha indica against M. incognita mortality and second-stage juveniles’ (J2) hatching under controlled in vitro conditions. A. indica leaf aqueous extract was applied at varying concentrations (250, 500, 750, and 1000 ppm) to J2s and egg masses of M. incognita. Notably, at 1000 ppm, a significant increase in J2 mortality and hatching inhibition was observed, while 250 ppm concentration showed the least favorable outcome; with mortality rates ranging from 22–82%. Chemical analysis via gas chromatography-mass spectroscopy (GC-MS) identified Benzoic acid, Cyclooctasiloxane, and 3-Isopropoxy-1,1,1,7,7,7-hexamethyl-3,5,5-tris (trimethylsiloxy) tetrasiloxane as predominant compounds. The nematicidal activity of A. indica leaf extract was further validated through in silico molecular docking, revealing that benzoic acid, Cyclooctasiloxane, and 3-Isopropoxy-1,1,1,7,7,7-hexamethyl-3,5,5-tris (trimethylsiloxy) tetrasiloxane bind to the ODR 3 protein of M. incognita with binding energies of −15.72, −8.91, and −7.35 kJ/mol, respectively. These findings hold promise for environmentally benign root-knot nematode management, contributing to improved soil health. |
| format | Article |
| id | doaj-art-5a7edc0a56ad4346854cd8a0ce29afc6 |
| institution | OA Journals |
| issn | 2331-1932 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Cogent Food & Agriculture |
| spelling | doaj-art-5a7edc0a56ad4346854cd8a0ce29afc62025-08-20T01:58:52ZengTaylor & Francis GroupCogent Food & Agriculture2331-19322024-12-0110110.1080/23311932.2024.2405027Acalypha indica aqueous leaf extract as potential nematicide against the root-knot nematode, Meloidogyne incognita: in vitro and molecular docking studiesAltaf Khan0Manar Fawzi Bani Mfarrej1Fohad Mabood Husain2Qamar Zia3Mohd Adil4Mohammed Arshad5Khalid Umar6Niraj Kumar Jha7Pieter Malan8Soumya Pandit9Faheem Ahmad10Department of Pharmacology, College of Pharmacy, King Saud University, Riyadh, KSADepartment of Life and Environmental Sciences, College of Natural and Health Sciences, Zayed University, United Arab EmiratesDepartment of Food Science and Nutrition, King Saud University, Riyadh, KSAInterdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, IndiaDepartment of Environmental Sciences, Dalhousie University, Truro, Nova Scotia, CanadaDental Health Department, College of Applied Medical Sciences, King Saud University, Riyadh, KSASchool of Chemical Sciences, Universiti Sains Malaysia, Gelugor, Pulau Pinang, MalaysiaCentre for Global Health Research, Saveetha Medical College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IndiaUnit for Environmental Sciences and Management, Mafikeng Campus, North-West University, Mmabatho, South AfricaDepartment of Biotechnology, Graphic Era University, Dehradun, Uttarakhand, IndiaDepartment of Botany, Aligarh Muslim University, Aligarh, IndiaRoot-knot nematode (RKN) (Meloidogyne incognita) is a major plant parasitic nematode that severely damages crops, leading to significant yield losses and substantial economic impact globally. This study aims to investigate an environmentally sustainable biological strategy for mitigating parasitic populations of the root-knot nematode, M. incognita. Specifically, the research focuses on assessing the nematicidal efficacy of Acalypha indica against M. incognita mortality and second-stage juveniles’ (J2) hatching under controlled in vitro conditions. A. indica leaf aqueous extract was applied at varying concentrations (250, 500, 750, and 1000 ppm) to J2s and egg masses of M. incognita. Notably, at 1000 ppm, a significant increase in J2 mortality and hatching inhibition was observed, while 250 ppm concentration showed the least favorable outcome; with mortality rates ranging from 22–82%. Chemical analysis via gas chromatography-mass spectroscopy (GC-MS) identified Benzoic acid, Cyclooctasiloxane, and 3-Isopropoxy-1,1,1,7,7,7-hexamethyl-3,5,5-tris (trimethylsiloxy) tetrasiloxane as predominant compounds. The nematicidal