Comparing Two Seawater Temperatures For Human Norovirus Depuration From Oysters
Despite regulations set up to monitor the microbiological quality of shellfish in producing areas, shellfish-borne gastroenteritis outbreaks still occur. Indeed, oyster depuration practices that are efficient to eliminate bacteria, fail to eliminate human norovirus from oyster flesh. In order to eva...
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Elsevier
2025-01-01
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| Series: | Journal of Food Protection |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0362028X2400190X |
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| author | Françoise S. Le Guyader Joanna Ollivier Sylvain Parnaudeau Mathias Gauffriau Mathias Papin Christophe Stavrakakis Virginie François Françoise Vincent-Hubert Pascal Garry |
| author_facet | Françoise S. Le Guyader Joanna Ollivier Sylvain Parnaudeau Mathias Gauffriau Mathias Papin Christophe Stavrakakis Virginie François Françoise Vincent-Hubert Pascal Garry |
| author_sort | Françoise S. Le Guyader |
| collection | DOAJ |
| description | Despite regulations set up to monitor the microbiological quality of shellfish in producing areas, shellfish-borne gastroenteritis outbreaks still occur. Indeed, oyster depuration practices that are efficient to eliminate bacteria, fail to eliminate human norovirus from oyster flesh. In order to evaluate the impact of seawater temperature on the elimination of norovirus particles from oysters, large batches of oysters were contaminated using raw sewage containing norovirus and subjected to depuration at 8 °C or 18 °C. Over the experiment, quantitative RT-qPCR showed a one-log decrease of norovirus (both genogroups combined) genome copies per gram of digestive tissue after 41 days for oysters depurated at 8 °C and 24 days at 18 °C. The decrease of norovirus (both genogroups combined) in two batches of field-contaminated oysters depurated for two weeks at 18 °C was in the same range (21 and 23 days, respectively). All experiments showed a difference in genomic decay between the two norovirus genogroups, with norovirus genogroup I being more persistent in March/April compared to April/May. |
| format | Article |
| id | doaj-art-5a6196bc24574ce7a69e3542457e8881 |
| institution | Kabale University |
| issn | 0362-028X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Food Protection |
| spelling | doaj-art-5a6196bc24574ce7a69e3542457e88812025-01-09T06:12:31ZengElsevierJournal of Food Protection0362-028X2025-01-01881100406Comparing Two Seawater Temperatures For Human Norovirus Depuration From OystersFrançoise S. Le Guyader0Joanna Ollivier1Sylvain Parnaudeau2Mathias Gauffriau3Mathias Papin4Christophe Stavrakakis5Virginie François6Françoise Vincent-Hubert7Pascal Garry8Ifremer, U. Microbiologie Aliment Santé Environnement, LSEM/RBE, Nantes, France; Corresponding author at: Ifremer, Laboratoire de Microbiologie, BP 21105, 44311 Nantes cedex 03, France.Ifremer, U. Microbiologie Aliment Santé Environnement, LSEM/RBE, Nantes, FranceIfremer, U. Microbiologie Aliment Santé Environnement, LSEM/RBE, Nantes, FranceIfremer, U. Microbiologie Aliment Santé Environnement, LSEM/RBE, Nantes, FranceIfremer, U. EMMA Experimentale Mollusques Marins Atlantique, PMMB/RBE, Bouin, FranceIfremer, U. EMMA Experimentale Mollusques Marins Atlantique, PMMB/RBE, Bouin, FranceIfremer, U. EMMA Experimentale Mollusques Marins Atlantique, PMMB/RBE, Bouin, FranceIfremer, U. Microbiologie Aliment Santé Environnement, LSEM/RBE, Nantes, FranceIfremer, U. Microbiologie Aliment Santé Environnement, LSEM/RBE, Nantes, FranceDespite regulations set up to monitor the microbiological quality of shellfish in producing areas, shellfish-borne gastroenteritis outbreaks still occur. Indeed, oyster depuration practices that are efficient to eliminate bacteria, fail to eliminate human norovirus from oyster flesh. In order to evaluate the impact of seawater temperature on the elimination of norovirus particles from oysters, large batches of oysters were contaminated using raw sewage containing norovirus and subjected to depuration at 8 °C or 18 °C. Over the experiment, quantitative RT-qPCR showed a one-log decrease of norovirus (both genogroups combined) genome copies per gram of digestive tissue after 41 days for oysters depurated at 8 °C and 24 days at 18 °C. The decrease of norovirus (both genogroups combined) in two batches of field-contaminated oysters depurated for two weeks at 18 °C was in the same range (21 and 23 days, respectively). All experiments showed a difference in genomic decay between the two norovirus genogroups, with norovirus genogroup I being more persistent in March/April compared to April/May.http://www.sciencedirect.com/science/article/pii/S0362028X2400190XDepurationField-contaminated oystersHuman norovirusOystersShellfishT90 |
| spellingShingle | Françoise S. Le Guyader Joanna Ollivier Sylvain Parnaudeau Mathias Gauffriau Mathias Papin Christophe Stavrakakis Virginie François Françoise Vincent-Hubert Pascal Garry Comparing Two Seawater Temperatures For Human Norovirus Depuration From Oysters Journal of Food Protection Depuration Field-contaminated oysters Human norovirus Oysters Shellfish T90 |
| title | Comparing Two Seawater Temperatures For Human Norovirus Depuration From Oysters |
| title_full | Comparing Two Seawater Temperatures For Human Norovirus Depuration From Oysters |
| title_fullStr | Comparing Two Seawater Temperatures For Human Norovirus Depuration From Oysters |
| title_full_unstemmed | Comparing Two Seawater Temperatures For Human Norovirus Depuration From Oysters |
| title_short | Comparing Two Seawater Temperatures For Human Norovirus Depuration From Oysters |
| title_sort | comparing two seawater temperatures for human norovirus depuration from oysters |
| topic | Depuration Field-contaminated oysters Human norovirus Oysters Shellfish T90 |
| url | http://www.sciencedirect.com/science/article/pii/S0362028X2400190X |
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