Protective and immunomodulatory effects of the novel probiotic yeast Pichia kudriavzevii isolated from a home-made kefir during infection in human colon epithelial cells: An exploratory study
In this study we aimed to explore the protective effects of a probiotic yeast Pichia kudriavzevii (Y1) and its cell-free supernatant (CFS-Y1) during infection simulated in vitro using Caco-2 cells. Results showed that both live P. kudriavzevii and CFS-Y1 effectively decreased Salmonella spp. adhesio...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-02-01
|
| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464625000088 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823864399297773568 |
|---|---|
| author | Angela Maione Marianna Imparato Annalisa Buonanno Marilena Galdiero Elisabetta de Alteriis Marco Guida Emilia Galdiero |
| author_facet | Angela Maione Marianna Imparato Annalisa Buonanno Marilena Galdiero Elisabetta de Alteriis Marco Guida Emilia Galdiero |
| author_sort | Angela Maione |
| collection | DOAJ |
| description | In this study we aimed to explore the protective effects of a probiotic yeast Pichia kudriavzevii (Y1) and its cell-free supernatant (CFS-Y1) during infection simulated in vitro using Caco-2 cells. Results showed that both live P. kudriavzevii and CFS-Y1 effectively decreased Salmonella spp. adhesion and invasion, in competition, inhibition and displacement assays, displaying the most potent inhibitory effects when Caco-2 cells were preincubated with Y1/ CFS-Y1 at different concentrations and then bacteria were added for an additional time. Furthermore, the treatment of LPS-induced Caco-2 cells with both Y1 and its CFS-Y1 effectively relieved the oxidative stress and improved the intestinal TJ barrier function. In particular, Y1 and CFS-Y-1 prevented damage to the intestinal epithelial function in LPS-induced Caco-2 cells by upregulating TJ protein expression and reducing ROS accumulation, as well as overproducing anti-inflammatory cytokines. Therefore, the Y1 and its CFS-Y1 possess potential effects on the maintenance of human health and could be considered as a dietary supplementation for food and a promising approach to treat intestinal barrier dysfunction. |
| format | Article |
| id | doaj-art-59e76739e9714e90a4c5288e82f4be73 |
| institution | Kabale University |
| issn | 1756-4646 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj-art-59e76739e9714e90a4c5288e82f4be732025-02-09T04:59:54ZengElsevierJournal of Functional Foods1756-46462025-02-01125106666Protective and immunomodulatory effects of the novel probiotic yeast Pichia kudriavzevii isolated from a home-made kefir during infection in human colon epithelial cells: An exploratory studyAngela Maione0Marianna Imparato1Annalisa Buonanno2Marilena Galdiero3Elisabetta de Alteriis4Marco Guida5Emilia Galdiero6Department of Biology, University of Naples “Federico II”, 80126 Naples, ItalyDepartment of Biology, University of Naples “Federico II”, 80126 Naples, ItalyDepartment of Biology, University of Naples “Federico II”, 80126 Naples, ItalyDepartment of Experimental Medicine, University of Campania “Luigi Vanvitelli”, 81100 Naples, ItalyDepartment of Biology, University of Naples “Federico II”, 80126 Naples, ItalyDepartment of Biology, University of Naples “Federico II”, 80126 Naples, Italy; BAT Center-Interuniversity Center for Studies on Bioinspired Agro-Environmental Technology, University of Naples Federico II, 80055 Portici, Italy; NBFC, National Biodiversity Future Center, Palermo 90133, ItalyDepartment of Biology, University of Naples “Federico II”, 80126 Naples, Italy; Corresponding author.In this study we aimed to explore the protective effects of a probiotic yeast Pichia kudriavzevii (Y1) and its cell-free supernatant (CFS-Y1) during infection simulated in vitro using Caco-2 cells. Results showed that both live P. kudriavzevii and CFS-Y1 effectively decreased Salmonella spp. adhesion and invasion, in competition, inhibition and displacement assays, displaying the most potent inhibitory effects when Caco-2 cells were preincubated with