Interfacial cfDNA Enrichment and Amplification with On‐Chip Thermoplasmonics for Highly Sensitive Cancerous Liquid Biopsy
Abstract Tumor‐derived cell‐free DNA (cfDNA) has been exploited as an effective liquid biopsy biomarker for early cancer diagnosis. However, the fragmented and low‐abundance nature in circulating blood pose challenges for highly sensitive cfDNA quantification. Herein, a multifunctional plasmonic bio...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-01-01
|
| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202409708 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832582748207841280 |
|---|---|
| author | Danhua Wang Linlin Liu Wenjing Chi Zhenping Liu Jiayun Wu Yirou Liang Fei He Ruixiang Zhang Pengxin Huang Yunbo Li Guangyu Qiu |
| author_facet | Danhua Wang Linlin Liu Wenjing Chi Zhenping Liu Jiayun Wu Yirou Liang Fei He Ruixiang Zhang Pengxin Huang Yunbo Li Guangyu Qiu |
| author_sort | Danhua Wang |
| collection | DOAJ |
| description | Abstract Tumor‐derived cell‐free DNA (cfDNA) has been exploited as an effective liquid biopsy biomarker for early cancer diagnosis. However, the fragmented and low‐abundance nature in circulating blood pose challenges for highly sensitive cfDNA quantification. Herein, a multifunctional plasmonic biosensor termed Interfacial cfDNA Enrichment, Amplification and Sensing with on‐chip Thermoplasmonics (INEAST) is developed for cfDNA‐based liquid biopsy and lung cancer diagnosis. The INEAST biosensor achieved in situ thermoregulation and label‐free cfDNA biosensing by simultaneously harnessing interfacial thermoplasmonics and localized surface plasmon resonance. Typical cfDNA biomarkers, including epidermal growth factor receptor (EGFR), tumor protein 53 (TP53), phosphatase and tensin homologue deleted on chromosome 10 (PTEN), and cyclin‐dependent kinase inhibitor (CDKN2A), are quantified with detection limits down to femtomolar‐level. Through further validation using blood samples from lung cancer patients, the proposed INEAST bioassays demonstrated superior reliability for lung cancer screening, particularly when combined with clinically available tumor‐protein metrics. This study demonstrated that the INEAST biosensor enables rapid and sensitive cfDNA quantification, yielding a promising and compatible liquid biopsy for early‐stage lung cancer diagnosis. |
| format | Article |
| id | doaj-art-5a7924aec41e4dca8efabad770fd8adc |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-5a7924aec41e4dca8efabad770fd8adc2025-01-29T09:50:19ZengWileyAdvanced Science2198-38442025-01-01124n/an/a10.1002/advs.202409708Interfacial cfDNA Enrichment and Amplification with On‐Chip Thermoplasmonics for Highly Sensitive Cancerous Liquid BiopsyDanhua Wang0Linlin Liu1Wenjing Chi2Zhenping Liu3Jiayun Wu4Yirou Liang5Fei He6Ruixiang Zhang7Pengxin Huang8Yunbo Li9Guangyu Qiu10Institute of Medical Robotics, School of Biomedical Engineering Shanghai Jiao Tong University Shanghai 200240 ChinaInstitute of Medical Robotics, School of Biomedical Engineering Shanghai Jiao Tong University Shanghai 200240 ChinaDepartment of Laboratory Medicine Huadong Hospital Affiliated to Fudan University Shanghai 200031 ChinaThe First People's Hospital of Linping District Hangzhou Zhejiang Province 311100 ChinaInstitute of Medical Robotics, School of Biomedical Engineering Shanghai Jiao Tong University Shanghai 200240 ChinaInstitute of Medical Robotics, School of Biomedical Engineering Shanghai Jiao Tong University Shanghai 200240 ChinaInstitute of Medical Robotics, School of Biomedical Engineering Shanghai Jiao Tong University Shanghai 200240 ChinaInstitute of Medical Robotics, School of Biomedical Engineering Shanghai Jiao Tong University Shanghai 200240 ChinaInstitute of Medical Robotics, School of Biomedical Engineering Shanghai Jiao Tong University Shanghai 200240 ChinaInstitute of Medical Robotics, School of Biomedical Engineering Shanghai Jiao Tong University Shanghai 200240 ChinaInstitute of Medical Robotics, School of Biomedical Engineering Shanghai Jiao Tong University Shanghai 200240 ChinaAbstract Tumor‐derived cell‐free DNA (cfDNA) has been exploited as an effective