Optimizing the Method for Differentiation of Macrophages from Human Induced Pluripotent Stem Cells
Macrophage is a very promising cell type for cancer immunotherapy, yet it is difficult to obtain enough functional macrophages for clinical cell therapy. Herein, we descibe a reliable method to produce functional macrophages through the differentiation of human induced pluripotent stem cells (hiPSCs...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2022/6593403 |
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| _version_ | 1850225116043018240 |
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| author | Shanshan Li Lili Song Yingwen Zhang Zhiyan Zhan Yi Yang Lisha Yu Hua Zhu Weihua Huang Wanqiao Wang Haizhong Feng Yanxin Li |
| author_facet | Shanshan Li Lili Song Yingwen Zhang Zhiyan Zhan Yi Yang Lisha Yu Hua Zhu Weihua Huang Wanqiao Wang Haizhong Feng Yanxin Li |
| author_sort | Shanshan Li |
| collection | DOAJ |
| description | Macrophage is a very promising cell type for cancer immunotherapy, yet it is difficult to obtain enough functional macrophages for clinical cell therapy. Herein, we descibe a reliable method to produce functional macrophages through the differentiation of human induced pluripotent stem cells (hiPSCs). By optimizing the size control of embryoid bodies (EBs), we accelerated the differentiation process of macrophages and increased the production of macrophages without attenuating macrophage functions. Our final yield of macrophages was close to 50-fold of starting iPSCs. The macrophages showed phagocytic capacity in vitro and a xenograft tumor model. M0 macrophages could be further polarized into M1 and M2 subtypes, and M1 cells exhibited typical proinflammatory characteristics. Moreover, we found that hematopoietic differentiation originated from the outside of EB and matured inward gradually. Taken together, our protocol provides an effective method for the generation of macrophages comparable to blood-derived macrophages, which provides potential value for cell therapy and gene editing studies. |
| format | Article |
| id | doaj-art-5a6dfba7243d4d749f493365e5a7f0e3 |
| institution | OA Journals |
| issn | 1687-9678 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-5a6dfba7243d4d749f493365e5a7f0e32025-08-20T02:05:28ZengWileyStem Cells International1687-96782022-01-01202210.1155/2022/6593403Optimizing the Method for Differentiation of Macrophages from Human Induced Pluripotent Stem CellsShanshan Li0Lili Song1Yingwen Zhang2Zhiyan Zhan3Yi Yang4Lisha Yu5Hua Zhu6Weihua Huang7Wanqiao Wang8Haizhong Feng9Yanxin Li10Pediatric Translational Medicine InstituteDepartment of Hematology & OncologyPediatric Translational Medicine InstitutePediatric Translational Medicine InstitutePediatric Translational Medicine InstituteDepartment of Hematology & OncologyDepartment of Hematology & OncologyDepartment of Transfusion MedicinePediatric Translational Medicine InstituteState Key Laboratory of Oncogenes and Related GenesPediatric Translational Medicine InstituteMacrophage is a very promising cell type for cancer immunotherapy, yet it is difficult to obtain enough functional macrophages for clinical cell therapy. Herein, we descibe a reliable method to produce functional macrophages through the differentiation of human induced pluripotent stem cells (hiPSCs). By optimizing the size control of embryoid bodies (EBs), we accelerated the differentiation process of macrophages and increased the production of macrophages without attenuating macrophage functions. Our final yield of macrophages was close to 50-fold of starting iPSCs. The macrophages showed phagocytic capacity in vitro and a xenograft tumor model. M0 macrophages could be further polarized into M1 and M2 subtypes, and M1 cells exhibited typical proinflammatory characteristics. Moreover, we found that hematopoietic differentiation originated from the outside of EB and matured inward gradually. Taken together, our protocol provides an effective method for the generation of macrophages comparable to blood-derived macrophages, which provides potential value for cell therapy and gene editing studies.http://dx.doi.org/10.1155/2022/6593403 |
| spellingShingle | Shanshan Li Lili Song Yingwen Zhang Zhiyan Zhan Yi Yang Lisha Yu Hua Zhu Weihua Huang Wanqiao Wang Haizhong Feng Yanxin Li Optimizing the Method for Differentiation of Macrophages from Human Induced Pluripotent Stem Cells Stem Cells International |
| title | Optimizing the Method for Differentiation of Macrophages from Human Induced Pluripotent Stem Cells |
| title_full | Optimizing the Method for Differentiation of Macrophages from Human Induced Pluripotent Stem Cells |
| title_fullStr | Optimizing the Method for Differentiation of Macrophages from Human Induced Pluripotent Stem Cells |
| title_full_unstemmed | Optimizing the Method for Differentiation of Macrophages from Human Induced Pluripotent Stem Cells |
| title_short | Optimizing the Method for Differentiation of Macrophages from Human Induced Pluripotent Stem Cells |
| title_sort | optimizing the method for differentiation of macrophages from human induced pluripotent stem cells |
| url | http://dx.doi.org/10.1155/2022/6593403 |
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