Influenza A hemagglutinin virus-like particles confer protection against influenza B virus infection
Influenza A hemagglutinin (HA), neuraminidase, and/or M2e-containing virus-like particles (VLPs) induce immune responses that contribute to protection against multiple influenza A virus subtypes. In this study, we investigated the protective efficacy of influenza A/H1H3 VLPs against influenza B viru...
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| Format: | Article |
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Taylor & Francis Group
2025-12-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2025.2494702 |
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| author | Jie Mao Ki Back Chu Gi-Deok Eom Keon-Woong Yoon Su In Heo Hae-Ji Kang Sung Soo Kim Fu-Shi Quan |
| author_facet | Jie Mao Ki Back Chu Gi-Deok Eom Keon-Woong Yoon Su In Heo Hae-Ji Kang Sung Soo Kim Fu-Shi Quan |
| author_sort | Jie Mao |
| collection | DOAJ |
| description | Influenza A hemagglutinin (HA), neuraminidase, and/or M2e-containing virus-like particles (VLPs) induce immune responses that contribute to protection against multiple influenza A virus subtypes. In this study, we investigated the protective efficacy of influenza A/H1H3 VLPs against influenza B virus infections (B/Colorado/06/2017 and B/Malaysia/2506/2004, Victoria lineage) in mice. A/H1H3 VLP immunization elicited protection against lethal challenge infections with both B/Colorado and B/Malaysia, significantly reducing lung viral loads and ensuring 100% survival of immunized mice. Sera from A/H1H3VLP-immunized mice recognized inactivated B/Colorado and B/Malaysia virus antigens and enhanced Fc receptor-mediated antibody-dependent cellular cytotoxicity (ADCC) responses. Notably, immune sera reacted with HA, HA1, and HA2 antigens from both B/Colorado and B/Malaysia viruses. A/H1H3VLP immunization also induced lung IgG and IgA antibody responses against HA, HA1, and HA2 of both B viruses, as well as antibody-secreting cell responses (ASC), germinal center B (GC B) responses, and CD4+ T cell responses. Additionally, A/H1H3VLP immunization significantly suppressed pro-inflammatory cytokines responses (IFN-γ, IL-6). These results suggest that A/H1H3 VLP hold promise as a candidate for a universal influenza vaccine capable of providing cross-protection against influenza B virus infection. |
| format | Article |
| id | doaj-art-5a1b7d8c56b74deebdd332ecbce1bd23 |
| institution | OA Journals |
| issn | 2222-1751 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-5a1b7d8c56b74deebdd332ecbce1bd232025-08-20T02:29:46ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512025-12-0114110.1080/22221751.2025.2494702Influenza A hemagglutinin virus-like particles confer protection against influenza B virus infectionJie Mao0Ki Back Chu1Gi-Deok Eom2Keon-Woong Yoon3Su In Heo4Hae-Ji Kang5Sung Soo Kim6Fu-Shi Quan7Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul, Republic of KoreaDepartment of Parasitology, Inje University College of Medicine, Busan, Republic of KoreaDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul, Republic of KoreaDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul, Republic of KoreaDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul, Republic of KoreaDepartment of Microbiology, Dongguk University College of Medicine, Gyeongju, Republic of KoreaMedical Research Center for Bioreaction to Reactive Oxygen Species and Biomedical Science Institute, Core Research Institute (CRI), Kyung Hee University, Seoul, Republic of KoreaMedical Research Center for Bioreaction to Reactive Oxygen Species and Biomedical Science Institute, Core Research Institute (CRI), Kyung Hee University, Seoul, Republic of KoreaInfluenza A hemagglutinin (HA), neuraminidase, and/or M2e-containing virus-like particles (VLPs) induce immune responses that contribute to protection against multiple influenza A virus subtypes. In this study, we investigated the protective efficacy of influenza A/H1H3 VLPs against influenza B virus infections (B/Colorado/06/2017 and B/Malaysia/2506/2004, Victoria lineage) in mice. A/H1H3 VLP immunization elicited protection against lethal challenge infections with both B/Colorado and B/Malaysia, significantly reducing lung viral loads and ensuring 100% survival of immunized mice. Sera from A/H1H3VLP-immunized mice recognized inactivated B/Colorado and B/Malaysia virus antigens and enhanced Fc receptor-mediated antibody-dependent cellular cytotoxicity (ADCC) responses. Notably, immune sera reacted with HA, HA1, and HA2 antigens from both B/Colorado and B/Malaysia viruses. A/H1H3VLP immunization also induced lung IgG and IgA antibody responses against HA, HA1, and HA2 of both B viruses, as well as antibody-secreting cell responses (ASC), germinal center B (GC B) responses, and CD4+ T cell responses. Additionally, A/H1H3VLP immunization significantly suppressed pro-inflammatory cytokines responses (IFN-γ, IL-6). These results suggest that A/H1H3 VLP hold promise as a candidate for a universal influenza vaccine capable of providing cross-protection against influenza B virus infection.https://www.tandfonline.com/doi/10.1080/22221751.2025.2494702Virus-like particlescross-protectionuniversal influenza vaccineantibody responsesviral burden regulation |
| spellingShingle | Jie Mao Ki Back Chu Gi-Deok Eom Keon-Woong Yoon Su In Heo Hae-Ji Kang Sung Soo Kim Fu-Shi Quan Influenza A hemagglutinin virus-like particles confer protection against influenza B virus infection Emerging Microbes and Infections Virus-like particles cross-protection universal influenza vaccine antibody responses viral burden regulation |
| title | Influenza A hemagglutinin virus-like particles confer protection against influenza B virus infection |
| title_full | Influenza A hemagglutinin virus-like particles confer protection against influenza B virus infection |
| title_fullStr | Influenza A hemagglutinin virus-like particles confer protection against influenza B virus infection |
| title_full_unstemmed | Influenza A hemagglutinin virus-like particles confer protection against influenza B virus infection |
| title_short | Influenza A hemagglutinin virus-like particles confer protection against influenza B virus infection |
| title_sort | influenza a hemagglutinin virus like particles confer protection against influenza b virus infection |
| topic | Virus-like particles cross-protection universal influenza vaccine antibody responses viral burden regulation |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2025.2494702 |
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