Prevalence and molecular characterization of Cryptosporidium spp. in cattle in central and Western Inner Mongolia, China

Cryptosporidium spp. are apicomplexan parasites that can cause diarrhea in humans and animals, posing a health risk to both animals and humans. Molecular epidemiological analysis provides essential data for understanding Cryptosporidium transmission, treatment, and control. In this study, SSU rRNA w...

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Main Authors: Xinlei Yan, Wenhui Guo, Ru Liang, Ruifeng Li, Wenbin Kang
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-07-01
Series:Frontiers in Veterinary Science
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Online Access:https://www.frontiersin.org/articles/10.3389/fvets.2025.1587302/full
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author Xinlei Yan
Wenhui Guo
Ru Liang
Ruifeng Li
Wenbin Kang
author_facet Xinlei Yan
Wenhui Guo
Ru Liang
Ruifeng Li
Wenbin Kang
author_sort Xinlei Yan
collection DOAJ
description Cryptosporidium spp. are apicomplexan parasites that can cause diarrhea in humans and animals, posing a health risk to both animals and humans. Molecular epidemiological analysis provides essential data for understanding Cryptosporidium transmission, treatment, and control. In this study, SSU rRNA was used to determine the prevalence of Cryptosporidium in cattle. A total of 847 fecal samples were collected from 16 farms in central and western Inner Mongolia (Hohhot, Ordos, Bayan Nur and Baotou), and 15.94% (135/847) of samples were positive. Overall, Cryptosporidium was detected in all seasons. Calves up to 2 months of age were found with the highest rate of infection (33.33%). In older animals, there was a significant decline in infection rates with increasing age. The species, C. parvum (n = 105), C. andersoni (n = 21) and C. bovis (n = 9) were detected, individually, or in mixed infections involving two or three Cryptosporidium spp., and five subtypes of C. parvum (IIdA17G1, IIdA17G2, IIdA18G1, IIdA19G1, IIdA20G1) were identified. Our findings provide data to support the epidemiological control of Cryptosporidium infection in cattle.
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spelling doaj-art-5a6de4e05534435f8095d4ce90dc08db2025-08-20T02:41:07ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-07-011210.3389/fvets.2025.15873021587302Prevalence and molecular characterization of Cryptosporidium spp. in cattle in central and Western Inner Mongolia, ChinaXinlei Yan0Wenhui Guo1Ru Liang2Ruifeng Li3Wenbin Kang4Food Science and Engineering College of Inner Mongolia Agricultural University, Hohhot, ChinaFood Science and Engineering College of Inner Mongolia Agricultural University, Hohhot, ChinaDepartment of Pediatrics, Inner Mongolia Maternal and Child Health Care Hospital, Hohhot, ChinaDepartment of Pediatrics, Inner Mongolia Maternal and Child Health Care Hospital, Hohhot, ChinaInner Mongolia Autonomous Region Hospital of Traditional Chinese Medicine, Hohhot, ChinaCryptosporidium spp. are apicomplexan parasites that can cause diarrhea in humans and animals, posing a health risk to both animals and humans. Molecular epidemiological analysis provides essential data for understanding Cryptosporidium transmission, treatment, and control. In this study, SSU rRNA was used to determine the prevalence of Cryptosporidium in cattle. A total of 847 fecal samples were collected from 16 farms in central and western Inner Mongolia (Hohhot, Ordos, Bayan Nur and Baotou), and 15.94% (135/847) of samples were positive. Overall, Cryptosporidium was detected in all seasons. Calves up to 2 months of age were found with the highest rate of infection (33.33%). In older animals, there was a significant decline in infection rates with increasing age. The species, C. parvum (n = 105), C. andersoni (n = 21) and C. bovis (n = 9) were detected, individually, or in mixed infections involving two or three Cryptosporidium spp., and five subtypes of C. parvum (IIdA17G1, IIdA17G2, IIdA18G1, IIdA19G1, IIdA20G1) were identified. Our findings provide data to support the epidemiological control of Cryptosporidium infection in cattle.https://www.frontiersin.org/articles/10.3389/fvets.2025.1587302/fullCryptosporidiumcattlesubtypeprevalencemolecular epidemiological
spellingShingle Xinlei Yan
Wenhui Guo
Ru Liang
Ruifeng Li
Wenbin Kang
Prevalence and molecular characterization of Cryptosporidium spp. in cattle in central and Western Inner Mongolia, China
Frontiers in Veterinary Science
Cryptosporidium
cattle
subtype
prevalence
molecular epidemiological
title Prevalence and molecular characterization of Cryptosporidium spp. in cattle in central and Western Inner Mongolia, China
title_full Prevalence and molecular characterization of Cryptosporidium spp. in cattle in central and Western Inner Mongolia, China
title_fullStr Prevalence and molecular characterization of Cryptosporidium spp. in cattle in central and Western Inner Mongolia, China
title_full_unstemmed Prevalence and molecular characterization of Cryptosporidium spp. in cattle in central and Western Inner Mongolia, China
title_short Prevalence and molecular characterization of Cryptosporidium spp. in cattle in central and Western Inner Mongolia, China
title_sort prevalence and molecular characterization of cryptosporidium spp in cattle in central and western inner mongolia china
topic Cryptosporidium
cattle
subtype
prevalence
molecular epidemiological
url https://www.frontiersin.org/articles/10.3389/fvets.2025.1587302/full
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