Effect of cyclosporin A on inflammatory cytokine production by U937 monocyte-like cells
Cyclosporin A (CsA) is an immunosuppresor drug that has been used in the treatment of several types of inflammatory diseases. In some of them the inhibition of T-lymphocyte activation does not suitably account for the observed beneficial effect, suggesting that CsA could act on other types of cells....
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2000-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1080/09629350020008682 |
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| author | Juan E. Losa Garcia María Rosa Martín de Cabo Fernando Mateos Rodríguez Jesús Pérez Losada Antonio Jiménez López José Luis Pérez Arellano |
| author_facet | Juan E. Losa Garcia María Rosa Martín de Cabo Fernando Mateos Rodríguez Jesús Pérez Losada Antonio Jiménez López José Luis Pérez Arellano |
| author_sort | Juan E. Losa Garcia |
| collection | DOAJ |
| description | Cyclosporin A (CsA) is an immunosuppresor drug that has been used in the treatment of several types of inflammatory diseases. In some of them the inhibition of T-lymphocyte activation does not suitably account for the observed beneficial effect, suggesting that CsA could act on other types of cells. The present study was undertaken to determine the effect of CsA on inflammatory cytokine secretion by U937 monocyte cells. Undifferentiated and dimethylsulfoxide (DMSO) differentiated U937 cells were incubated with different concentrations of CsA (200, 20 and 2 ng/mL) in the presence or absence of phorbol-myristateacetate (PMA). Interleukin-1g (IL-1β), tumor necrosis factor-α (TNF-α), IL-6 and IL-8 levels were measured in supernatants using specific enzyme-linked immunosorbent assays. At the highest concentration used (200 ng/mL) CsA decreased the basal and stimulated secretion of all the inflammatory cytokines studied in both undifferentiated and differentiated cells, with the only exception of PMA-stim ulated IL-1 secretion by undifferentiated cells. However, only basal secretion of interleukin-8 in both undifferentiated and DMSO-differentiated U937 cells was significantly reduced by CsA at the highest concentration (200 ng/ mL). At therapeutic concentrations in vivo, CsA exerts a predominant effect on IL-8 secretion by human mononuclear phagocytes. |
| format | Article |
| id | doaj-art-59e9192fdf1a42ee96d200920fe88121 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2000-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-59e9192fdf1a42ee96d200920fe881212025-02-03T01:23:24ZengWileyMediators of Inflammation0962-93511466-18612000-01-0193-416917410.1080/09629350020008682Effect of cyclosporin A on inflammatory cytokine production by U937 monocyte-like cellsJuan E. Losa Garcia0María Rosa Martín de Cabo1Fernando Mateos Rodríguez2Jesús Pérez Losada3Antonio Jiménez López4José Luis Pérez Arellano5Fundación Hospital de Alcorcón, Alcorcón, SpainCentro de Salud Miguel Servet, Alcorcón, SpainHospital General de Albacete, Albacete, SpainUniversidad de Salamanca, Salamanca, SpainUniversidad de Salamanca, Salamanca, SpainDepartamento de Ciencias Clínicas, Universidad de Las Palmas, Las Palmas, SpainCyclosporin A (CsA) is an immunosuppresor drug that has been used in the treatment of several types of inflammatory diseases. In some of them the inhibition of T-lymphocyte activation does not suitably account for the observed beneficial effect, suggesting that CsA could act on other types of cells. The present study was undertaken to determine the effect of CsA on inflammatory cytokine secretion by U937 monocyte cells. Undifferentiated and dimethylsulfoxide (DMSO) differentiated U937 cells were incubated with different concentrations of CsA (200, 20 and 2 ng/mL) in the presence or absence of phorbol-myristateacetate (PMA). Interleukin-1g (IL-1β), tumor necrosis factor-α (TNF-α), IL-6 and IL-8 levels were measured in supernatants using specific enzyme-linked immunosorbent assays. At the highest concentration used (200 ng/mL) CsA decreased the basal and stimulated secretion of all the inflammatory cytokines studied in both undifferentiated and differentiated cells, with the only exception of PMA-stim ulated IL-1 secretion by undifferentiated cells. However, only basal secretion of interleukin-8 in both undifferentiated and DMSO-differentiated U937 cells was significantly reduced by CsA at the highest concentration (200 ng/ mL). At therapeutic concentrations in vivo, CsA exerts a predominant effect on IL-8 secretion by human mononuclear phagocytes.http://dx.doi.org/10.1080/09629350020008682 |
| spellingShingle | Juan E. Losa Garcia María Rosa Martín de Cabo Fernando Mateos Rodríguez Jesús Pérez Losada Antonio Jiménez López José Luis Pérez Arellano Effect of cyclosporin A on inflammatory cytokine production by U937 monocyte-like cells Mediators of Inflammation |
| title | Effect of cyclosporin A on inflammatory cytokine production by U937 monocyte-like cells |
| title_full | Effect of cyclosporin A on inflammatory cytokine production by U937 monocyte-like cells |
| title_fullStr | Effect of cyclosporin A on inflammatory cytokine production by U937 monocyte-like cells |
| title_full_unstemmed | Effect of cyclosporin A on inflammatory cytokine production by U937 monocyte-like cells |
| title_short | Effect of cyclosporin A on inflammatory cytokine production by U937 monocyte-like cells |
| title_sort | effect of cyclosporin a on inflammatory cytokine production by u937 monocyte like cells |
| url | http://dx.doi.org/10.1080/09629350020008682 |
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