Human adenovirus species B knob proteins as immunogens for inducing cross-neutralizing antibody responses
ABSTRACT The re-emerging human adenovirus (HAdV) types 3, 7, 14, and 55 of species B have caused severe or even fatal acute respiratory disease. Therefore, the development of multivalent vaccines against HAdV types 3, 7, 14, and 55 remains an important goal. In our previous study, we identified a cr...
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American Society for Microbiology
2025-01-01
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| Online Access: | https://journals.asm.org/doi/10.1128/msphere.00644-24 |
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| author | Zhenwei Liu Yuting Xian Jixian Lan Zhichao Zhou Xiao Li Rong Zhou Dehui Chen Xingui Tian |
| author_facet | Zhenwei Liu Yuting Xian Jixian Lan Zhichao Zhou Xiao Li Rong Zhou Dehui Chen Xingui Tian |
| author_sort | Zhenwei Liu |
| collection | DOAJ |
| description | ABSTRACT The re-emerging human adenovirus (HAdV) types 3, 7, 14, and 55 of species B have caused severe or even fatal acute respiratory disease. Therefore, the development of multivalent vaccines against HAdV types 3, 7, 14, and 55 remains an important goal. In our previous study, we identified a cross-neutralizing epitope that induced broadly reactive monoclonal neutralizing antibodies against the knob proteins of HAdV types 7, 11, 14, and 55. To study the immunogenicity of HAdV species B knob proteins, we evaluated humoral immune responses to the knob proteins in vivo and in vitro. We found that the knob proteins elicited robust binding and neutralizing antibody responses after three immunizations of mice. In addition, mouse antisera raised against the knob proteins exhibited cross-neutralizing activity against original species B members. Furthermore, immunization with a mix of HAdV-3, 7, and 55 knob proteins protected Chinese tree shrews against an experimental HAdV challenge. Our results provide insight into the immunogenicity of HAdV species B knob proteins.IMPORTANCEHuman adenovirus (HAdV) species B are common pathogens causing severe pneumonia in children, and there is currently no vaccine available. Because there are many HAdV species B types, developing broad-spectrum vaccines against HAdV species B is an important research goal. Our study revealed that immunization with recombinant HAdV species B knob proteins effectively elicited cross-neutralizing antibody responses against original species B members with protective immunity. This study provides a novel insight into the immunogenicity of HAdV species B knob proteins. |
| format | Article |
| id | doaj-art-59ee27fa54434231828449a9a49872cc |
| institution | Kabale University |
| issn | 2379-5042 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mSphere |
| spelling | doaj-art-59ee27fa54434231828449a9a49872cc2025-01-28T14:00:56ZengAmerican Society for MicrobiologymSphere2379-50422025-01-0110110.1128/msphere.00644-24Human adenovirus species B knob proteins as immunogens for inducing cross-neutralizing antibody responsesZhenwei Liu0Yuting Xian1Jixian Lan2Zhichao Zhou3Xiao Li4Rong Zhou5Dehui Chen6Xingui Tian7Department of Pediatrics, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, ChinaGuangdong Xinmai Biotechnology Co., Ltd, Guangzhou, ChinaDeep Evolution (Guangzhou) Biotechnology Co., Ltd, Guangzhou, ChinaState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, ChinaState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, ChinaState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, ChinaDepartment of Pediatrics, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, ChinaState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, ChinaABSTRACT The re-emerging human adenovirus (HAdV) types 3, 7, 14, and 55 of species B have caused severe or even fatal acute respiratory disease. Therefore, the development of multivalent vaccines against HAdV types 3, 7, 14, and 55 remains an important goal. In our previous study, we identified a cross-neutralizing epitope that induced broadly reactive monoclonal neutralizing antibodies against the knob proteins of HAdV types 7, 11, 14, and 55. To study the immunogenicity of HAdV species B knob proteins, we evaluated humoral immune responses to the knob proteins in vivo and in vitro. We found that the knob proteins elicited robust binding and neutralizing antibody responses after three immunizations of mice. In addition, mouse antisera raised against the knob proteins exhibited cross-neutralizing activity against original species B members. Furthermore, immunization with a mix of HAdV-3, 7, and 55 knob proteins protected Chinese tree shrews against an experimental HAdV challenge. Our results provide insight into the immunogenicity of HAdV species B knob proteins.IMPORTANCEHuman adenovirus (HAdV) species B are common pathogens causing severe pneumonia in children, and there is currently no vaccine available. Because there are many HAdV species B types, developing broad-spectrum vaccines against HAdV species B is an important research goal. Our study revealed that immunization with recombinant HAdV species B knob proteins effectively elicited cross-neutralizing antibody responses against original species B members with protective immunity. This study provides a novel insight into the immunogenicity of HAdV species B knob proteins.https://journals.asm.org/doi/10.1128/msphere.00644-24human adenovirus species Bknob proteincross-neutralizing antibody response |
| spellingShingle | Zhenwei Liu Yuting Xian Jixian Lan Zhichao Zhou Xiao Li Rong Zhou Dehui Chen Xingui Tian Human adenovirus species B knob proteins as immunogens for inducing cross-neutralizing antibody responses mSphere human adenovirus species B knob protein cross-neutralizing antibody response |
| title | Human adenovirus species B knob proteins as immunogens for inducing cross-neutralizing antibody responses |
| title_full | Human adenovirus species B knob proteins as immunogens for inducing cross-neutralizing antibody responses |
| title_fullStr | Human adenovirus species B knob proteins as immunogens for inducing cross-neutralizing antibody responses |
| title_full_unstemmed | Human adenovirus species B knob proteins as immunogens for inducing cross-neutralizing antibody responses |
| title_short | Human adenovirus species B knob proteins as immunogens for inducing cross-neutralizing antibody responses |
| title_sort | human adenovirus species b knob proteins as immunogens for inducing cross neutralizing antibody responses |
| topic | human adenovirus species B knob protein cross-neutralizing antibody response |
| url | https://journals.asm.org/doi/10.1128/msphere.00644-24 |
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