activity of A. indica leaf extract was further validated through in silico molecular docking, revealing that benzoic acid, Cyclooctasiloxane, and 3-Isopropoxy-1,1,1,7,7,7-hexamethyl-3,5,5-tris (trimethylsiloxy) tetrasiloxane bind to the ODR 3 protein of M. incognita with binding energies of −15.72, −8.91, and −7.35 kJ/mol, respectively. These findings hold promise for environmentally benign root-knot nematode management, contributing to improved soil health.https://www.tandfonline.com/doi/10.1080/23311932.2024.2405027BotanicalsAcalypha indicamolecular dockingMeloidogyne incognitanematicidal activityPlant Pathology |
| spellingShingle | Altaf Khan Manar Fawzi Bani Mfarrej Fohad Mabood Husain Qamar Zia Mohd Adil Mohammed Arshad Khalid Umar Niraj Kumar Jha Pieter Malan Soumya Pandit Faheem Ahmad Acalypha indica aqueous leaf extract as potential nematicide against the root-knot nematode, Meloidogyne incognita: in vitro and molecular docking studies Cogent Food & Agriculture Botanicals Acalypha indica molecular docking Meloidogyne incognita nematicidal activity Plant Pathology |
| title | Acalypha indica aqueous leaf extract as potential nematicide against the root-knot nematode, Meloidogyne incognita: in vitro and molecular docking studies |
| title_full | Acalypha indica aqueous leaf extract as potential nematicide against the root-knot nematode, Meloidogyne incognita: in vitro and molecular docking studies |
| title_fullStr | Acalypha indica aqueous leaf extract as potential nematicide against the root-knot nematode, Meloidogyne incognita: in vitro and molecular docking studies |
| title_full_unstemmed | Acalypha indica aqueous leaf extract as potential nematicide against the root-knot nematode, Meloidogyne incognita: in vitro and molecular docking studies |
| title_short | Acalypha indica aqueous leaf extract as potential nematicide against the root-knot nematode, Meloidogyne incognita: in vitro and molecular docking studies |
| title_sort | acalypha indica aqueous leaf extract as potential nematicide against the root knot nematode meloidogyne incognita in vitro and molecular docking studies |
| topic | Botanicals Acalypha indica molecular docking Meloidogyne incognita nematicidal activity Plant Pathology |
| url | https://www.tandfonline.com/doi/10.1080/23311932.2024.2405027 |
| work_keys_str_mv | AT altafkhan acalyphaindicaaqueousleafextractaspotentialnematicideagainsttherootknotnematodemeloidogyneincognitainvitroandmoleculardockingstudies AT manarfawzibanimfarrej acalyphaindicaaqueousleafextractaspotentialnematicideagainsttherootknotnematodemeloidogyneincognitainvitroandmoleculardockingstudies AT fohadmaboodhusain acalyphaindicaaqueousleafextractaspotentialnematicideagainsttherootknotnematodemeloidogyneincognitainvitroandmoleculardockingstudies AT qamarzia acalyphaindicaaqueousleafextractaspotentialnematicideagainsttherootknotnematodemeloidogyneincognitainvitroandmoleculardockingstudies AT mohdadil acalyphaindicaaqueousleafextractaspotentialnematicideagainsttherootknotnematodemeloidogyneincognitainvitroandmoleculardockingstudies AT mohammedarshad acalyphaindicaaqueousleafextractaspotentialnematicideagainsttherootknotnematodemeloidogyneincognitainvitroandmoleculardockingstudies AT khalidumar acalyphaindicaaqueousleafextractaspotentialnematicideagainsttherootknotnematodemeloidogyneincognitainvitroandmoleculardockingstudies AT nirajkumarjha acalyphaindicaaqueousleafextractaspotentialnematicideagainsttherootknotnematodemeloidogyneincognitainvitroandmoleculardockingstudies AT pietermalan acalyphaindicaaqueousleafextractaspotentialnematicideagainsttherootknotnematodemeloidogyneincognitainvitroandmoleculardockingstudies AT soumyapandit acalyphaindicaaqueousleafextractaspotentialnematicideagainsttherootknotnematodemeloidogyneincognitainvitroandmoleculardockingstudies AT faheemahmad acalyphaindicaaqueousleafextractaspotentialnematicideagainsttherootknotnematodemeloidogyneincognitainvitroandmoleculardockingstudies |