Physiological and transcriptome analysis of changes in endogenous hormone contents and related synthesis and signaling genes during the heat stress in garlic (Allium sativum L.)
Abstract High-temperature stress severely limits the growth, development, yield, and quality of garlic (Allium sativum L.), but the role of hormone signaling in its heat stress response remains unclear. This study examined changes in seven plant hormones and the expression of related genes in garlic...
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| Format: | Article |
| Language: | English |
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BMC
2025-04-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-025-06346-8 |
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| author | Qing-qing Yang Ji-De Fan Can-yu Liu Yong-qiang Zhao Zhi-sheng Xu Xin-juan Lu Jie Ge Bi-wei Zhang Meng-qian Li Yan Yang Feng Yang |
| author_facet | Qing-qing Yang Ji-De Fan Can-yu Liu Yong-qiang Zhao Zhi-sheng Xu Xin-juan Lu Jie Ge Bi-wei Zhang Meng-qian Li Yan Yang Feng Yang |
| author_sort | Qing-qing Yang |
| collection | DOAJ |
| description | Abstract High-temperature stress severely limits the growth, development, yield, and quality of garlic (Allium sativum L.), but the role of hormone signaling in its heat stress response remains unclear. This study examined changes in seven plant hormones and the expression of related genes in garlic leaves (‘Xusuan No. 6’) under heat stress (38 °C for 0, 2, 4, and 24 h). Growth-promoting hormones, auxin and gibberellic acid, significantly decreased within 2 h of heat stress, while stress-response hormones, including abscisic acid, jasmonic acid, salicylic acid, and ethylene, increased. KEGG pathway analysis revealed significant changes in genes related to hormone biosynthesis and signal transduction, such as NCED and PYR/PYL in the ABA pathway, LOX and OPR in JA biosynthesis, AUX and ARF in IAA signaling, and ERT and ERF in ethylene signaling. A protein–protein interaction network identified 15 hub genes potentially coordinating hormone regulation under heat stress. These findings provide a basis for functional validation of key hormone-related genes in the garlic heat-stress response and suggest potential genetic targets for the development of heat-tolerant garlic varieties. |
| format | Article |
| id | doaj-art-5a15b4554ca349fcbbcb596f151b9c62 |
| institution | OA Journals |
| issn | 1471-2229 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj-art-5a15b4554ca349fcbbcb596f151b9c622025-08-20T02:17:13ZengBMCBMC Plant Biology1471-22292025-04-0125112010.1186/s12870-025-06346-8Physiological and transcriptome analysis of changes in endogenous hormone contents and related synthesis and signaling genes during the heat stress in garlic (Allium sativum L.)Qing-qing Yang0Ji-De Fan1Can-yu Liu2Yong-qiang Zhao3Zhi-sheng Xu4Xin-juan Lu5Jie Ge6Bi-wei Zhang7Meng-qian Li8Yan Yang9Feng Yang10Key Laboratory of Biology and Genetic Breeding of Sweetpotato, Ministry of Agriculture and Rural Affairs, Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai DistrictKey Laboratory of Biology and Genetic Breeding of Sweetpotato, Ministry of Agriculture and Rural Affairs, Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai DistrictKey Laboratory of Biology and Genetic Breeding of Sweetpotato, Ministry of Agriculture and Rural Affairs, Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai DistrictKey Laboratory of Biology and Genetic Breeding of Sweetpotato, Ministry of Agriculture and Rural Affairs, Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai DistrictState Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural UniversityKey Laboratory of Biology and Genetic Breeding of Sweetpotato, Ministry of Agriculture and Rural Affairs, Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai DistrictKey Laboratory of Biology and Genetic Breeding of Sweetpotato, Ministry of Agriculture and Rural Affairs, Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai DistrictKey Laboratory of Biology and Genetic Breeding of Sweetpotato, Ministry of Agriculture and Rural Affairs, Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai DistrictKey Laboratory of Biology and Genetic Breeding of Sweetpotato, Ministry of Agriculture and Rural Affairs, Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai DistrictKey Laboratory of Biology and Genetic Breeding of Sweetpotato, Ministry of Agriculture and Rural Affairs, Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai DistrictKey Laboratory of Biology and Genetic Breeding of Sweetpotato, Ministry of Agriculture and Rural Affairs, Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai DistrictAbstract High-temperature stress severely limits the growth, development, yield, and quality of garlic (Allium sativum L.), but the role of hormone signaling in its heat stress response remains unclear. This study examined changes in seven plant hormones and the expression of related genes in garlic leaves (‘Xusuan No. 6’) under heat stress (38 °C for 0, 2, 4, and 24 h). Growth-promoting hormones, auxin and gibberellic acid, significantly decreased within 2 h of heat stress, while stress-response hormones, including abscisic acid, jasmonic acid, salicylic acid, and ethylene, increased. KEGG pathway analysis revealed significant changes in genes related to hormone biosynthesis and signal transduction, such as NCED and PYR/PYL in the ABA pathway, LOX and OPR in JA biosynthesis, AUX and ARF in IAA signaling, and ERT and ERF in ethylene signaling. A protein–protein interaction network identified 15 hub genes potentially coordinating hormone regulation under heat stress. These findings provide a basis for functional validation of key hormone-related genes in the garlic heat-stress response and suggest potential genetic targets for the development of heat-tolerant garlic varieties.https://doi.org/10.1186/s12870-025-06346-8Garlic (Allium sativum L.)HormoneRNA-seqGene expression analysisHigh-temperature |
| spellingShingle | Qing-qing Yang Ji-De Fan Can-yu Liu Yong-qiang Zhao Zhi-sheng Xu Xin-juan Lu Jie Ge Bi-wei Zhang Meng-qian Li Yan Yang Feng Yang Physiological and transcriptome analysis of changes in endogenous hormone contents and related synthesis and signaling genes during the heat stress in garlic (Allium sativum L.) BMC Plant Biology Garlic (Allium sativum L.) Hormone RNA-seq Gene expression analysis High-temperature |
| title | Physiological and transcriptome analysis of changes in endogenous hormone contents and related synthesis and signaling genes during the heat stress in garlic (Allium sativum L.) |
| title_full | Physiological and transcriptome analysis of changes in endogenous hormone contents and related synthesis and signaling genes during the heat stress in garlic (Allium sativum L.) |
| title_fullStr | Physiological and transcriptome analysis of changes in endogenous hormone contents and related synthesis and signaling genes during the heat stress in garlic (Allium sativum L.) |
| title_full_unstemmed | Physiological and transcriptome analysis of changes in endogenous hormone contents and related synthesis and signaling genes during the heat stress in garlic (Allium sativum L.) |
| title_short | Physiological and transcriptome analysis of changes in endogenous hormone contents and related synthesis and signaling genes during the heat stress in garlic (Allium sativum L.) |
| title_sort | physiological and transcriptome analysis of changes in endogenous hormone contents and related synthesis and signaling genes during the heat stress in garlic allium sativum l |
| topic | Garlic (Allium sativum L.) Hormone RNA-seq Gene expression analysis High-temperature |
| url | https://doi.org/10.1186/s12870-025-06346-8 |
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