Dietary 5-Aminolevulinic Acid Alleviates Heat Stress-Induced Renal Injury in Laying Hens by Improving Mitochondrial Quality and Enhancing Antioxidant Activity
This study aimed to evaluate the effects of dietary 5-aminolevulinic acid (ALA) on laying hens to alleviate chronic heat stress-induced renal damage, resulting in improved egg productivity and eggshell quality. A total of 57 white-leghorn laying hens (46 weeks old) were randomly assigned to three gr...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-05-01
|
| Series: | Antioxidants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3921/14/5/556 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849711909813616640 |
|---|---|
| author | Fumika Nanto-Hara Haruhiko Ohtsu |
| author_facet | Fumika Nanto-Hara Haruhiko Ohtsu |
| author_sort | Fumika Nanto-Hara |
| collection | DOAJ |
| description | This study aimed to evaluate the effects of dietary 5-aminolevulinic acid (ALA) on laying hens to alleviate chronic heat stress-induced renal damage, resulting in improved egg productivity and eggshell quality. A total of 57 white-leghorn laying hens (46 weeks old) were randomly assigned to three groups and fed three experimental diets with different levels of ALA (0, 10, and 100 ppm) for 1 week. The birds in each group were then divided into two subgroups; one of the two subgroups was subjected to heat stress (33 °C for 3 weeks), whereas the other group was maintained at 24 °C. Heat exposure significantly decreased the laying rate and eggshell strength and caused renal damage, whereas ALA supplementation alleviated heat-induced poor productivity and renal damage. ALA increased the renal mitochondrial DNA copy number and downregulated the expression of the cGAS-STING pathway-related genes in the kidneys of heat-stressed hens. Furthermore, ALA upregulated the renal expression levels of <i>NRF2</i> and <i>HO-1</i>, whereas it downregulated those of <i>NF-κB</i> and tended to decrease the content of TBARS in the kidney (<i>p</i> = 0.07). Dietary ALA confers a renal protective effect by reducing heat-induced mitochondrial damage and enhancing antioxidant activity, which may contribute to improved productivity under chronic heat stress. |
| format | Article |
| id | doaj-art-5a2d9d9ebb6848cf9e230bc301d75f08 |
| institution | DOAJ |
| issn | 2076-3921 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj-art-5a2d9d9ebb6848cf9e230bc301d75f082025-08-20T03:14:29ZengMDPI AGAntioxidants2076-39212025-05-0114555610.3390/antiox14050556Dietary 5-Aminolevulinic Acid Alleviates Heat Stress-Induced Renal Injury in Laying Hens by Improving Mitochondrial Quality and Enhancing Antioxidant ActivityFumika Nanto-Hara0Haruhiko Ohtsu1Division of Meat Animal and Poultry Research, Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization (NILGS), Tsukuba 305-0901, JapanDepartment of Research Promotion, Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization (NILGS), Tsukuba 305-0901, JapanThis study aimed to evaluate the effects of dietary 5-aminolevulinic acid (ALA) on laying hens to alleviate chronic heat stress-induced renal damage, resulting in improved egg productivity and eggshell quality. A total of 57 white-leghorn laying hens (46 weeks old) were randomly assigned to three groups and fed three experimental diets with different levels of ALA (0, 10, and 100 ppm) for 1 week. The birds in each group were then divided into two subgroups; one of the two subgroups was subjected to heat stress (33 °C for 3 weeks), whereas the other group was maintained at 24 °C. Heat exposure significantly decreased the laying rate and eggshell strength and caused renal damage, whereas ALA supplementation alleviated heat-induced poor productivity and renal damage. ALA increased the renal mitochondrial DNA copy number and downregulated the expression of the cGAS-STING pathway-related genes in the kidneys of heat-stressed hens. Furthermore, ALA upregulated the renal expression levels of <i>NRF2</i> and <i>HO-1</i>, whereas it downregulated those of <i>NF-κB</i> and tended to decrease the content of TBARS in the kidney (<i>p</i> = 0.07). Dietary ALA confers a renal protective effect by reducing heat-induced mitochondrial damage and enhancing antioxidant activity, which may contribute to improved productivity under chronic heat stress.https://www.mdpi.com/2076-3921/14/5/556cGAS-STINGheat stressheme oxygenase-1laying hensmitochondriarenal damage |
| spellingShingle | Fumika Nanto-Hara Haruhiko Ohtsu Dietary 5-Aminolevulinic Acid Alleviates Heat Stress-Induced Renal Injury in Laying Hens by Improving Mitochondrial Quality and Enhancing Antioxidant Activity Antioxidants cGAS-STING heat stress heme oxygenase-1 laying hens mitochondria renal damage |
| title | Dietary 5-Aminolevulinic Acid Alleviates Heat Stress-Induced Renal Injury in Laying Hens by Improving Mitochondrial Quality and Enhancing Antioxidant Activity |
| title_full | Dietary 5-Aminolevulinic Acid Alleviates Heat Stress-Induced Renal Injury in Laying Hens by Improving Mitochondrial Quality and Enhancing Antioxidant Activity |
| title_fullStr | Dietary 5-Aminolevulinic Acid Alleviates Heat Stress-Induced Renal Injury in Laying Hens by Improving Mitochondrial Quality and Enhancing Antioxidant Activity |
| title_full_unstemmed | Dietary 5-Aminolevulinic Acid Alleviates Heat Stress-Induced Renal Injury in Laying Hens by Improving Mitochondrial Quality and Enhancing Antioxidant Activity |
| title_short | Dietary 5-Aminolevulinic Acid Alleviates Heat Stress-Induced Renal Injury in Laying Hens by Improving Mitochondrial Quality and Enhancing Antioxidant Activity |
| title_sort | dietary 5 aminolevulinic acid alleviates heat stress induced renal injury in laying hens by improving mitochondrial quality and enhancing antioxidant activity |
| topic | cGAS-STING heat stress heme oxygenase-1 laying hens mitochondria renal damage |
| url | https://www.mdpi.com/2076-3921/14/5/556 |
| work_keys_str_mv | AT fumikanantohara dietary5aminolevulinicacidalleviatesheatstressinducedrenalinjuryinlayinghensbyimprovingmitochondrialqualityandenhancingantioxidantactivity AT haruhikoohtsu dietary5aminolevulinicacidalleviatesheatstressinducedrenalinjuryinlayinghensbyimprovingmitochondrialqualityandenhancingantioxidantactivity |