Transcriptome‑wide excavation and expression pattern analysis of the NAC transcription factors in methyl jasmonate- and sodium chloride-induced Glycyrrhiza uralensis
Abstract The NAC family is among the most extensive sets of plant-exclusive transcription factors (TFs), which are crucial for various plant development and stress response processes. Although a growing number of studies have been carried out on the NAC family in different species, it has not been c...
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Nature Portfolio
2025-02-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-82151-x |
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| author | Yuan Li Yue Shi Zidong Zhu Xiao Chen Kuangwei Cao Jiatong Li Chunsheng Liu Da Li |
| author_facet | Yuan Li Yue Shi Zidong Zhu Xiao Chen Kuangwei Cao Jiatong Li Chunsheng Liu Da Li |
| author_sort | Yuan Li |
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| description | Abstract The NAC family is among the most extensive sets of plant-exclusive transcription factors (TFs), which are crucial for various plant development and stress response processes. Although a growing number of studies have been carried out on the NAC family in different species, it has not been characterized in Glycyrrhiza uralensis. To thoroughly understand the effects of methyl jasmonate (MeJA) and sodium chloride (NaCl) inductions on NAC TFs and investigate the underlying regulatory mechanism of NAC TFs in response to MeJA and NaCl on the biosynthesis of metabolites, we used transcriptome sequencing combined with qRT‒PCR to explore differential gene expression. Comparative transcriptomic profiling by RNA sequencing (RNA-seq) revealed differentially expressed NAC TFs between MeJA/CK (Mock Control) and NaCl/CK. KEGG pathway analysis revealed that NAC TFs involved in starch and sucrose, carbohydrate, lipid, and amino acid metabolism, as well as terpenoid, polyketide, and flavonoid pathways, can regulate the MeJA- and NaCl-induced responses of G. uralensis. This research lays the groundwork for a thorough comprehension of the regulatory mechanism of NAC TFs in response to MeJA and NaCl induction and their involvement in the accumulation of secondary metabolites, which can provide a scientific basis for the cultivation of high-quality varieties of G. uralensis. |
| format | Article |
| id | doaj-art-5a0e8e414e244ea79aaed5739b79f57c |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-5a0e8e414e244ea79aaed5739b79f57c2025-08-20T02:16:40ZengNature PortfolioScientific Reports2045-23222025-02-0115111210.1038/s41598-024-82151-xTranscriptome‑wide excavation and expression pattern analysis of the NAC transcription factors in methyl jasmonate- and sodium chloride-induced Glycyrrhiza uralensisYuan Li0Yue Shi1Zidong Zhu2Xiao Chen3Kuangwei Cao4Jiatong Li5Chunsheng Liu6Da Li7School of Biology and Medicine, Beijing City UniversitySchool of Biology and Medicine, Beijing City UniversitySchool of Biology and Medicine, Beijing City UniversitySchool of Biology and Medicine, Beijing City UniversitySchool of Biology and Medicine, Beijing City UniversitySchool of Biology and Medicine, Beijing City UniversitySchool of Chinese Materia Medica, Beijing University of Chinese MedicineSchool of Biology and Medicine, Beijing City UniversityAbstract The NAC family is among the most extensive sets of plant-exclusive transcription factors (TFs), which are crucial for various plant development and stress response processes. Although a growing number of studies have been carried out on the NAC family in different species, it has not been characterized in Glycyrrhiza uralensis. To thoroughly understand the effects of methyl jasmonate (MeJA) and sodium chloride (NaCl) inductions on NAC TFs and investigate the underlying regulatory mechanism of NAC TFs in response to MeJA and NaCl on the biosynthesis of metabolites, we used transcriptome sequencing combined with qRT‒PCR to explore differential gene expression. Comparative transcriptomic profiling by RNA sequencing (RNA-seq) revealed differentially expressed NAC TFs between MeJA/CK (Mock Control) and NaCl/CK. KEGG pathway analysis revealed that NAC TFs involved in starch and sucrose, carbohydrate, lipid, and amino acid metabolism, as well as terpenoid, polyketide, and flavonoid pathways, can regulate the MeJA- and NaCl-induced responses of G. uralensis. This research lays the groundwork for a thorough comprehension of the regulatory mechanism of NAC TFs in response to MeJA and NaCl induction and their involvement in the accumulation of secondary metabolites, which can provide a scientific basis for the cultivation of high-quality varieties of G. uralensis.https://doi.org/10.1038/s41598-024-82151-xGlycyrrhiza uralensisNAC transcription factorMeJA inductionNaCl inductionTranscriptome analysis |
| spellingShingle | Yuan Li Yue Shi Zidong Zhu Xiao Chen Kuangwei Cao Jiatong Li Chunsheng Liu Da Li Transcriptome‑wide excavation and expression pattern analysis of the NAC transcription factors in methyl jasmonate- and sodium chloride-induced Glycyrrhiza uralensis Scientific Reports Glycyrrhiza uralensis NAC transcription factor MeJA induction NaCl induction Transcriptome analysis |
| title | Transcriptome‑wide excavation and expression pattern analysis of the NAC transcription factors in methyl jasmonate- and sodium chloride-induced Glycyrrhiza uralensis |
| title_full | Transcriptome‑wide excavation and expression pattern analysis of the NAC transcription factors in methyl jasmonate- and sodium chloride-induced Glycyrrhiza uralensis |
| title_fullStr | Transcriptome‑wide excavation and expression pattern analysis of the NAC transcription factors in methyl jasmonate- and sodium chloride-induced Glycyrrhiza uralensis |
| title_full_unstemmed | Transcriptome‑wide excavation and expression pattern analysis of the NAC transcription factors in methyl jasmonate- and sodium chloride-induced Glycyrrhiza uralensis |
| title_short | Transcriptome‑wide excavation and expression pattern analysis of the NAC transcription factors in methyl jasmonate- and sodium chloride-induced Glycyrrhiza uralensis |
| title_sort | transcriptome wide excavation and expression pattern analysis of the nac transcription factors in methyl jasmonate and sodium chloride induced glycyrrhiza uralensis |
| topic | Glycyrrhiza uralensis NAC transcription factor MeJA induction NaCl induction Transcriptome analysis |
| url | https://doi.org/10.1038/s41598-024-82151-x |
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