Stimulatory effect of medium components on phytase production by Aspergillus niger and biotechnological application as a poultry feed additive

The present study deals with the production of phytase from Aspergillus niger using solid-state fermentation (SSF). Appropriate concentration of different components of the growth medium played an important role for the higher production of phytase. Maximum enzyme activity (265 IU/g) was obtained,...

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Main Authors: Shahzad Mahmood, Memuna G. Shahid, Muhammad Nadeem, Muhammad S. A. Ahmad, Muhammad Irfan
Format: Article
Language:English
Published: Elsevier 2022-07-01
Series:Kuwait Journal of Science
Online Access:https://journalskuwait.org/kjs/index.php/KJS/article/view/17947
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author Shahzad Mahmood
Memuna G. Shahid
Muhammad Nadeem
Muhammad S. A. Ahmad
Muhammad Irfan
author_facet Shahzad Mahmood
Memuna G. Shahid
Muhammad Nadeem
Muhammad S. A. Ahmad
Muhammad Irfan
author_sort Shahzad Mahmood
collection DOAJ
description The present study deals with the production of phytase from Aspergillus niger using solid-state fermentation (SSF). Appropriate concentration of different components of the growth medium played an important role for the higher production of phytase. Maximum enzyme activity (265 IU/g) was obtained, when the fungus was grown in the fermentation medium containing, (% w/w) glucose 1, NH4NO3 0.5, MgSO4.7H2O, KCl  and FeSO4.7H2O 0.1 each, after 5 days of incubation time using pH 6 at 35 °C incubation temperature. Phytase produced during present study was also supplemented as poultry feed additive and given to broiler chickens for 5 weeks. The results exhibited that there was increase in body weight gain (BWG) of chicks for experimental group T3 (3240 g) in comparison to control group (2090 g). The current study indicated that phytase can effectively be supplemented in the broilers diet for better growth performance, recommending the use of phytase as useful additive in poultry feed formulation.
format Article
id doaj-art-5a0e74e3f39d43e08b32fa33a4e67f7d
institution Kabale University
issn 2307-4108
2307-4116
language English
publishDate 2022-07-01
publisher Elsevier
record_format Article
series Kuwait Journal of Science
spelling doaj-art-5a0e74e3f39d43e08b32fa33a4e67f7d2025-08-20T03:26:44ZengElsevierKuwait Journal of Science2307-41082307-41162022-07-01501A10.48129/kjs.17947Stimulatory effect of medium components on phytase production by Aspergillus niger and biotechnological application as a poultry feed additiveShahzad Mahmood0Memuna G. Shahid1Muhammad Nadeem2Muhammad S. A. Ahmad3Muhammad Irfan4Dept. of Botany, Government College University, Katchery Road, Lahore-54000, PakistanDept. of Botany, Government College University, Katchery Road, Lahore-54000, PakistanFood and Biotechnology Research Centre (FBRC), Pakistan Council of Scientific and Industrial Research (PCSIR) Laboratories Complex, Ferozepur Road, Lahore-54600, PakistanDept. of Botany, University of Agriculture, University Road Faisalabad-38000, PakistanDept. of Biotechnology, University of Sargodha, Sargodha-40100, Pakistan The present study deals with the production of phytase from Aspergillus niger using solid-state fermentation (SSF). Appropriate concentration of different components of the growth medium played an important role for the higher production of phytase. Maximum enzyme activity (265 IU/g) was obtained, when the fungus was grown in the fermentation medium containing, (% w/w) glucose 1, NH4NO3 0.5, MgSO4.7H2O, KCl  and FeSO4.7H2O 0.1 each, after 5 days of incubation time using pH 6 at 35 °C incubation temperature. Phytase produced during present study was also supplemented as poultry feed additive and given to broiler chickens for 5 weeks. The results exhibited that there was increase in body weight gain (BWG) of chicks for experimental group T3 (3240 g) in comparison to control group (2090 g). The current study indicated that phytase can effectively be supplemented in the broilers diet for better growth performance, recommending the use of phytase as useful additive in poultry feed formulation. https://journalskuwait.org/kjs/index.php/KJS/article/view/17947
spellingShingle Shahzad Mahmood
Memuna G. Shahid
Muhammad Nadeem
Muhammad S. A. Ahmad
Muhammad Irfan
Stimulatory effect of medium components on phytase production by Aspergillus niger and biotechnological application as a poultry feed additive
Kuwait Journal of Science
title Stimulatory effect of medium components on phytase production by Aspergillus niger and biotechnological application as a poultry feed additive
title_full Stimulatory effect of medium components on phytase production by Aspergillus niger and biotechnological application as a poultry feed additive
title_fullStr Stimulatory effect of medium components on phytase production by Aspergillus niger and biotechnological application as a poultry feed additive
title_full_unstemmed Stimulatory effect of medium components on phytase production by Aspergillus niger and biotechnological application as a poultry feed additive
title_short Stimulatory effect of medium components on phytase production by Aspergillus niger and biotechnological application as a poultry feed additive
title_sort stimulatory effect of medium components on phytase production by aspergillus niger and biotechnological application as a poultry feed additive
url https://journalskuwait.org/kjs/index.php/KJS/article/view/17947
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AT memunagshahid stimulatoryeffectofmediumcomponentsonphytaseproductionbyaspergillusnigerandbiotechnologicalapplicationasapoultryfeedadditive
AT muhammadnadeem stimulatoryeffectofmediumcomponentsonphytaseproductionbyaspergillusnigerandbiotechnologicalapplicationasapoultryfeedadditive
AT muhammadsaahmad stimulatoryeffectofmediumcomponentsonphytaseproductionbyaspergillusnigerandbiotechnologicalapplicationasapoultryfeedadditive
AT muhammadirfan stimulatoryeffectofmediumcomponentsonphytaseproductionbyaspergillusnigerandbiotechnologicalapplicationasapoultryfeedadditive