Y1/ CFS-Y1 at different concentrations and then bacteria were added for an additional time. Furthermore, the treatment of LPS-induced Caco-2 cells with both Y1 and its CFS-Y1 effectively relieved the oxidative stress and improved the intestinal TJ barrier function. In particular, Y1 and CFS-Y-1 prevented damage to the intestinal epithelial function in LPS-induced Caco-2 cells by upregulating TJ protein expression and reducing ROS accumulation, as well as overproducing anti-inflammatory cytokines. Therefore, the Y1 and its CFS-Y1 possess potential effects on the maintenance of human health and could be considered as a dietary supplementation for food and a promising approach to treat intestinal barrier dysfunction.http://www.sciencedirect.com/science/article/pii/S1756464625000088Pichia kudriavzeviiIntestinal barrierAnti-inflammatoryOxidative stressCaco-2 cellsProbiotics |
| spellingShingle | Angela Maione Marianna Imparato Annalisa Buonanno Marilena Galdiero Elisabetta de Alteriis Marco Guida Emilia Galdiero Protective and immunomodulatory effects of the novel probiotic yeast Pichia kudriavzevii isolated from a home-made kefir during infection in human colon epithelial cells: An exploratory study Journal of Functional Foods Pichia kudriavzevii Intestinal barrier Anti-inflammatory Oxidative stress Caco-2 cells Probiotics |
| title | Protective and immunomodulatory effects of the novel probiotic yeast Pichia kudriavzevii isolated from a home-made kefir during infection in human colon epithelial cells: An exploratory study |
| title_full | Protective and immunomodulatory effects of the novel probiotic yeast Pichia kudriavzevii isolated from a home-made kefir during infection in human colon epithelial cells: An exploratory study |
| title_fullStr | Protective and immunomodulatory effects of the novel probiotic yeast Pichia kudriavzevii isolated from a home-made kefir during infection in human colon epithelial cells: An exploratory study |
| title_full_unstemmed | Protective and immunomodulatory effects of the novel probiotic yeast Pichia kudriavzevii isolated from a home-made kefir during infection in human colon epithelial cells: An exploratory study |
| title_short | Protective and immunomodulatory effects of the novel probiotic yeast Pichia kudriavzevii isolated from a home-made kefir during infection in human colon epithelial cells: An exploratory study |
| title_sort | protective and immunomodulatory effects of the novel probiotic yeast pichia kudriavzevii isolated from a home made kefir during infection in human colon epithelial cells an exploratory study |
| topic | Pichia kudriavzevii Intestinal barrier Anti-inflammatory Oxidative stress Caco-2 cells Probiotics |
| url | http://www.sciencedirect.com/science/article/pii/S1756464625000088 |
| work_keys_str_mv | AT angelamaione protectiveandimmunomodulatoryeffectsofthenovelprobioticyeastpichiakudriavzeviiisolatedfromahomemadekefirduringinfectioninhumancolonepithelialcellsanexploratorystudy AT mariannaimparato protectiveandimmunomodulatoryeffectsofthenovelprobioticyeastpichiakudriavzeviiisolatedfromahomemadekefirduringinfectioninhumancolonepithelialcellsanexploratorystudy AT annalisabuonanno protectiveandimmunomodulatoryeffectsofthenovelprobioticyeastpichiakudriavzeviiisolatedfromahomemadekefirduringinfectioninhumancolonepithelialcellsanexploratorystudy AT marilenagaldiero protectiveandimmunomodulatoryeffectsofthenovelprobioticyeastpichiakudriavzeviiisolatedfromahomemadekefirduringinfectioninhumancolonepithelialcellsanexploratorystudy AT elisabettadealteriis protectiveandimmunomodulatoryeffectsofthenovelprobioticyeastpichiakudriavzeviiisolatedfromahomemadekefirduringinfectioninhumancolonepithelialcellsanexploratorystudy AT marcoguida protectiveandimmunomodulatoryeffectsofthenovelprobioticyeastpichiakudriavzeviiisolatedfromahomemadekefirduringinfectioninhumancolonepithelialcellsanexploratorystudy AT emiliagaldiero protectiveandimmunomodulatoryeffectsofthenovelprobioticyeastpichiakudriavzeviiisolatedfromahomemadekefirduringinfectioninhumancolonepithelialcellsanexploratorystudy |