A causal relationship between type 1 diabetes and risk of systemic sclerosis: a bidirectional two-sample Mendelian randomization study

Abstract Background Previous epidemiological observational studies have indicated an association between type 1 diabetes (T1DM) and systemic sclerosis (SSc). However, the potential causal relationship between T1DM and SSc remains unclear. Therefore, this Mendelian randomization (MR) study aims to in...

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Main Authors: Haochen Huang, Bo Yu, Fengyun Yu, Zhen-zhen Wu, Dong Xu, Ling-ling Zhang
Format: Article
Language:English
Published: BMC 2025-06-01
Series:Diabetology & Metabolic Syndrome
Subjects:
Online Access:https://doi.org/10.1186/s13098-025-01743-3
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author Haochen Huang
Bo Yu
Fengyun Yu
Zhen-zhen Wu
Dong Xu
Ling-ling Zhang
author_facet Haochen Huang
Bo Yu
Fengyun Yu
Zhen-zhen Wu
Dong Xu
Ling-ling Zhang
author_sort Haochen Huang
collection DOAJ
description Abstract Background Previous epidemiological observational studies have indicated an association between type 1 diabetes (T1DM) and systemic sclerosis (SSc). However, the potential causal relationship between T1DM and SSc remains unclear. Therefore, this Mendelian randomization (MR) study aims to investigate the bidirectional causal relationship between T1DM and SSc through a bidirectional two-sample mendelian randomization analysis. Methods We utilized SNP data aggregated from previously published genome-wide association studies (GWAS) and selected appropriate SNPs as instrumental variables (IVs). To explore the bidirectional causal relationship between T1DM and SSc, we conducted bidirectional Mendelian randomization analyses using various methods, including inverse variance weighted (IVW), MR-Egger, weighted median, and weighted mode approaches. Additionally, sensitivity analyses were performed to further validate the accuracy of the MR results. Results Our MR analysis using 66 SNPs as IVs for T1DM showed a genetically determined association with an increased risk of SSc (IVW OR = 1.294, 95% CI 1.140–1.469, p < 0.001), consistent across MR-Egger, weighted median, and weighted mode methods, with no evidence of horizontal pleiotropy or heterogeneity, and robustness confirmed by leave-one-out analysis. Conversely, reverse MR analysis using 7 SNPs for SSc revealed no significant association with T1DM (IVW OR = 0.998, 95% CI 0.979–1.016, P = 0.798), consistent across all methods, with no evidence of pleiotropy or heterogeneity, and results confirmed as robust. Conclusion This pioneering mendelian randomization study reveals a causal link between genetic susceptibility to T1DM and an increased risk of SSc, though not vice versa, emphasizing the need for validation across diverse populations and further exploration of underlying mechanisms.
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spelling doaj-art-5a6f3a6819d34fbeb84ffcd515d693492025-08-20T03:25:19ZengBMCDiabetology & Metabolic Syndrome1758-59962025-06-0117111110.1186/s13098-025-01743-3A causal relationship between type 1 diabetes and risk of systemic sclerosis: a bidirectional two-sample Mendelian randomization studyHaochen Huang0Bo Yu1Fengyun Yu2Zhen-zhen Wu3Dong Xu4Ling-ling Zhang5Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Peking Union Medical CollegeDepartment of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Peking Union Medical CollegeSchool of Engineering Medicine, Beihang UniversityKey Laboratory of Epigenetic Regulation and Intervention, Institute of Biophysics, Chinese Academy of SciencesDepartment of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Peking Union Medical CollegeDepartment of Rheumatology and Clinical Immunology, Beijing Shijitan Hospital, Capital Medical UniversityAbstract Background Previous epidemiological observational studies have indicated an association between type 1 diabetes (T1DM) and systemic sclerosis (SSc). However, the potential causal relationship between T1DM and SSc remains unclear. Therefore, this Mendelian randomization (MR) study aims to investigate the bidirectional causal relationship between T1DM and SSc through a bidirectional two-sample mendelian randomization analysis. Methods We utilized SNP data aggregated from previously published genome-wide association studies (GWAS) and selected appropriate SNPs as instrumental variables (IVs). To explore the bidirectional causal relationship between T1DM and SSc, we conducted bidirectional Mendelian randomization analyses using various methods, including inverse variance weighted (IVW), MR-Egger, weighted median, and weighted mode approaches. Additionally, sensitivity analyses were performed to further validate the accuracy of the MR results. Results Our MR analysis using 66 SNPs as IVs for T1DM showed a genetically determined association with an increased risk of SSc (IVW OR = 1.294, 95% CI 1.140–1.469, p < 0.001), consistent across MR-Egger, weighted median, and weighted mode methods, with no evidence of horizontal pleiotropy or heterogeneity, and robustness confirmed by leave-one-out analysis. Conversely, reverse MR analysis using 7 SNPs for SSc revealed no significant association with T1DM (IVW OR = 0.998, 95% CI 0.979–1.016, P = 0.798), consistent across all methods, with no evidence of pleiotropy or heterogeneity, and results confirmed as robust. Conclusion This pioneering mendelian randomization study reveals a causal link between genetic susceptibility to T1DM and an increased risk of SSc, though not vice versa, emphasizing the need for validation across diverse populations and further exploration of underlying mechanisms.https://doi.org/10.1186/s13098-025-01743-3Autoimmune diseaseMendelian randomizationCausal relationshipType 1 diabetesSystemic sclerosis
spellingShingle Haochen Huang
Bo Yu
Fengyun Yu
Zhen-zhen Wu
Dong Xu
Ling-ling Zhang
A causal relationship between type 1 diabetes and risk of systemic sclerosis: a bidirectional two-sample Mendelian randomization study
Diabetology & Metabolic Syndrome
Autoimmune disease
Mendelian randomization
Causal relationship
Type 1 diabetes
Systemic sclerosis
title A causal relationship between type 1 diabetes and risk of systemic sclerosis: a bidirectional two-sample Mendelian randomization study
title_full A causal relationship between type 1 diabetes and risk of systemic sclerosis: a bidirectional two-sample Mendelian randomization study
title_fullStr A causal relationship between type 1 diabetes and risk of systemic sclerosis: a bidirectional two-sample Mendelian randomization study
title_full_unstemmed A causal relationship between type 1 diabetes and risk of systemic sclerosis: a bidirectional two-sample Mendelian randomization study
title_short A causal relationship between type 1 diabetes and risk of systemic sclerosis: a bidirectional two-sample Mendelian randomization study
title_sort causal relationship between type 1 diabetes and risk of systemic sclerosis a bidirectional two sample mendelian randomization study
topic Autoimmune disease
Mendelian randomization
Causal relationship
Type 1 diabetes
Systemic sclerosis
url https://doi.org/10.1186/s13098-025-01743-3
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