Natural infection of Trypanosoma cruzi in client-owned-dogs from rural Yucatan, Mexico
Abstract Dogs play a crucial role in the transmission cycle of Trypanosoma cruzi and their presence in domicile increases the risk of infection in humans. In Yucatán, Mexico previous studies have reported T. cruzi infection in dogs from both rural and urban areas, which we expanded here, to better u...
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Nature Portfolio
2025-03-01
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| Online Access: | https://doi.org/10.1038/s41598-025-92176-5 |
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| author | Jorge Andrés Calderón-Quintal Maryrose José Escalante-Talavera Christian Florian Teh-Poot María Noe Carrera-Campellone Pedro Pablo Martinez-Vega Victor Manuel Dzul-Huchim Landy Magaly Pech-Pisté Etienne B. Waleckx Liliana Estefanía Villanueva-Lizama Jaime Ortega-Lopez Eric Dumonteil Julio Vladimir Cruz-Chan |
| author_facet | Jorge Andrés Calderón-Quintal Maryrose José Escalante-Talavera Christian Florian Teh-Poot María Noe Carrera-Campellone Pedro Pablo Martinez-Vega Victor Manuel Dzul-Huchim Landy Magaly Pech-Pisté Etienne B. Waleckx Liliana Estefanía Villanueva-Lizama Jaime Ortega-Lopez Eric Dumonteil Julio Vladimir Cruz-Chan |
| author_sort | Jorge Andrés Calderón-Quintal |
| collection | DOAJ |
| description | Abstract Dogs play a crucial role in the transmission cycle of Trypanosoma cruzi and their presence in domicile increases the risk of infection in humans. In Yucatán, Mexico previous studies have reported T. cruzi infection in dogs from both rural and urban areas, which we expanded here, to better understand infection dynamics. A total of 186-dogs were screened for T. cruzi infection by PCR and serology. Parasite burden, genotype, immune response, cardiac alterations, and roaming behavior of the dogs were analyzed. The T. cruzi prevalence was 26.8% (50/186). Genotyping of T. cruzi revealed the predominance of TcI parasites, although most dogs (15/25, 60%) harbored mixed infections with additional DTUs including TcII, TcIV, TcV and TcVI. Antibodies against T. cruzi proteins were detected in > 90% of infected dogs, confirming their immunogenicity in natural infections. Mild ECG abnormalities were present in 40% of infected dogs. A logistic model suggested that the interplay between the host responses to multiple parasite strains could mediate differences in disease severity (P = 0.0002, R2 = 0.65). Finally, parasite diversity and dog roaming behavior support a role of dogs as an important link in T. cruzi transmission cycles among habitats. Together, these data provide a strong rationale to target dogs in integrated Chagas disease control interventions. |
| format | Article |
| id | doaj-art-5a2c1806b9864f5ba0cffad89079242c |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-03-01 |
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| spelling | doaj-art-5a2c1806b9864f5ba0cffad89079242c2025-08-20T02:17:01ZengNature PortfolioScientific Reports2045-23222025-03-0115111110.1038/s41598-025-92176-5Natural infection of Trypanosoma cruzi in client-owned-dogs from rural Yucatan, MexicoJorge Andrés Calderón-Quintal0Maryrose José Escalante-Talavera1Christian Florian Teh-Poot2María Noe Carrera-Campellone3Pedro Pablo Martinez-Vega4Victor Manuel Dzul-Huchim5Landy Magaly Pech-Pisté6Etienne B. Waleckx7Liliana Estefanía Villanueva-Lizama8Jaime Ortega-Lopez9Eric Dumonteil10Julio Vladimir Cruz-Chan11Laboratorio de Parasitología, Centro de Investigaciones Regionales “Dr Hideyo Noguchi”, Universidad Autónoma de YucatánLaboratorio de Parasitología, Centro de Investigaciones Regionales “Dr Hideyo Noguchi”, Universidad Autónoma de YucatánLaboratorio de Parasitología, Centro de Investigaciones Regionales “Dr Hideyo Noguchi”, Universidad Autónoma de YucatánLaboratorio de Parasitología, Centro