Extraction of walnut protein using deep eutectic solvent aqueous two-phase system: Investigation of structural and functional properties
Walnut protein (WP) exhibits high nutritional value and excellent techno-functional properties, making it a promising candidate for broad applications in the food industry. It is commonly extracted from the meal after oil processing of walnut through alkali extraction with isoelectric precipitation...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-05-01
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| Series: | Food Chemistry: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157525003931 |
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| author | Yun-Kun Zhu Mei-Hui Li Min Yang Jiang-Xia Xu Meng-Yao Ren Zhong-Kai Zhao Liang Wang Jie Yang Jun Liu Min-Wei Zhang |
| author_facet | Yun-Kun Zhu Mei-Hui Li Min Yang Jiang-Xia Xu Meng-Yao Ren Zhong-Kai Zhao Liang Wang Jie Yang Jun Liu Min-Wei Zhang |
| author_sort | Yun-Kun Zhu |
| collection | DOAJ |
| description | Walnut protein (WP) exhibits high nutritional value and excellent techno-functional properties, making it a promising candidate for broad applications in the food industry. It is commonly extracted from the meal after oil processing of walnut through alkali extraction with isoelectric precipitation (AE-IP). In this study, an aqueous two-phase system (DES-ATPS) consisting of betaine-urea/K2HPO4 was used for the extraction of WP from walnut kernels and cold-pressed walnut meal. Comparison with AE-IP, WP extracted through DES-ATPS exhibited enhanced color characteristics and there was no significant difference in protein subunit composition. The WP extracted by DES-ATPS displayed lower Surface hydrophobicity (H0) and intrinsic fluorescence intensities. Conversely, the exposure of internal groups in WP extracted by AE-IP resulted in a higher Zeta potential and a decrease in protein particle size. The comparative analysis revealed fundamentally distinct structural impacts between the two extraction methodologies. Notably, DES-ATPS-isolated WP demonstrated superior solubility under acidic conditions (pH 2). |
| format | Article |
| id | doaj-art-5a4e57b5cb1241009c7351f8e1c571e3 |
| institution | OA Journals |
| issn | 2590-1575 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-5a4e57b5cb1241009c7351f8e1c571e32025-08-20T02:31:54ZengElsevierFood Chemistry: X2590-15752025-05-012810254610.1016/j.fochx.2025.102546Extraction of walnut protein using deep eutectic solvent aqueous two-phase system: Investigation of structural and functional propertiesYun-Kun Zhu0Mei-Hui Li1Min Yang2Jiang-Xia Xu3Meng-Yao Ren4Zhong-Kai Zhao5Liang Wang6Jie Yang7Jun Liu8Min-Wei Zhang9Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaXinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, No.17 Qinghua Donglu, Haidian District, Beijing 100083, China; Corresponding authors.Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830017, China; Corresponding authors.Walnut protein (WP) exhibits high nutritional value and excellent techno-functional properties, making it a promising candidate for broad applications in the food industry. It is commonly extracted from the meal after oil processing of walnut through alkali extraction with isoelectric precipitation (AE-IP). In this study, an aqueous two-phase system (DES-ATPS) consisting of betaine-urea/K2HPO4 was used for the extraction of WP from walnut kernels and cold-pressed walnut meal. Comparison with AE-IP, WP extracted through DES-ATPS exhibited enhanced color characteristics and there was no significant difference in protein subunit composition. The WP extracted by DES-ATPS displayed lower Surface hydrophobicity (H0) and intrinsic fluorescence intensities. Conversely, the exposure of internal groups in WP extracted by AE-IP resulted in a higher Zeta potential and a decrease in protein particle size. The comparative analysis revealed fundamentally distinct structural impacts between the two extraction methodologies. Notably, DES-ATPS-isolated WP demonstrated superior solubility under acidic conditions (pH 2).http://www.sciencedirect.com/science/article/pii/S2590157525003931Walnut proteinDeep eutectic solventsProtein extractionStructural propertiesFunctional properties |
| spellingShingle | Yun-Kun Zhu Mei-Hui Li Min Yang Jiang-Xia Xu Meng-Yao Ren Zhong-Kai Zhao Liang Wang Jie Yang Jun Liu Min-Wei Zhang Extraction of walnut protein using deep eutectic solvent aqueous two-phase system: Investigation of structural and functional properties Food Chemistry: X Walnut protein Deep eutectic solvents Protein extraction Structural properties Functional properties |
| title | Extraction of walnut protein using deep eutectic solvent aqueous two-phase system: Investigation of structural and functional properties |
| title_full | Extraction of walnut protein using deep eutectic solvent aqueous two-phase system: Investigation of structural and functional properties |
| title_fullStr | Extraction of walnut protein using deep eutectic solvent aqueous two-phase system: Investigation of structural and functional properties |
| title_full_unstemmed | Extraction of walnut protein using deep eutectic solvent aqueous two-phase system: Investigation of structural and functional properties |
| title_short | Extraction of walnut protein using deep eutectic solvent aqueous two-phase system: Investigation of structural and functional properties |
| title_sort | extraction of walnut protein using deep eutectic solvent aqueous two phase system investigation of structural and functional properties |
| topic | Walnut protein Deep eutectic solvents Protein extraction Structural properties Functional properties |
| url | http://www.sciencedirect.com/science/article/pii/S2590157525003931 |
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