Isolation and partial chemical characterization of macrophage-derived neutrophil chemotactic factor
Macrophages stimulated with lipopolysaccharide (LPS) release a factor (MNCF; macrophage-derived neutrophil chemotactic factor) which induces neutrophil migration in vivo and in vitro. The in vivo chemotactic activity of crude MNCF is not affected by pretreating the animals with dexamethasone, an unc...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
1995-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S096293519500041X |
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| author | M. Dias-Baruffi M. C. Roque-Barreira F. Q. Cunha S. H. Ferreira |
| author_facet | M. Dias-Baruffi M. C. Roque-Barreira F. Q. Cunha S. H. Ferreira |
| author_sort | M. Dias-Baruffi |
| collection | DOAJ |
| description | Macrophages stimulated with lipopolysaccharide (LPS) release a factor (MNCF; macrophage-derived neutrophil chemotactic factor) which induces neutrophil migration in vivo and in vitro. The in vivo chemotactic activity of crude MNCF is not affected by pretreating the animals with dexamethasone, an uncommon characteristic which discriminates MNCF from known chemotactic cytokines. We purified MNCF by affinity chromatography of the supernatant from LPS-stimulated macrophages on immobilized D-galactose, followed by gel filtration of the sugar-binding material on Superdex 75. The activity was eluted in the volume corresponding to a MW of 54 kDa. SDS–PAGE of this preparation revealed a single band, also corresponding to a 54 kDa protein. MNCF is an acidic protein (pI < 4) as shown by chromatofocussing. Like the crude MNCF, the homogeneous protein induced neutrophil migration in vitro as well as in vivo. This was not modified by dexamethasone pretreatment. |
| format | Article |
| id | doaj-art-5a44a200ac8144db8b8635c9fa42c9ab |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1995-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-5a44a200ac8144db8b8635c9fa42c9ab2025-08-20T02:05:28ZengWileyMediators of Inflammation0962-93511466-18611995-01-014425726210.1155/S096293519500041XIsolation and partial chemical characterization of macrophage-derived neutrophil chemotactic factorM. Dias-Baruffi0M. C. Roque-Barreira1F. Q. Cunha2S. H. Ferreira3Department of Immunology, Faculty of Medicine, São Paulo, 14, Ribeirão Preto 049-900, BrazilDepartment of Immunology, Faculty of Medicine, São Paulo, 14, Ribeirão Preto 049-900, BrazilDepartment of pharmacology, Faculty of Medicine, São Paulo, 14, Ribeirão Preto 049-900, BrazilDepartment of pharmacology, Faculty of Medicine, São Paulo, 14, Ribeirão Preto 049-900, BrazilMacrophages stimulated with lipopolysaccharide (LPS) release a factor (MNCF; macrophage-derived neutrophil chemotactic factor) which induces neutrophil migration in vivo and in vitro. The in vivo chemotactic activity of crude MNCF is not affected by pretreating the animals with dexamethasone, an uncommon characteristic which discriminates MNCF from known chemotactic cytokines. We purified MNCF by affinity chromatography of the supernatant from LPS-stimulated macrophages on immobilized D-galactose, followed by gel filtration of the sugar-binding material on Superdex 75. The activity was eluted in the volume corresponding to a MW of 54 kDa. SDS–PAGE of this preparation revealed a single band, also corresponding to a 54 kDa protein. MNCF is an acidic protein (pI < 4) as shown by chromatofocussing. Like the crude MNCF, the homogeneous protein induced neutrophil migration in vitro as well as in vivo. This was not modified by dexamethasone pretreatment.http://dx.doi.org/10.1155/S096293519500041X |
| spellingShingle | M. Dias-Baruffi M. C. Roque-Barreira F. Q. Cunha S. H. Ferreira Isolation and partial chemical characterization of macrophage-derived neutrophil chemotactic factor Mediators of Inflammation |
| title | Isolation and partial chemical characterization of macrophage-derived neutrophil chemotactic factor |
| title_full | Isolation and partial chemical characterization of macrophage-derived neutrophil chemotactic factor |
| title_fullStr | Isolation and partial chemical characterization of macrophage-derived neutrophil chemotactic factor |
| title_full_unstemmed | Isolation and partial chemical characterization of macrophage-derived neutrophil chemotactic factor |
| title_short | Isolation and partial chemical characterization of macrophage-derived neutrophil chemotactic factor |
| title_sort | isolation and partial chemical characterization of macrophage derived neutrophil chemotactic factor |
| url | http://dx.doi.org/10.1155/S096293519500041X |
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