RETRACTED ARTICLE: Genetic inference and single cell expression analysis of potential targets in heart failure and breast cancer
Abstract Background Breast cancer and heart failure are major public health issues globally, characterized by significant genetic heterogeneity and complex molecular mechanisms. This study aims to explore potential genetic targets in heart failure and breast cancer through combining genetic inferenc...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer
2024-10-01
|
| Series: | Journal of Cancer Research and Clinical Oncology |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/s00432-024-06010-y |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850190950386630656 |
|---|---|
| author | Yue Li Ying Huang Ning An Xiaomiao Guan Bing Liu Huiying Li Tingting Jiang |
| author_facet | Yue Li Ying Huang Ning An Xiaomiao Guan Bing Liu Huiying Li Tingting Jiang |
| author_sort | Yue Li |
| collection | DOAJ |
| description | Abstract Background Breast cancer and heart failure are major public health issues globally, characterized by significant genetic heterogeneity and complex molecular mechanisms. This study aims to explore potential genetic targets in heart failure and breast cancer through combining genetic inference and single-cell expression analysis, and to assess the causal relationships of these targets using Mendelian randomization methods. Methods We first identified genetic variants associated with heart failure and breast cancer using genome-wide association study (GWAS) data. Subsequently, we analyzed the expression patterns of these genetic variants in different cell types through single-cell RNA sequencing technology. Furthermore, we utilized in vitro experiment to assess the effects of ITM2B on MCF7 using immunofluorescence. Results Our study identified multiple genetic variants associated with heart failure and breast cancer, showing specific expression patterns in heart and breast cells. The ITM2B gene is highly expressed in breast cancer. ITM2B shows the highest expression in T cells and the lowest expression in proliferating cells. Silencing the ITM2B gene can promote apoptosis and inhibit proliferation and migration of MCF7 breast cancer cells. Conclusion By integrating genetic inference and single-cell expression analysis, this study revealed potential genetic targets in heart failure and breast cancer, and validated the causal effects of these targets using Mendelian randomization methods. |
| format | Article |
| id | doaj-art-59e9328eea5340e5b01521c4ae041fb2 |
| institution | OA Journals |
| issn | 1432-1335 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Springer |
| record_format | Article |
| series | Journal of Cancer Research and Clinical Oncology |
| spelling | doaj-art-59e9328eea5340e5b01521c4ae041fb22025-08-20T02:15:06ZengSpringerJournal of Cancer Research and Clinical Oncology1432-13352024-10-011501011610.1007/s00432-024-06010-yRETRACTED ARTICLE: Genetic inference and single cell expression analysis of potential targets in heart failure and breast cancerYue Li0Ying Huang1Ning An2Xiaomiao Guan3Bing Liu4Huiying Li5Tingting Jiang6Department of Cardiovascular, Liaoning Jinqiu HospitalDepartment of Cardiovascular, Liaoning Jinqiu HospitalDepartment of Cardiovascular, Liaoning Jinqiu HospitalDepartment of Nursing, Shengjing Hospital of China Medical UniversityDepartment of Nursing, Shengjing Hospital of China Medical UniversityDepartment of Nursing, Shengjing Hospital of China Medical UniversityDepartment of Nursing, Shengjing Hospital of China Medical UniversityAbstract Background Breast cancer and heart failure are major public health issues globally, characterized by significant genetic heterogeneity and complex molecular mechanisms. This study aims to explore potential genetic targets in heart failure and breast cancer through combining genetic inference and single-cell expression analysis, and to assess the causal relationships of these targets using Mendelian randomization methods. Methods We first identified genetic variants associated with heart failure and breast cancer using genome-wide association study (GWAS) data. Subsequently, we analyzed the expression patterns of these genetic variants in different cell types through single-cell RNA sequencing technology. Furthermore, we utilized in vitro experiment to assess the effects of ITM2B on MCF7 using immunofluorescence. Results Our study identified multiple genetic variants associated with heart failure and breast cancer, showing specific expression patterns in heart and breast cells. The ITM2B gene is highly expressed in breast cancer. ITM2B shows the highest expression in T cells and the lowest expression in proliferating cells. Silencing the ITM2B gene can promote apoptosis and inhibit proliferation and migration of MCF7 breast cancer cells. Conclusion By integrating genetic inference and single-cell expression analysis, this study revealed potential genetic targets in heart failure and breast cancer, and validated the causal effects of these targets using Mendelian randomization methods.https://doi.org/10.1007/s00432-024-06010-yGenetic inferenceSingle cell expression analysisHeart failureBreast cancerPotential target |
| spellingShingle | Yue Li Ying Huang Ning An Xiaomiao Guan Bing Liu Huiying Li Tingting Jiang RETRACTED ARTICLE: Genetic inference and single cell expression analysis of potential targets in heart failure and breast cancer Journal of Cancer Research and Clinical Oncology Genetic inference Single cell expression analysis Heart failure Breast cancer Potential target |
| title | RETRACTED ARTICLE: Genetic inference and single cell expression analysis of potential targets in heart failure and breast cancer |
| title_full | RETRACTED ARTICLE: Genetic inference and single cell expression analysis of potential targets in heart failure and breast cancer |
| title_fullStr | RETRACTED ARTICLE: Genetic inference and single cell expression analysis of potential targets in heart failure and breast cancer |
| title_full_unstemmed | RETRACTED ARTICLE: Genetic inference and single cell expression analysis of potential targets in heart failure and breast cancer |
| title_short | RETRACTED ARTICLE: Genetic inference and single cell expression analysis of potential targets in heart failure and breast cancer |
| title_sort | retracted article genetic inference and single cell expression analysis of potential targets in heart failure and breast cancer |
| topic | Genetic inference Single cell expression analysis Heart failure Breast cancer Potential target |
| url | https://doi.org/10.1007/s00432-024-06010-y |
| work_keys_str_mv | AT yueli retractedarticlegeneticinferenceandsinglecellexpressionanalysisofpotentialtargetsinheartfailureandbreastcancer AT yinghuang retractedarticlegeneticinferenceandsinglecellexpressionanalysisofpotentialtargetsinheartfailureandbreastcancer AT ningan retractedarticlegeneticinferenceandsinglecellexpressionanalysisofpotentialtargetsinheartfailureandbreastcancer AT xiaomiaoguan retractedarticlegeneticinferenceandsinglecellexpressionanalysisofpotentialtargetsinheartfailureandbreastcancer AT bingliu retractedarticlegeneticinferenceandsinglecellexpressionanalysisofpotentialtargetsinheartfailureandbreastcancer AT huiyingli retractedarticlegeneticinferenceandsinglecellexpressionanalysisofpotentialtargetsinheartfailureandbreastcancer AT tingtingjiang retractedarticlegeneticinferenceandsinglecellexpressionanalysisofpotentialtargetsinheartfailureandbreastcancer |