The Slit–Robo signalling pathway in nervous system development: a comparative perspective from vertebrates and invertebrates

During nervous system development, growing axons find their targets with the help of guidance cues. These cues, which can be secreted molecules provided by neighbouring cells or transmembrane proteins mediating cell–cell contacts with the growing axons, act as either chemoattractants or chemorepelle...

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Main Authors: Nicole Sanhueza, Evelyn C. Avilés, Carlos Oliva
Format: Article
Language:English
Published: The Royal Society 2025-07-01
Series:Open Biology
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Online Access:https://royalsocietypublishing.org/doi/10.1098/rsob.250026
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author Nicole Sanhueza
Evelyn C. Avilés
Carlos Oliva
author_facet Nicole Sanhueza
Evelyn C. Avilés
Carlos Oliva
author_sort Nicole Sanhueza
collection DOAJ
description During nervous system development, growing axons find their targets with the help of guidance cues. These cues, which can be secreted molecules provided by neighbouring cells or transmembrane proteins mediating cell–cell contacts with the growing axons, act as either chemoattractants or chemorepellents. Over the last decades, several axon guidance molecules have been identified. One of the classical guidance cues is the Slit protein. Slit is a secreted protein, initially identified in a genetic screen in the fruit fly Drosophila melanogaster but later shown to be present in other organisms including vertebrates. Slit was originally classified as a repellent guidance cue, but nowadays it is recognized as a promoter of axonal growth in some contexts. Slit action is mediated mainly by the Roundabout (Robo) family of single pass transmembrane proteins, although it has been shown more recently that other proteins can also function as Slit receptors. In this review, we describe the main aspects of Slit–Robo signalling during development of the nervous system. We start with a historical view of the discovery of these proteins, followed by a description of their main molecular characteristics. We then explore specific examples that describe the functions and signal transduction mechanisms of this signalling pathway.
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spelling doaj-art-59fde7ff8f6a421e8b5044d4ae9a47952025-08-20T03:34:45ZengThe Royal SocietyOpen Biology2046-24412025-07-0115710.1098/rsob.250026The Slit–Robo signalling pathway in nervous system development: a comparative perspective from vertebrates and invertebratesNicole Sanhueza0Evelyn C. Avilés1Carlos Oliva2Faculty of Biological Sciences, Pontificia Universidad Católica de Chile, Santiago, Metropolitan Region 8331150, ChileFaculty of Biological Sciences, Pontificia Universidad Católica de Chile, Santiago, Metropolitan Region 8331150, ChileFaculty of Biological Sciences, Pontificia Universidad Católica de Chile, Santiago, Metropolitan Region 8331150, ChileDuring nervous system development, growing axons find their targets with the help of guidance cues. These cues, which can be secreted molecules provided by neighbouring cells or transmembrane proteins mediating cell–cell contacts with the growing axons, act as either chemoattractants or chemorepellents. Over the last decades, several axon guidance molecules have been identified. One of the classical guidance cues is the Slit protein. Slit is a secreted protein, initially identified in a genetic screen in the fruit fly Drosophila melanogaster but later shown to be present in other organisms including vertebrates. Slit was originally classified as a repellent guidance cue, but nowadays it is recognized as a promoter of axonal growth in some contexts. Slit action is mediated mainly by the Roundabout (Robo) family of single pass transmembrane proteins, although it has been shown more recently that other proteins can also function as Slit receptors. In this review, we describe the main aspects of Slit–Robo signalling during development of the nervous system. We start with a historical view of the discovery of these proteins, followed by a description of their main molecular characteristics. We then explore specific examples that describe the functions and signal transduction mechanisms of this signalling pathway.https://royalsocietypublishing.org/doi/10.1098/rsob.250026Slit–Robo signallingaxonal guidanceneuronal development
spellingShingle Nicole Sanhueza
Evelyn C. Avilés
Carlos Oliva
The Slit–Robo signalling pathway in nervous system development: a comparative perspective from vertebrates and invertebrates
Open Biology
Slit–Robo signalling
axonal guidance
neuronal development
title The Slit–Robo signalling pathway in nervous system development: a comparative perspective from vertebrates and invertebrates
title_full The Slit–Robo signalling pathway in nervous system development: a comparative perspective from vertebrates and invertebrates
title_fullStr The Slit–Robo signalling pathway in nervous system development: a comparative perspective from vertebrates and invertebrates
title_full_unstemmed The Slit–Robo signalling pathway in nervous system development: a comparative perspective from vertebrates and invertebrates
title_short The Slit–Robo signalling pathway in nervous system development: a comparative perspective from vertebrates and invertebrates
title_sort slit robo signalling pathway in nervous system development a comparative perspective from vertebrates and invertebrates
topic Slit–Robo signalling
axonal guidance
neuronal development
url https://royalsocietypublishing.org/doi/10.1098/rsob.250026
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