Screening and Selection of Native Lactic Acid Bacteria Isolated from Chilean Grapes
The aim of this study was investigating the biological diversity of lactic acid bacteria isolated from Chilean grapes and identifying potential candidates for use as malolactic fermentation starter cultures. The isolated bacteria underwent a comprehensive six-stage screening process, which was mutua...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/14/1/143 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841549140374323200 |
|---|---|
| author | Carla Vargas-Luna Liliana Godoy Sergio Benavides Consuelo Ceppi de Lecco Alejandra Urtubia Wendy Franco |
| author_facet | Carla Vargas-Luna Liliana Godoy Sergio Benavides Consuelo Ceppi de Lecco Alejandra Urtubia Wendy Franco |
| author_sort | Carla Vargas-Luna |
| collection | DOAJ |
| description | The aim of this study was investigating the biological diversity of lactic acid bacteria isolated from Chilean grapes and identifying potential candidates for use as malolactic fermentation starter cultures. The isolated bacteria underwent a comprehensive six-stage screening process, which was mutually exclusive except for the evaluation of tyramine production and citric acid intake. This process included morphological, metabolic, fermentation yield, and resistance tests to identify promising malolactic strains. Morphological assessments led to the selection of 23 isolates, which were genetically identified as <i>Levilactobacillus brevis</i> (65% abundance) and <i>Leuconostoc mesenteroides</i> (35% abundance). Among these, eight strains exhibited low sugar metabolism rates, while three demonstrated competitive growth and malolactic activity in a synthetic medium containing 10% ethanol, outperforming a commercial strain. Low consumption was observed in the qualitative citric acid intake test, whereas a positive response was noted for tyramine production. At the conclusion of the sequential selection criteria, <i>Levilactobacillus brevis</i> BCV-46 exhibited the most favorable characteristics for potential use as a malolactic starter culture, successfully withstanding the combined stress factors of ethanol, pH, and SO<sub>2</sub>. |
| format | Article |
| id | doaj-art-5a32a7dc957c438199727411e31a0a14 |
| institution | Kabale University |
| issn | 2304-8158 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj-art-5a32a7dc957c438199727411e31a0a142025-01-10T13:17:55ZengMDPI AGFoods2304-81582025-01-0114114310.3390/foods14010143Screening and Selection of Native Lactic Acid Bacteria Isolated from Chilean GrapesCarla Vargas-Luna0Liliana Godoy1Sergio Benavides2Consuelo Ceppi de Lecco3Alejandra Urtubia4Wendy Franco5Departamento de Ingeniería Química y Bioprocesos, Pontificia Universidad Católica de Chile, Santiago 6904411, ChileDepartamento de Fruticultura y Enología, Facultad de Agronomía y Sistemas Naturales, Pontificia Universidad Católica de Chile, Santiago 6904411, ChileCarrera de Nutrición y Dietética, Escuela de Nutrición y Dietética, Facultad de Ciencias para el Cuidado de la Salud, Universidad San Sebastina, Campus Las Tres Pascualas, Lientur 1457, Concepción 4080871, ChileDepartamento de Fruticultura y Enología, Facultad de Agronomía y Sistemas Naturales, Pontificia Universidad Católica de Chile, Santiago 6904411, ChileDepartamento de Ingeniería Química Medio Ambiental, Universidad Técnico Federico Santa María, Av. 12 España 1680, Valparaíso 2390123, ChileDepartamento de Ingeniería Química y Bioprocesos, Pontificia Universidad Católica de Chile, Santiago 6904411, ChileThe aim of this study was investigating the biological diversity of lactic acid bacteria isolated from Chilean grapes and identifying potential candidates for use as malolactic fermentation starter cultures. The isolated bacteria underwent a comprehensive six-stage screening process, which was mutually exclusive except for the evaluation of tyramine production and citric acid intake. This process included morphological, metabolic, fermentation yield, and resistance tests to identify promising malolactic strains. Morphological assessments led to the selection of 23 isolates, which were genetically identified as <i>Levilactobacillus brevis</i> (65% abundance) and <i>Leuconostoc mesenteroides</i> (35% abundance). Among these, eight strains exhibited low sugar metabolism rates, while three demonstrated competitive growth and malolactic activity in a synthetic medium containing 10% ethanol, outperforming a commercial strain. Low consumption was observed in the qualitative citric acid intake test, whereas a positive response was noted for tyramine production. At the conclusion of the sequential selection criteria, <i>Levilactobacillus brevis</i> BCV-46 exhibited the most favorable characteristics for potential use as a malolactic starter culture, successfully withstanding the combined stress factors of ethanol, pH, and SO<sub>2</sub>.https://www.mdpi.com/2304-8158/14/1/143native bacteriastarter culture<i>Levilactobacillus brevis</i>malolactic fermentation |
| spellingShingle | Carla Vargas-Luna Liliana Godoy Sergio Benavides Consuelo Ceppi de Lecco Alejandra Urtubia Wendy Franco Screening and Selection of Native Lactic Acid Bacteria Isolated from Chilean Grapes Foods native bacteria starter culture <i>Levilactobacillus brevis</i> malolactic fermentation |
| title | Screening and Selection of Native Lactic Acid Bacteria Isolated from Chilean Grapes |
| title_full | Screening and Selection of Native Lactic Acid Bacteria Isolated from Chilean Grapes |
| title_fullStr | Screening and Selection of Native Lactic Acid Bacteria Isolated from Chilean Grapes |
| title_full_unstemmed | Screening and Selection of Native Lactic Acid Bacteria Isolated from Chilean Grapes |
| title_short | Screening and Selection of Native Lactic Acid Bacteria Isolated from Chilean Grapes |
| title_sort | screening and selection of native lactic acid bacteria isolated from chilean grapes |
| topic | native bacteria starter culture <i>Levilactobacillus brevis</i> malolactic fermentation |
| url | https://www.mdpi.com/2304-8158/14/1/143 |
| work_keys_str_mv | AT carlavargasluna screeningandselectionofnativelacticacidbacteriaisolatedfromchileangrapes AT lilianagodoy screeningandselectionofnativelacticacidbacteriaisolatedfromchileangrapes AT sergiobenavides screeningandselectionofnativelacticacidbacteriaisolatedfromchileangrapes AT consueloceppidelecco screeningandselectionofnativelacticacidbacteriaisolatedfromchileangrapes AT alejandraurtubia screeningandselectionofnativelacticacidbacteriaisolatedfromchileangrapes AT wendyfranco screeningandselectionofnativelacticacidbacteriaisolatedfromchileangrapes |