Pairing of homologous regions in the mouse genome is associated with transcription but not imprinting status.
Although somatic homologous pairing is common in Drosophila it is not generally observed in mammalian cells. However, a number of regions have recently been shown to come into close proximity with their homologous allele, and it has been proposed that pairing might be involved in the establishment o...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0038983&type=printable |
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| _version_ | 1849470491771797504 |
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| author | Christel Krueger Michelle R King Felix Krueger Miguel R Branco Cameron S Osborne Kathy K Niakan Michael J Higgins Wolf Reik |
| author_facet | Christel Krueger Michelle R King Felix Krueger Miguel R Branco Cameron S Osborne Kathy K Niakan Michael J Higgins Wolf Reik |
| author_sort | Christel Krueger |
| collection | DOAJ |
| description | Although somatic homologous pairing is common in Drosophila it is not generally observed in mammalian cells. However, a number of regions have recently been shown to come into close proximity with their homologous allele, and it has been proposed that pairing might be involved in the establishment or maintenance of monoallelic expression. Here, we investigate the pairing properties of various imprinted and non-imprinted regions in mouse tissues and ES cells. We find by allele-specific 4C-Seq and DNA FISH that the Kcnq1 imprinted region displays frequent pairing but that this is not dependent on monoallelic expression. We demonstrate that pairing involves larger chromosomal regions and that the two chromosome territories come close together. Frequent pairing is not associated with imprinted status or DNA repair, but is influenced by chromosomal location and transcription. We propose that homologous pairing is not exclusive to specialised regions or specific functional events, and speculate that it provides the cell with the opportunity of trans-allelic effects on gene regulation. |
| format | Article |
| id | doaj-art-5a6cb25828564866ad2bf47bfb7b4369 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-5a6cb25828564866ad2bf47bfb7b43692025-08-20T03:25:08ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0177e3898310.1371/journal.pone.0038983Pairing of homologous regions in the mouse genome is associated with transcription but not imprinting status.Christel KruegerMichelle R KingFelix KruegerMiguel R BrancoCameron S OsborneKathy K NiakanMichael J HigginsWolf ReikAlthough somatic homologous pairing is common in Drosophila it is not generally observed in mammalian cells. However, a number of regions have recently been shown to come into close proximity with their homologous allele, and it has been proposed that pairing might be involved in the establishment or maintenance of monoallelic expression. Here, we investigate the pairing properties of various imprinted and non-imprinted regions in mouse tissues and ES cells. We find by allele-specific 4C-Seq and DNA FISH that the Kcnq1 imprinted region displays frequent pairing but that this is not dependent on monoallelic expression. We demonstrate that pairing involves larger chromosomal regions and that the two chromosome territories come close together. Frequent pairing is not associated with imprinted status or DNA repair, but is influenced by chromosomal location and transcription. We propose that homologous pairing is not exclusive to specialised regions or specific functional events, and speculate that it provides the cell with the opportunity of trans-allelic effects on gene regulation.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0038983&type=printable |
| spellingShingle | Christel Krueger Michelle R King Felix Krueger Miguel R Branco Cameron S Osborne Kathy K Niakan Michael J Higgins Wolf Reik Pairing of homologous regions in the mouse genome is associated with transcription but not imprinting status. PLoS ONE |
| title | Pairing of homologous regions in the mouse genome is associated with transcription but not imprinting status. |
| title_full | Pairing of homologous regions in the mouse genome is associated with transcription but not imprinting status. |
| title_fullStr | Pairing of homologous regions in the mouse genome is associated with transcription but not imprinting status. |
| title_full_unstemmed | Pairing of homologous regions in the mouse genome is associated with transcription but not imprinting status. |
| title_short | Pairing of homologous regions in the mouse genome is associated with transcription but not imprinting status. |
| title_sort | pairing of homologous regions in the mouse genome is associated with transcription but not imprinting status |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0038983&type=printable |
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