Impaired Surface Expression of HLA-DR, TLR2, TLR4, and TLR9 in Ex Vivo-In Vitro Stimulated Monocytes from Severely Injured Trauma Patients
Objective. Trauma patients (TP) frequently develop an imbalanced immune response that often causes infectious postinjury complications. Monocytes show a diminished capability of both producing proinflammatory cytokines and antigen presentation after trauma. TLR2, TLR4, and TLR9 recognize pathogens a...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2017/2608349 |
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| author | David Heftrig Ramona Sturm Elsie Oppermann Kerstin Kontradowitz Katrin Jurida Lukas Schimunek Mathias Woschek Ingo Marzi Borna Relja |
| author_facet | David Heftrig Ramona Sturm Elsie Oppermann Kerstin Kontradowitz Katrin Jurida Lukas Schimunek Mathias Woschek Ingo Marzi Borna Relja |
| author_sort | David Heftrig |
| collection | DOAJ |
| description | Objective. Trauma patients (TP) frequently develop an imbalanced immune response that often causes infectious postinjury complications. Monocytes show a diminished capability of both producing proinflammatory cytokines and antigen presentation after trauma. TLR2, TLR4, and TLR9 recognize pathogens and subsequently activate monocytes. While there are conflictive data about TLR2 and TLR4 expression after trauma, no studies about the expression of TLR2, TLR4, TLR9, and HLA-DR on monocytes from TP after their secondary ex vivo-in vitro “hit” have been reported. Methods/Results. Ex vivo-in vitro lipopolysaccharide- (LPS-) stimulated blood from TP showed diminished interleukin- (IL-) 1β-release in TP for five postinjury days compared to healthy volunteers (HV). The recovery was observed at day 5. In parallel, monocytes from TP showed an impaired capability of TLR2, TLR4, and TLR9 expression after secondary stimulation compared to HV, while the measurement of unstimulated samples showed significant reduction of TLR4 and TLR9 at ED. Furthermore, HLA-DR decreased after trauma and was even more profound by stimulation of monocytes. Ratio of monocytes to leukocytes was significantly increased at days 6 and 7 after trauma compared to HV. Conclusion. Impaired expression of TLRs and HLA-DR in acute inflammatory conditions may be responsible for the well-described monocyte paralysis after severe trauma. |
| format | Article |
| id | doaj-art-5a033a515149485e92c4c874aee1d015 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-5a033a515149485e92c4c874aee1d0152025-08-20T03:34:31ZengWileyMediators of Inflammation0962-93511466-18612017-01-01201710.1155/2017/26083492608349Impaired Surface Expression of HLA-DR, TLR2, TLR4, and TLR9 in Ex Vivo-In Vitro Stimulated Monocytes from Severely Injured Trauma PatientsDavid Heftrig0Ramona Sturm1Elsie Oppermann2Kerstin Kontradowitz3Katrin Jurida4Lukas Schimunek5Mathias Woschek6Ingo Marzi7Borna Relja8Department of Trauma, Hand and Reconstructive Surgery, Goethe University, Frankfurt, GermanyDepartment of Trauma, Hand and Reconstructive Surgery, Goethe University, Frankfurt, GermanyDepartment of General and Visceral Surgery, Goethe University, Frankfurt, GermanyDepartment of Trauma, Hand and Reconstructive Surgery, Goethe University, Frankfurt, GermanyDepartment of Trauma, Hand and Reconstructive Surgery, Goethe University, Frankfurt, GermanyDepartment of Trauma, Hand and Reconstructive Surgery, Goethe University, Frankfurt, GermanyDepartment of Trauma, Hand and Reconstructive Surgery, Goethe University, Frankfurt, GermanyDepartment of Trauma, Hand and Reconstructive Surgery, Goethe University, Frankfurt, GermanyDepartment of Trauma, Hand and Reconstructive Surgery, Goethe University, Frankfurt, GermanyObjective. Trauma patients (TP) frequently develop an imbalanced immune response that often causes infectious postinjury complications. Monocytes show a diminished capability of both producing proinflammatory cytokines and antigen presentation after trauma. TLR2, TLR4, and TLR9 recognize pathogens and subsequently activate monocytes. While there are conflictive data about TLR2 and TLR4 expression after trauma, no studies about the expression of TLR2, TLR4, TLR9, and HLA-DR on monocytes from TP after their secondary ex vivo-in vitro “hit” have been reported. Methods/Results. Ex vivo-in vitro lipopolysaccharide- (LPS-) stimulated blood from TP showed diminished interleukin- (IL-) 1β-release in TP for five postinjury days compared to healthy volunteers (HV). The recovery was observed at day 5. In parallel, monocytes from TP showed an impaired capability of TLR2, TLR4, and TLR9 expression after secondary stimulation compared to HV, while the measurement of unstimulated samples showed significant reduction of TLR4 and TLR9 at ED. Furthermore, HLA-DR decreased after trauma and was even more profound by stimulation of monocytes. Ratio of monocytes to leukocytes was significantly increased at days 6 and 7 after trauma compared to HV. Conclusion. Impaired expression of TLRs and HLA-DR in acute inflammatory conditions may be responsible for the well-described monocyte paralysis after severe trauma.http://dx.doi.org/10.1155/2017/2608349 |
| spellingShingle | David Heftrig Ramona Sturm Elsie Oppermann Kerstin Kontradowitz Katrin Jurida Lukas Schimunek Mathias Woschek Ingo Marzi Borna Relja Impaired Surface Expression of HLA-DR, TLR2, TLR4, and TLR9 in Ex Vivo-In Vitro Stimulated Monocytes from Severely Injured Trauma Patients Mediators of Inflammation |
| title | Impaired Surface Expression of HLA-DR, TLR2, TLR4, and TLR9 in Ex Vivo-In Vitro Stimulated Monocytes from Severely Injured Trauma Patients |
| title_full | Impaired Surface Expression of HLA-DR, TLR2, TLR4, and TLR9 in Ex Vivo-In Vitro Stimulated Monocytes from Severely Injured Trauma Patients |
| title_fullStr | Impaired Surface Expression of HLA-DR, TLR2, TLR4, and TLR9 in Ex Vivo-In Vitro Stimulated Monocytes from Severely Injured Trauma Patients |
| title_full_unstemmed | Impaired Surface Expression of HLA-DR, TLR2, TLR4, and TLR9 in Ex Vivo-In Vitro Stimulated Monocytes from Severely Injured Trauma Patients |
| title_short | Impaired Surface Expression of HLA-DR, TLR2, TLR4, and TLR9 in Ex Vivo-In Vitro Stimulated Monocytes from Severely Injured Trauma Patients |
| title_sort | impaired surface expression of hla dr tlr2 tlr4 and tlr9 in ex vivo in vitro stimulated monocytes from severely injured trauma patients |
| url | http://dx.doi.org/10.1155/2017/2608349 |
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