liquid biopsy biomarker for early cancer diagnosis. However, the fragmented and low‐abundance nature in circulating blood pose challenges for highly sensitive cfDNA quantification. Herein, a multifunctional plasmonic biosensor termed Interfacial cfDNA Enrichment, Amplification and Sensing with on‐chip Thermoplasmonics (INEAST) is developed for cfDNA‐based liquid biopsy and lung cancer diagnosis. The INEAST biosensor achieved in situ thermoregulation and label‐free cfDNA biosensing by simultaneously harnessing interfacial thermoplasmonics and localized surface plasmon resonance. Typical cfDNA biomarkers, including epidermal growth factor receptor (EGFR), tumor protein 53 (TP53), phosphatase and tensin homologue deleted on chromosome 10 (PTEN), and cyclin‐dependent kinase inhibitor (CDKN2A), are quantified with detection limits down to femtomolar‐level. Through further validation using blood samples from lung cancer patients, the proposed INEAST bioassays demonstrated superior reliability for lung cancer screening, particularly when combined with clinically available tumor‐protein metrics. This study demonstrated that the INEAST biosensor enables rapid and sensitive cfDNA quantification, yielding a promising and compatible liquid biopsy for early‐stage lung cancer diagnosis.https://doi.org/10.1002/advs.202409708biosensorscell‐free DNAliquid biopsylung cancernucleic acid amplificationthermoplasmonics |
| spellingShingle | Danhua Wang Linlin Liu Wenjing Chi Zhenping Liu Jiayun Wu Yirou Liang Fei He Ruixiang Zhang Pengxin Huang Yunbo Li Guangyu Qiu Interfacial cfDNA Enrichment and Amplification with On‐Chip Thermoplasmonics for Highly Sensitive Cancerous Liquid Biopsy Advanced Science biosensors cell‐free DNA liquid biopsy lung cancer nucleic acid amplification thermoplasmonics |
| title | Interfacial cfDNA Enrichment and Amplification with On‐Chip Thermoplasmonics for Highly Sensitive Cancerous Liquid Biopsy |
| title_full | Interfacial cfDNA Enrichment and Amplification with On‐Chip Thermoplasmonics for Highly Sensitive Cancerous Liquid Biopsy |
| title_fullStr | Interfacial cfDNA Enrichment and Amplification with On‐Chip Thermoplasmonics for Highly Sensitive Cancerous Liquid Biopsy |
| title_full_unstemmed | Interfacial cfDNA Enrichment and Amplification with On‐Chip Thermoplasmonics for Highly Sensitive Cancerous Liquid Biopsy |
| title_short | Interfacial cfDNA Enrichment and Amplification with On‐Chip Thermoplasmonics for Highly Sensitive Cancerous Liquid Biopsy |
| title_sort | interfacial cfdna enrichment and amplification with on chip thermoplasmonics for highly sensitive cancerous liquid biopsy |
| topic | biosensors cell‐free DNA liquid biopsy lung cancer nucleic acid amplification thermoplasmonics |
| url | https://doi.org/10.1002/advs.202409708 |
| work_keys_str_mv | AT danhuawang interfacialcfdnaenrichmentandamplificationwithonchipthermoplasmonicsforhighlysensitivecancerousliquidbiopsy AT linlinliu interfacialcfdnaenrichmentandamplificationwithonchipthermoplasmonicsforhighlysensitivecancerousliquidbiopsy AT wenjingchi interfacialcfdnaenrichmentandamplificationwithonchipthermoplasmonicsforhighlysensitivecancerousliquidbiopsy AT zhenpingliu interfacialcfdnaenrichmentandamplificationwithonchipthermoplasmonicsforhighlysensitivecancerousliquidbiopsy AT jiayunwu interfacialcfdnaenrichmentandamplificationwithonchipthermoplasmonicsforhighlysensitivecancerousliquidbiopsy AT yirouliang interfacialcfdnaenrichmentandamplificationwithonchipthermoplasmonicsforhighlysensitivecancerousliquidbiopsy AT feihe interfacialcfdnaenrichmentandamplificationwithonchipthermoplasmonicsforhighlysensitivecancerousliquidbiopsy AT ruixiangzhang interfacialcfdnaenrichmentandamplificationwithonchipthermoplasmonicsforhighlysensitivecancerousliquidbiopsy AT pengxinhuang interfacialcfdnaenrichmentandamplificationwithonchipthermoplasmonicsforhighlysensitivecancerousliquidbiopsy AT yunboli interfacialcfdnaenrichmentandamplificationwithonchipthermoplasmonicsforhighlysensitivecancerousliquidbiopsy AT guangyuqiu interfacialcfdnaenrichmentandamplificationwithonchipthermoplasmonicsforhighlysensitivecancerousliquidbiopsy |