Pth-carrying high-strength hydrogel promotes bone defect reconstruction via the PTH1R/SLPI pathway
The repair of bone defects, particularly those of a substantial size, has historically posed considerable challenges due to insufficient blood supply and the absence of attachment points. Hydrogels have seen a surge in applications in wound healing and drug delivery due to their exceptional biocompa...
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| Language: | English |
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Elsevier
2025-05-01
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| Series: | Materials & Design |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127525004137 |
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| author | Gang Xiang Feng Zhong Yi Yin Linhua Deng Ang Deng Yunjia Wang Zejiu Tan |
| author_facet | Gang Xiang Feng Zhong Yi Yin Linhua Deng Ang Deng Yunjia Wang Zejiu Tan |
| author_sort | Gang Xiang |
| collection | DOAJ |
| description | The repair of bone defects, particularly those of a substantial size, has historically posed considerable challenges due to insufficient blood supply and the absence of attachment points. Hydrogels have seen a surge in applications in wound healing and drug delivery due to their exceptional biocompatibility and biodegradability. In this study, a high-strength hydrogel (G/mA Gel) was developed to promote bone defect repair by loading parathyroid hormone (PTH). The PTH-G/mA Gel demonstrated the capacity to release PTH in a continuous and stable manner, thereby significantly enhancing the differentiation of MC3T3 osteoblasts. In a rat skull defect model, the bone density and bone volume of the PTH-G/mA gel group were significantly higher than those of the control group and the group using PTH alone. Subsequent analysis revealed that this enhancement was attributable to the slow-release mechanism of PTH, which exerted a synergistic effect on osteogenesis by activating the PTH1R/SLPI pathway in conjunction with the mechanical reinforcement provided by the hydrogel at the defect site. This study proposes a novel, cost-effective strategy with minimal adverse effects, providing a new avenue for the treatment of bone defects. |
| format | Article |
| id | doaj-art-5a7630d8b6904404b15e4790c3580b3f |
| institution | DOAJ |
| issn | 0264-1275 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj-art-5a7630d8b6904404b15e4790c3580b3f2025-08-20T03:22:04ZengElsevierMaterials & Design0264-12752025-05-0125311399310.1016/j.matdes.2025.113993Pth-carrying high-strength hydrogel promotes bone defect reconstruction via the PTH1R/SLPI pathwayGang Xiang0Feng Zhong1Yi Yin2Linhua Deng3Ang Deng4Yunjia Wang5Zejiu Tan6Department of Spine Surgery and Orthopaedics, Xiangya Hospital, Central South University, Changsha 410008 Hunan, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008 Hunan, ChinaPingxiang Jingkai People’s Hospital, Pingxiang 337000 Jiangxi, ChinaNational Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008 Hunan, China; Department of Geriatrics, Respiratory Medicine, Xiangya Hospital, Central South University, Changsha 410008, ChinaDepartment of Spine Surgery and Orthopaedics, Xiangya Hospital, Central South University, Changsha 410008 Hunan, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008 Hunan, ChinaDepartment of Spine Surgery and Orthopaedics, Xiangya Hospital, Central South University, Changsha 410008 Hunan, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008 Hunan, ChinaDepartment of Spine Surgery and Orthopaedics, Xiangya Hospital, Central South University, Changsha 410008 Hunan, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008 Hunan, ChinaDepartment of Spine Surgery and Orthopaedics, Xiangya Hospital, Central South University, Changsha 410008 Hunan, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008 Hunan, China; Corresponding author.The repair of bone defects, particularly those of a substantial size, has historically posed considerable challenges due to insufficient blood supply and the absence of attachment points. Hydrogels have seen a surge in applications in wound healing and drug delivery due to their exceptional biocompatibility and biodegradability. In this study, a high-strength hydrogel (G/mA Gel) was developed to promote bone defect repair by loading parathyroid hormone (PTH). The PTH-G/mA Gel demonstrated the capacity to release PTH in a continuous and stable manner, thereby significantly enhancing the differentiation of MC3T3 osteoblasts. In a rat skull defect model, the bone density and bone volume of the PTH-G/mA gel group were significantly higher than those of the control group and the group using PTH alone. Subsequent analysis revealed that this enhancement was attributable to the slow-release mechanism of PTH, which exerted a synergistic effect on osteogenesis by activating the PTH1R/SLPI pathway in conjunction with the mechanical reinforcement provided by the hydrogel at the defect site. This study proposes a novel, cost-effective strategy with minimal adverse effects, providing a new avenue for the treatment of bone defects.http://www.sciencedirect.com/science/article/pii/S0264127525004137PTHHydrogelBone defect regenerationOsteogenesis |
| spellingShingle | Gang Xiang Feng Zhong Yi Yin Linhua Deng Ang Deng Yunjia Wang Zejiu Tan Pth-carrying high-strength hydrogel promotes bone defect reconstruction via the PTH1R/SLPI pathway Materials & Design PTH Hydrogel Bone defect regeneration Osteogenesis |
| title | Pth-carrying high-strength hydrogel promotes bone defect reconstruction via the PTH1R/SLPI pathway |
| title_full | Pth-carrying high-strength hydrogel promotes bone defect reconstruction via the PTH1R/SLPI pathway |
| title_fullStr | Pth-carrying high-strength hydrogel promotes bone defect reconstruction via the PTH1R/SLPI pathway |
| title_full_unstemmed | Pth-carrying high-strength hydrogel promotes bone defect reconstruction via the PTH1R/SLPI pathway |
| title_short | Pth-carrying high-strength hydrogel promotes bone defect reconstruction via the PTH1R/SLPI pathway |
| title_sort | pth carrying high strength hydrogel promotes bone defect reconstruction via the pth1r slpi pathway |
| topic | PTH Hydrogel Bone defect regeneration Osteogenesis |
| url | http://www.sciencedirect.com/science/article/pii/S0264127525004137 |
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