Cell-derived nanovesicles prepared by membrane extrusion are good substitutes for natural extracellular vesicles
Extracellular vesicles (EV) as drug delivery nanocarriers are under intense investigation. Although clinical-grade EVs have been produced on a large-scale, low yield and high production costs of natural EVs (nEV) limit the relevant industrial translation. Recent studies show that mechanical extrusio...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Extracellular Vesicle |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2773041722000026 |
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| author | Yi Wen Qin Fu Ashley Soliwoda Sheng Zhang Mingfeng Zheng Wenjun Mao Yuan Wan |
| author_facet | Yi Wen Qin Fu Ashley Soliwoda Sheng Zhang Mingfeng Zheng Wenjun Mao Yuan Wan |
| author_sort | Yi Wen |
| collection | DOAJ |
| description | Extracellular vesicles (EV) as drug delivery nanocarriers are under intense investigation. Although clinical-grade EVs have been produced on a large-scale, low yield and high production costs of natural EVs (nEV) limit the relevant industrial translation. Recent studies show that mechanical extrusion of cells can generate nEV-like cell-derived nanovesicles (CNV) which can also be used as drug nanocarriers. Moreover, in comparison with nEVs, CNVs have similar physicochemical properties. Nevertheless, a comprehensive comparison of cargo between nEVs and CNVs has not been investigated yet. Therefore, the aim of this study is to profile and compare CNVs to nEVs. Our results show that no significant difference was found in size, morphology, and classical markers between nEVs and CNVs derived from MDA-MB-231 cells. Protein sequencing data reveals the similarity of membrane proteins between the two groups was ∼71%, while it was ∼21% when pertaining to total protein cargo. Notably, a high similarity of membrane proteins was also found between nEVs and CNVs derived from eight additional cancer cell lines. Moreover, analysis of the top 1000 small RNAs with RNA sequencing showed a ∼65% similarity between the two groups. Altogether, we infer from the high similarity of membrane proteins and small RNA cargo that CNVs can be a good substitute for nEVs. In brief, our findings support previous studies with a notion that CNVs yield comparable performance with nEVs and could pave the way for clinical implementation of CNV-based therapeutics in the future. |
| format | Article |
| id | doaj-art-59e14bd35b9649be94d972f353b001cb |
| institution | DOAJ |
| issn | 2773-0417 |
| language | English |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Extracellular Vesicle |
| spelling | doaj-art-59e14bd35b9649be94d972f353b001cb2025-08-20T02:50:23ZengElsevierExtracellular Vesicle2773-04172022-12-01110000410.1016/j.vesic.2022.100004Cell-derived nanovesicles prepared by membrane extrusion are good substitutes for natural extracellular vesiclesYi Wen0Qin Fu1Ashley Soliwoda2Sheng Zhang3Mingfeng Zheng4Wenjun Mao5Yuan Wan6The Pq Laboratory of BiomeDx/Rx, Department of Biomedical Engineering, Binghamton University, Binghamton, NY 13902, USAProteomics & Metabolomics Facility, Cornell Institute of Biotechnology, Cornell University, Ithaca, NY 14853, USAThe Pq Laboratory of BiomeDx/Rx, Department of Biomedical Engineering, Binghamton University, Binghamton, NY 13902, USAProteomics & Metabolomics Facility, Cornell Institute of Biotechnology, Cornell University, Ithaca, NY 14853, USADepartment of Cardiothoracic Surgery, The Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi Jiangsu 214023, ChinaDepartment of Cardiothoracic Surgery, The Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi Jiangsu 214023, China; Correspondence to: 299 Qingyang Road, Wuxi, Jiangsu 214023, China.The Pq Laboratory of BiomeDx/Rx, Department of Biomedical Engineering, Binghamton University, Binghamton, NY 13902, USA; Correspondence to: 65 Murray Hill Road, Biotechnology Building BI2625, Binghamton University, Vestal, NY 13850, USA.Extracellular vesicles (EV) as drug delivery nanocarriers are under intense investigation. Although clinical-grade EVs have been produced on a large-scale, low yield and high production costs of natural EVs (nEV) limit the relevant industrial translation. Recent studies show that mechanical extrusion of cells can generate nEV-like cell-derived nanovesicles (CNV) which can also be used as drug nanocarriers. Moreover, in comparison with nEVs, CNVs have similar physicochemical properties. Nevertheless, a comprehensive comparison of cargo between nEVs and CNVs has not been investigated yet. Therefore, the aim of this study is to profile and compare CNVs to nEVs. Our results show that no significant difference was found in size, morphology, and classical markers between nEVs and CNVs derived from MDA-MB-231 cells. Protein sequencing data reveals the similarity of membrane proteins between the two groups was ∼71%, while it was ∼21% when pertaining to total protein cargo. Notably, a high similarity of membrane proteins was also found between nEVs and CNVs derived from eight additional cancer cell lines. Moreover, analysis of the top 1000 small RNAs with RNA sequencing showed a ∼65% similarity between the two groups. Altogether, we infer from the high similarity of membrane proteins and small RNA cargo that CNVs can be a good substitute for nEVs. In brief, our findings support previous studies with a notion that CNVs yield comparable performance with nEVs and could pave the way for clinical implementation of CNV-based therapeutics in the future.http://www.sciencedirect.com/science/article/pii/S2773041722000026Cell engineered vesiclesExtracellular vesiclesNext-generation sequencingMass spectrometryDrug delivery |
| spellingShingle | Yi Wen Qin Fu Ashley Soliwoda Sheng Zhang Mingfeng Zheng Wenjun Mao Yuan Wan Cell-derived nanovesicles prepared by membrane extrusion are good substitutes for natural extracellular vesicles Extracellular Vesicle Cell engineered vesicles Extracellular vesicles Next-generation sequencing Mass spectrometry Drug delivery |
| title | Cell-derived nanovesicles prepared by membrane extrusion are good substitutes for natural extracellular vesicles |
| title_full | Cell-derived nanovesicles prepared by membrane extrusion are good substitutes for natural extracellular vesicles |
| title_fullStr | Cell-derived nanovesicles prepared by membrane extrusion are good substitutes for natural extracellular vesicles |
| title_full_unstemmed | Cell-derived nanovesicles prepared by membrane extrusion are good substitutes for natural extracellular vesicles |
| title_short | Cell-derived nanovesicles prepared by membrane extrusion are good substitutes for natural extracellular vesicles |
| title_sort | cell derived nanovesicles prepared by membrane extrusion are good substitutes for natural extracellular vesicles |
| topic | Cell engineered vesicles Extracellular vesicles Next-generation sequencing Mass spectrometry Drug delivery |
| url | http://www.sciencedirect.com/science/article/pii/S2773041722000026 |
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