Assessment of the Biocontrol Efficacy of Silver Nanoparticles Synthesized by <i>Trichoderma asperellum</i> Against Infected <i>Hordeum vulgare</i> L. Germination
This study investigated the biosynthesis, statistical optimization, characterization, and biocontrol activity of silver nanoparticles (AgNPs) produced by newly isolated <i>Trichoderma</i> sp. The <i>Trichoderma asperellum</i> strain TA-3N was identified based on the ITS gene...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
|
| Series: | Life |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-1729/14/12/1560 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850049627613560832 |
|---|---|
| author | Yasmin M. Heikal Nada S. Shweqa Hala M. Abdelmigid Amal A. Alyamani Hoda M. Soliman Noura El-Ahmady El-Naggar |
| author_facet | Yasmin M. Heikal Nada S. Shweqa Hala M. Abdelmigid Amal A. Alyamani Hoda M. Soliman Noura El-Ahmady El-Naggar |
| author_sort | Yasmin M. Heikal |
| collection | DOAJ |
| description | This study investigated the biosynthesis, statistical optimization, characterization, and biocontrol activity of silver nanoparticles (AgNPs) produced by newly isolated <i>Trichoderma</i> sp. The <i>Trichoderma asperellum</i> strain TA-3N was identified based on the ITS gene sequence, together with its phenotypic characteristics (GenBank accession number: OM321439). The color change from light yellow to brown after the incubation period indicates AgNPs biosynthesis. The UV spectrum revealed a single peak with the maximum absorption at 453 nm, indicating that <i>T. asperellum</i> produces AgNPs effectively. A Rotatable Central Composite Design (RCCD) was used to optimize the biosynthesis of AgNPs using the aqueous mycelial-free filtrate of <i>T. asperellum</i>. The optimal conditions for maximum AgNPs biosynthesis (156.02 µg/mL) were predicted theoretically using the desirability function tool and verified experimentally. The highest biosynthetic produced AgNPs by <i>T. asperellum</i> reached 160.3 µg/mL using AgNO<sub>3</sub> concentration of 2 mM/mL, initial pH level of 6, incubation time of 60 h, and biomass weight of 6 g/100 mL water. SEM and TEM imaging revealed uniform spherical shape particles that varied in size between 8.17 and 17.74 nm. The synthesized AgNPs have a Zeta potential value of −9.51 mV. FTIR analysis provided insights into the surface composition of AgNPs, identifying various functional groups such as N–H, -OH, C-H, C=O, and the amide I bond in proteins. Cytotoxicity and genotoxicity assays demonstrated that AgNPs in combination with <i>T. asperellum</i> can mitigate the toxic effects of <i>Fusarium oxysporum</i> on barley. This intervention markedly enhanced cell division rates and decreased chromosomal irregularities. The results indicate that AgNPs synthesized by <i>T. asperellum</i> show the potential as an eco-friendly and efficient method for controlling plant diseases. Further studies are necessary to investigate their possible use in the agricultural sector. |
| format | Article |
| id | doaj-art-5a08313abc794ab9aff9fbd1ecffbef6 |
| institution | DOAJ |
| issn | 2075-1729 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj-art-5a08313abc794ab9aff9fbd1ecffbef62025-08-20T02:53:41ZengMDPI AGLife2075-17292024-11-011412156010.3390/life14121560Assessment of the Biocontrol Efficacy of Silver Nanoparticles Synthesized by <i>Trichoderma asperellum</i> Against Infected <i>Hordeum vulgare</i> L. GerminationYasmin M. Heikal0Nada S. Shweqa1Hala M. Abdelmigid2Amal A. Alyamani3Hoda M. Soliman4Noura El-Ahmady El-Naggar5Botany Department, Faculty of Science, Mansoura University, Mansoura 35516, EgyptBotany Department, Faculty of Science, Mansoura University, Mansoura 35516, EgyptDepartment of Biotechnology, College of Science, Taif University, Taif 21944, Saudi ArabiaDepartment of Biotechnology, College of Science, Taif University, Taif 21944, Saudi ArabiaBotany Department, Faculty of Science, Mansoura University, Mansoura 35516, EgyptDepartment of Bioprocess Development, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El Arab City 21934, EgyptThis study investigated the biosynthesis, statistical optimization, characterization, and biocontrol activity of silver nanoparticles (AgNPs) produced by newly isolated <i>Trichoderma</i> sp. The <i>Trichoderma asperellum</i> strain TA-3N was identified based on the ITS gene sequence, together with its phenotypic characteristics (GenBank accession number: OM321439). The color change from light yellow to brown after the incubation period indicates AgNPs biosynthesis. The UV spectrum revealed a single peak with the maximum absorption at 453 nm, indicating that <i>T. asperellum</i> produces AgNPs effectively. A Rotatable Central Composite Design (RCCD) was used to optimize the biosynthesis of AgNPs using the aqueous mycelial-free filtrate of <i>T. asperellum</i>. The optimal conditions for maximum AgNPs biosynthesis (156.02 µg/mL) were predicted theoretically using the desirability function tool and verified experimentally. The highest biosynthetic produced AgNPs by <i>T. asperellum</i> reached 160.3 µg/mL using AgNO<sub>3</sub> concentration of 2 mM/mL, initial pH level of 6, incubation time of 60 h, and biomass weight of 6 g/100 mL water. SEM and TEM imaging revealed uniform spherical shape particles that varied in size between 8.17 and 17.74 nm. The synthesized AgNPs have a Zeta potential value of −9.51 mV. FTIR analysis provided insights into the surface composition of AgNPs, identifying various functional groups such as N–H, -OH, C-H, C=O, and the amide I bond in proteins. Cytotoxicity and genotoxicity assays demonstrated that AgNPs in combination with <i>T. asperellum</i> can mitigate the toxic effects of <i>Fusarium oxysporum</i> on barley. This intervention markedly enhanced cell division rates and decreased chromosomal irregularities. The results indicate that AgNPs synthesized by <i>T. asperellum</i> show the potential as an eco-friendly and efficient method for controlling plant diseases. Further studies are necessary to investigate their possible use in the agricultural sector.https://www.mdpi.com/2075-1729/14/12/1560silver nanoparticlesbiosynthesis<i>Trichoderma asperellum</i>statistical optimizationcharacterizationcyto-genotoxicity assay |
| spellingShingle | Yasmin M. Heikal Nada S. Shweqa Hala M. Abdelmigid Amal A. Alyamani Hoda M. Soliman Noura El-Ahmady El-Naggar Assessment of the Biocontrol Efficacy of Silver Nanoparticles Synthesized by <i>Trichoderma asperellum</i> Against Infected <i>Hordeum vulgare</i> L. Germination Life silver nanoparticles biosynthesis <i>Trichoderma asperellum</i> statistical optimization characterization cyto-genotoxicity assay |
| title | Assessment of the Biocontrol Efficacy of Silver Nanoparticles Synthesized by <i>Trichoderma asperellum</i> Against Infected <i>Hordeum vulgare</i> L. Germination |
| title_full | Assessment of the Biocontrol Efficacy of Silver Nanoparticles Synthesized by <i>Trichoderma asperellum</i> Against Infected <i>Hordeum vulgare</i> L. Germination |
| title_fullStr | Assessment of the Biocontrol Efficacy of Silver Nanoparticles Synthesized by <i>Trichoderma asperellum</i> Against Infected <i>Hordeum vulgare</i> L. Germination |
| title_full_unstemmed | Assessment of the Biocontrol Efficacy of Silver Nanoparticles Synthesized by <i>Trichoderma asperellum</i> Against Infected <i>Hordeum vulgare</i> L. Germination |
| title_short | Assessment of the Biocontrol Efficacy of Silver Nanoparticles Synthesized by <i>Trichoderma asperellum</i> Against Infected <i>Hordeum vulgare</i> L. Germination |
| title_sort | assessment of the biocontrol efficacy of silver nanoparticles synthesized by i trichoderma asperellum i against infected i hordeum vulgare i l germination |
| topic | silver nanoparticles biosynthesis <i>Trichoderma asperellum</i> statistical optimization characterization cyto-genotoxicity assay |
| url | https://www.mdpi.com/2075-1729/14/12/1560 |
| work_keys_str_mv | AT yasminmheikal assessmentofthebiocontrolefficacyofsilvernanoparticlessynthesizedbyitrichodermaasperellumiagainstinfectedihordeumvulgareilgermination AT nadasshweqa assessmentofthebiocontrolefficacyofsilvernanoparticlessynthesizedbyitrichodermaasperellumiagainstinfectedihordeumvulgareilgermination AT halamabdelmigid assessmentofthebiocontrolefficacyofsilvernanoparticlessynthesizedbyitrichodermaasperellumiagainstinfectedihordeumvulgareilgermination AT amalaalyamani assessmentofthebiocontrolefficacyofsilvernanoparticlessynthesizedbyitrichodermaasperellumiagainstinfectedihordeumvulgareilgermination AT hodamsoliman assessmentofthebiocontrolefficacyofsilvernanoparticlessynthesizedbyitrichodermaasperellumiagainstinfectedihordeumvulgareilgermination AT nouraelahmadyelnaggar assessmentofthebiocontrolefficacyofsilvernanoparticlessynthesizedbyitrichodermaasperellumiagainstinfectedihordeumvulgareilgermination |