de Investigaciones Regionales “Dr Hideyo Noguchi”, Universidad Autónoma de YucatánLaboratorio de Parasitología, Centro de Investigaciones Regionales “Dr Hideyo Noguchi”, Universidad Autónoma de YucatánLaboratorio de Parasitología, Centro de Investigaciones Regionales “Dr Hideyo Noguchi”, Universidad Autónoma de YucatánLaboratorio de Parasitología, Centro de Investigaciones Regionales “Dr Hideyo Noguchi”, Universidad Autónoma de YucatánLaboratorio de Parasitología, Centro de Investigaciones Regionales “Dr Hideyo Noguchi”, Universidad Autónoma de YucatánLaboratorio de Parasitología, Centro de Investigaciones Regionales “Dr Hideyo Noguchi”, Universidad Autónoma de YucatánDepartamento de Biotecnología y Bioingeniería, Centro de Investigación y Estudios Avanzados del Instituto Politécnico NacionalDepartment of Tropical Medicine and Infectious Disease, and Vector-Borne and Infectious Disease Research Center, Celia Scott Weatherhead School of Public Health and Tropical Medicine, Tulane UniversityLaboratorio de Parasitología, Centro de Investigaciones Regionales “Dr Hideyo Noguchi”, Universidad Autónoma de YucatánAbstract Dogs play a crucial role in the transmission cycle of Trypanosoma cruzi and their presence in domicile increases the risk of infection in humans. In Yucatán, Mexico previous studies have reported T. cruzi infection in dogs from both rural and urban areas, which we expanded here, to better understand infection dynamics. A total of 186-dogs were screened for T. cruzi infection by PCR and serology. Parasite burden, genotype, immune response, cardiac alterations, and roaming behavior of the dogs were analyzed. The T. cruzi prevalence was 26.8% (50/186). Genotyping of T. cruzi revealed the predominance of TcI parasites, although most dogs (15/25, 60%) harbored mixed infections with additional DTUs including TcII, TcIV, TcV and TcVI. Antibodies against T. cruzi proteins were detected in > 90% of infected dogs, confirming their immunogenicity in natural infections. Mild ECG abnormalities were present in 40% of infected dogs. A logistic model suggested that the interplay between the host responses to multiple parasite strains could mediate differences in disease severity (P = 0.0002, R2 = 0.65). Finally, parasite diversity and dog roaming behavior support a role of dogs as an important link in T. cruzi transmission cycles among habitats. Together, these data provide a strong rationale to target dogs in integrated Chagas disease control interventions.https://doi.org/10.1038/s41598-025-92176-5Trypanosoma CruziDogsPathogenesisChagas disease |
| spellingShingle | Jorge Andrés Calderón-Quintal Maryrose José Escalante-Talavera Christian Florian Teh-Poot María Noe Carrera-Campellone Pedro Pablo Martinez-Vega Victor Manuel Dzul-Huchim Landy Magaly Pech-Pisté Etienne B. Waleckx Liliana Estefanía Villanueva-Lizama Jaime Ortega-Lopez Eric Dumonteil Julio Vladimir Cruz-Chan Natural infection of Trypanosoma cruzi in client-owned-dogs from rural Yucatan, Mexico Scientific Reports Trypanosoma Cruzi Dogs Pathogenesis Chagas disease |
| title | Natural infection of Trypanosoma cruzi in client-owned-dogs from rural Yucatan, Mexico |
| title_full | Natural infection of Trypanosoma cruzi in client-owned-dogs from rural Yucatan, Mexico |
| title_fullStr | Natural infection of Trypanosoma cruzi in client-owned-dogs from rural Yucatan, Mexico |
| title_full_unstemmed | Natural infection of Trypanosoma cruzi in client-owned-dogs from rural Yucatan, Mexico |
| title_short | Natural infection of Trypanosoma cruzi in client-owned-dogs from rural Yucatan, Mexico |
| title_sort | natural infection of trypanosoma cruzi in client owned dogs from rural yucatan mexico |
| topic | Trypanosoma Cruzi Dogs Pathogenesis Chagas disease |
| url | https://doi.org/10.1038/s41598-025-92176-5 |
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