Discovery of benzochalcone derivative as a potential antigastric cancer agent targeting signal transducer and activator of transcription 3 (STAT3)

Gastric cancer remains a significant health burden worldwide. In continuation of our previous study and development of effective small molecules against gastric cancer, a series of benzochalcone analogues involving heterocyclic molecules were synthesised and biologically evaluated in vitro and in vi...

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Main Authors: Jinyun Dong, Jing Yang, Wenkai Yu, Haobin Li, Maohua Cai, Jing-Li Xu, Han-Dong Xu, Yun-Fu Shi, Xiaoqing Guan, Xiang‑Dong Cheng, Jiang‑Jiang Qin
Format: Article
Language:English
Published: Taylor & Francis Group 2022-12-01
Series:Journal of Enzyme Inhibition and Medicinal Chemistry
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Online Access:https://www.tandfonline.com/doi/10.1080/14756366.2022.2100366
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author Jinyun Dong
Jing Yang
Wenkai Yu
Haobin Li
Maohua Cai
Jing-Li Xu
Han-Dong Xu
Yun-Fu Shi
Xiaoqing Guan
Xiang‑Dong Cheng
Jiang‑Jiang Qin
author_facet Jinyun Dong
Jing Yang
Wenkai Yu
Haobin Li
Maohua Cai
Jing-Li Xu
Han-Dong Xu
Yun-Fu Shi
Xiaoqing Guan
Xiang‑Dong Cheng
Jiang‑Jiang Qin
author_sort Jinyun Dong
collection DOAJ
description Gastric cancer remains a significant health burden worldwide. In continuation of our previous study and development of effective small molecules against gastric cancer, a series of benzochalcone analogues involving heterocyclic molecules were synthesised and biologically evaluated in vitro and in vivo. Among them, the quinolin-6-yl substituted derivative KL-6 inhibited the growth of gastric cancer cells (HGC27, MKN28, AZ521, AGS, and MKN1) with a submicromolar to micromolar range of IC50, being the most potent one in this series. Additionally, KL-6 significantly inhibited the colony formation, migration and invasion, and effectively induced apoptosis of MKN1 cells in a concentration-dependent manner. The mechanistic study revealed that KL-6 could concentration-dependently suppress STAT3 phosphorylation, which may partly contribute to its anticancer activity. Furthermore, in vivo antitumour study on the MKN1 orthotopic tumour model showed that KL-6 effectively inhibited tumour growth (TGI of 78%) and metastasis without obvious toxicity. Collectively, compound KL-6 may support the further development of candidates for gastric cancer treatment.
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issn 1475-6366
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publishDate 2022-12-01
publisher Taylor & Francis Group
record_format Article
series Journal of Enzyme Inhibition and Medicinal Chemistry
spelling doaj-art-59f779a14cb74fd68b924c8ac81f8a4e2025-08-20T03:31:27ZengTaylor & Francis GroupJournal of Enzyme Inhibition and Medicinal Chemistry1475-63661475-63742022-12-013712004201610.1080/14756366.2022.2100366Discovery of benzochalcone derivative as a potential antigastric cancer agent targeting signal transducer and activator of transcription 3 (STAT3)Jinyun Dong0Jing Yang1Wenkai Yu2Haobin Li3Maohua Cai4Jing-Li Xu5Han-Dong Xu6Yun-Fu Shi7Xiaoqing Guan8Xiang‑Dong Cheng9Jiang‑Jiang Qin10The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, ChinaSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, ChinaSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, ChinaSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, ChinaSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, ChinaThe First Clinical Medical College of Zhejiang, Chinese Medical University, Hangzhou, ChinaThe First Clinical Medical College of Zhejiang, Chinese Medical University, Hangzhou, ChinaThe First Clinical Medical College of Zhejiang, Chinese Medical University, Hangzhou, ChinaThe Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, ChinaThe Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, ChinaThe Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, ChinaGastric cancer remains a significant health burden worldwide. In continuation of our previous study and development of effective small molecules against gastric cancer, a series of benzochalcone analogues involving heterocyclic molecules were synthesised and biologically evaluated in vitro and in vivo. Among them, the quinolin-6-yl substituted derivative KL-6 inhibited the growth of gastric cancer cells (HGC27, MKN28, AZ521, AGS, and MKN1) with a submicromolar to micromolar range of IC50, being the most potent one in this series. Additionally, KL-6 significantly inhibited the colony formation, migration and invasion, and effectively induced apoptosis of MKN1 cells in a concentration-dependent manner. The mechanistic study revealed that KL-6 could concentration-dependently suppress STAT3 phosphorylation, which may partly contribute to its anticancer activity. Furthermore, in vivo antitumour study on the MKN1 orthotopic tumour model showed that KL-6 effectively inhibited tumour growth (TGI of 78%) and metastasis without obvious toxicity. Collectively, compound KL-6 may support the further development of candidates for gastric cancer treatment.https://www.tandfonline.com/doi/10.1080/14756366.2022.2100366Benzochalcone analoguesSTAT3gastric cancer
spellingShingle Jinyun Dong
Jing Yang
Wenkai Yu
Haobin Li
Maohua Cai
Jing-Li Xu
Han-Dong Xu
Yun-Fu Shi
Xiaoqing Guan
Xiang‑Dong Cheng
Jiang‑Jiang Qin
Discovery of benzochalcone derivative as a potential antigastric cancer agent targeting signal transducer and activator of transcription 3 (STAT3)
Journal of Enzyme Inhibition and Medicinal Chemistry
Benzochalcone analogues
STAT3
gastric cancer
title Discovery of benzochalcone derivative as a potential antigastric cancer agent targeting signal transducer and activator of transcription 3 (STAT3)
title_full Discovery of benzochalcone derivative as a potential antigastric cancer agent targeting signal transducer and activator of transcription 3 (STAT3)
title_fullStr Discovery of benzochalcone derivative as a potential antigastric cancer agent targeting signal transducer and activator of transcription 3 (STAT3)
title_full_unstemmed Discovery of benzochalcone derivative as a potential antigastric cancer agent targeting signal transducer and activator of transcription 3 (STAT3)
title_short Discovery of benzochalcone derivative as a potential antigastric cancer agent targeting signal transducer and activator of transcription 3 (STAT3)
title_sort discovery of benzochalcone derivative as a potential antigastric cancer agent targeting signal transducer and activator of transcription 3 stat3
topic Benzochalcone analogues
STAT3
gastric cancer
url https://www.tandfonline.com/doi/10.1080/14756366.2022.2100366
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