Simple Spectrophotometric Methods for the Determination of Sulfamethaxazole in Pharmaceuticals Using Folinciocalteau and Orcinol as Reagents
Two simple, sensitive and reproducible spectrophotometric methods (Method A and Method B) were developed for the determination of sulfamethaxazole in bulk and in dosage forms. Method A is based reduction of phosphomolybdic acid present in Folin Ciocalteau reagent by the drug sulfamethaxazole in the...
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2009/457130 |
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| author | G. Vijaya Raja C. Bala Sekaran D. Winnie Teja B. Madhuri B. Jayasree |
| author_facet | G. Vijaya Raja C. Bala Sekaran D. Winnie Teja B. Madhuri B. Jayasree |
| author_sort | G. Vijaya Raja |
| collection | DOAJ |
| description | Two simple, sensitive and reproducible spectrophotometric methods (Method A and Method B) were developed for the determination of sulfamethaxazole in bulk and in dosage forms. Method A is based reduction of phosphomolybdic acid present in Folin Ciocalteau reagent by the drug sulfamethaxazole in the presence of sodium carbonate to form a blue colored chromogen having maximum absorption at 760 nm. Method B is based on the diazotization of the drug by sodium nitrite in acidic medium at 5 °C followed by coupling with orcinol to form yellow colored species (λmax 390 nm). Beer’s law is obeyed in the range of 5-25 μg/mL for method A and 2-10 μg/mL for method B. Results of analysis were validated statistically and by recovery studies. These methods are successfully employed for the determination of sulfamethaxazole in various pharmaceutical preparations and biological fluids. |
| format | Article |
| id | doaj-art-5a4c753d808b409393e44db5096513a9 |
| institution | OA Journals |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-5a4c753d808b409393e44db5096513a92025-08-20T02:05:29ZengWileyE-Journal of Chemistry0973-49452090-98102009-01-016235736010.1155/2009/457130Simple Spectrophotometric Methods for the Determination of Sulfamethaxazole in Pharmaceuticals Using Folinciocalteau and Orcinol as ReagentsG. Vijaya Raja0C. Bala Sekaran1D. Winnie Teja2B. Madhuri3B. Jayasree4Department of Biotechnology, Acharya Nagarjuna University, Guntur, IndiaDepartment of Biotechnology, P. B. Siddhartha College of Arts & Science, Vijayawada – 520 010, IndiaDepartment of Biotechnology, P. B. Siddhartha College of Arts & Science, Vijayawada – 520 010, IndiaDepartment of Biotechnology, P. B. Siddhartha College of Arts & Science, Vijayawada – 520 010, IndiaDepartment of Biotechnology, P. B. Siddhartha College of Arts & Science, Vijayawada – 520 010, IndiaTwo simple, sensitive and reproducible spectrophotometric methods (Method A and Method B) were developed for the determination of sulfamethaxazole in bulk and in dosage forms. Method A is based reduction of phosphomolybdic acid present in Folin Ciocalteau reagent by the drug sulfamethaxazole in the presence of sodium carbonate to form a blue colored chromogen having maximum absorption at 760 nm. Method B is based on the diazotization of the drug by sodium nitrite in acidic medium at 5 °C followed by coupling with orcinol to form yellow colored species (λmax 390 nm). Beer’s law is obeyed in the range of 5-25 μg/mL for method A and 2-10 μg/mL for method B. Results of analysis were validated statistically and by recovery studies. These methods are successfully employed for the determination of sulfamethaxazole in various pharmaceutical preparations and biological fluids.http://dx.doi.org/10.1155/2009/457130 |
| spellingShingle | G. Vijaya Raja C. Bala Sekaran D. Winnie Teja B. Madhuri B. Jayasree Simple Spectrophotometric Methods for the Determination of Sulfamethaxazole in Pharmaceuticals Using Folinciocalteau and Orcinol as Reagents E-Journal of Chemistry |
| title | Simple Spectrophotometric Methods for the Determination of Sulfamethaxazole in Pharmaceuticals Using Folinciocalteau and Orcinol as Reagents |
| title_full | Simple Spectrophotometric Methods for the Determination of Sulfamethaxazole in Pharmaceuticals Using Folinciocalteau and Orcinol as Reagents |
| title_fullStr | Simple Spectrophotometric Methods for the Determination of Sulfamethaxazole in Pharmaceuticals Using Folinciocalteau and Orcinol as Reagents |
| title_full_unstemmed | Simple Spectrophotometric Methods for the Determination of Sulfamethaxazole in Pharmaceuticals Using Folinciocalteau and Orcinol as Reagents |
| title_short | Simple Spectrophotometric Methods for the Determination of Sulfamethaxazole in Pharmaceuticals Using Folinciocalteau and Orcinol as Reagents |
| title_sort | simple spectrophotometric methods for the determination of sulfamethaxazole in pharmaceuticals using folinciocalteau and orcinol as reagents |
| url | http://dx.doi.org/10.1155/2009/457130 |
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