Exploring the Effects of Artemisia absinthium L. Essential Oil on Pseudopregnancy Model in Rats

ABSTRACT Pseudopregnancy is a luteal phase syndrome characterized by symptoms of late pregnancy and/or early postpartum periods. This phenomenon is observed in various species, including rats, rabbits and dogs, and arises due to hormonal alterations. Due to the significant side effects of current tr...

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Main Authors: Mürşide Ayşe Demirel, İpek Süntar, Ali Osman Çeribaşı, Kevser Taban, Gökhan Zengin
Format: Article
Language:English
Published: Wiley 2025-09-01
Series:Veterinary Medicine and Science
Subjects:
Online Access:https://doi.org/10.1002/vms3.70582
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author Mürşide Ayşe Demirel
İpek Süntar
Ali Osman Çeribaşı
Kevser Taban
Gökhan Zengin
author_facet Mürşide Ayşe Demirel
İpek Süntar
Ali Osman Çeribaşı
Kevser Taban
Gökhan Zengin
author_sort Mürşide Ayşe Demirel
collection DOAJ
description ABSTRACT Pseudopregnancy is a luteal phase syndrome characterized by symptoms of late pregnancy and/or early postpartum periods. This phenomenon is observed in various species, including rats, rabbits and dogs, and arises due to hormonal alterations. Due to the significant side effects of current treatment regimens, there is a growing need for the development of alternative therapeutic strategies. In this context, various plants and natural compounds have been investigated for their potential to modulate hormonal balance, offering a promising avenue for the treatment of pseudopregnancy. Artemisia absinthium L., a medicinal plant traditionally utilized as an emmenagogue and abortifacient, is known for its regulatory effect on the dopaminergic system. The study aimed to assess whether A. absinthium treatment could restore oestrous cyclicity in pseudopregnant rats. Pseudopregnancy was induced through the administration of a pregnant mare's serum gonadotropin and human chorionic gonadotropin in female rats. The essential oil of A. absinthium was administered orally at doses of 12.5, 25 and 50 mg/kg once daily for 10 days, with bromocriptine as a reference treatment. The results indicated that the 25 mg/kg dose of A. absinthium essential oil exhibited beneficial effects in the pseudopregnancy model. Gas chromatography analysis revealed that the major components of the essential oil included cis‐chrysanthenyl acetate (17.8%), sabinyl acetate (11.6%), terpinen‐4‐ol (6.2%), caryophyllene oxide (5.5%) and (E)‐nuciferol (5.5%). These findings suggest that A. absinthium essential oil may present a promising therapeutic option for the management of pseudopregnancy.
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spelling doaj-art-59ee289831fa459ea6380c675eeae4db2025-08-22T15:01:52ZengWileyVeterinary Medicine and Science2053-10952025-09-01115n/an/a10.1002/vms3.70582Exploring the Effects of Artemisia absinthium L. Essential Oil on Pseudopregnancy Model in RatsMürşide Ayşe Demirel0İpek Süntar1Ali Osman Çeribaşı2Kevser Taban3Gökhan Zengin4Laboratory Animals Breeding and Experimental Researches Center, Department of Basic Pharmaceutical Sciences, Faculty of Pharmacy Gazi University Ankara TurkeyDepartment of Pharmacognosy, Faculty of Pharmacy Gazi University Ankara TurkeyDepartment of Pathology, Faculty of Veterinary Medicine Fırat University Elazig TurkeyDepartment of Pharmacognosy, Faculty of Pharmacy Sivas Cumhuriyet University Sivas TurkeyDepartment of Biology, Science Faculty Selcuk University Konya TurkeyABSTRACT Pseudopregnancy is a luteal phase syndrome characterized by symptoms of late pregnancy and/or early postpartum periods. This phenomenon is observed in various species, including rats, rabbits and dogs, and arises due to hormonal alterations. Due to the significant side effects of current treatment regimens, there is a growing need for the development of alternative therapeutic strategies. In this context, various plants and natural compounds have been investigated for their potential to modulate hormonal balance, offering a promising avenue for the treatment of pseudopregnancy. Artemisia absinthium L., a medicinal plant traditionally utilized as an emmenagogue and abortifacient, is known for its regulatory effect on the dopaminergic system. The study aimed to assess whether A. absinthium treatment could restore oestrous cyclicity in pseudopregnant rats. Pseudopregnancy was induced through the administration of a pregnant mare's serum gonadotropin and human chorionic gonadotropin in female rats. The essential oil of A. absinthium was administered orally at doses of 12.5, 25 and 50 mg/kg once daily for 10 days, with bromocriptine as a reference treatment. The results indicated that the 25 mg/kg dose of A. absinthium essential oil exhibited beneficial effects in the pseudopregnancy model. Gas chromatography analysis revealed that the major components of the essential oil included cis‐chrysanthenyl acetate (17.8%), sabinyl acetate (11.6%), terpinen‐4‐ol (6.2%), caryophyllene oxide (5.5%) and (E)‐nuciferol (5.5%). These findings suggest that A. absinthium essential oil may present a promising therapeutic option for the management of pseudopregnancy.https://doi.org/10.1002/vms3.70582Artemisia absinthiumAsteraceaeessential oilin vivopseudopregnancy
spellingShingle Mürşide Ayşe Demirel
İpek Süntar
Ali Osman Çeribaşı
Kevser Taban
Gökhan Zengin
Exploring the Effects of Artemisia absinthium L. Essential Oil on Pseudopregnancy Model in Rats
Veterinary Medicine and Science
Artemisia absinthium
Asteraceae
essential oil
in vivo
pseudopregnancy
title Exploring the Effects of Artemisia absinthium L. Essential Oil on Pseudopregnancy Model in Rats
title_full Exploring the Effects of Artemisia absinthium L. Essential Oil on Pseudopregnancy Model in Rats
title_fullStr Exploring the Effects of Artemisia absinthium L. Essential Oil on Pseudopregnancy Model in Rats
title_full_unstemmed Exploring the Effects of Artemisia absinthium L. Essential Oil on Pseudopregnancy Model in Rats
title_short Exploring the Effects of Artemisia absinthium L. Essential Oil on Pseudopregnancy Model in Rats
title_sort exploring the effects of artemisia absinthium l essential oil on pseudopregnancy model in rats
topic Artemisia absinthium
Asteraceae
essential oil
in vivo
pseudopregnancy
url https://doi.org/10.1002/vms3.70582
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