Umbilical cord mesenchymal stem cells improve bone regeneration in diabetes mellitus animal model with apical periodontitis

Background: Previous studies revealed diabetes mellitus subjects tend to have persistent apical periodontitis. Regenerative stem cells therapy through endodontic procedure is hoped to be a solution. This study assessed bone regeneration in diabetic rats with apical periodontitis through histopatholo...

Full description

Saved in:
Bibliographic Details
Main Authors: Eric Priyo Prasetyo, Pravinna Saravanan, Deaniddo Kharisna, Christina Immee Wijanarko, Mefina Kuntjoro, Nike Hendrijantini, Evelyn Tjendronegoro
Format: Article
Language:English
Published: Elsevier 2025-01-01
Series:Journal of Oral Biology and Craniofacial Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2212426824001763
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1823864399012560896
author Eric Priyo Prasetyo
Pravinna Saravanan
Deaniddo Kharisna
Christina Immee Wijanarko
Mefina Kuntjoro
Nike Hendrijantini
Evelyn Tjendronegoro
author_facet Eric Priyo Prasetyo
Pravinna Saravanan
Deaniddo Kharisna
Christina Immee Wijanarko
Mefina Kuntjoro
Nike Hendrijantini
Evelyn Tjendronegoro
author_sort Eric Priyo Prasetyo
collection DOAJ
description Background: Previous studies revealed diabetes mellitus subjects tend to have persistent apical periodontitis. Regenerative stem cells therapy through endodontic procedure is hoped to be a solution. This study assessed bone regeneration in diabetic rats with apical periodontitis through histopathological analysis of osteoblasts and immunohistochemical analysis of runt-related transcription factor 2 (Runx2) and Osterix. Methods: Diabetes mellitus and apical periodontitis was induced on 20 rats. Apical periodontitis was induced on mandibular right first molars under anesthesia. The teeth were left open for 7 days following access cavity and pulp extirpation, then the rats’ teeth were endodontically treated and randomly allocated into 4 groups (5 rats per group). The first and second groups was ended at 30 days (C30) and 60 days (C60) and labelled as control. The third and fourth groups was given umbilical cord mesenchymal stem cells and ended at 30 days (T30) and 60 days (T60). The osteoblasts, Runx2 and Osterix were analyzed. ANOVA and Tukey HSD tests were used for analysis. Differences with p values < 0.05 were considered significant. Results: The number of osteoblasts in the apical area in control groups (C30 and C60) and treatment groups (T30 and T60) showed a significant increase (p < 0.05). The expressions of Runx2 and Osterix in osteoblasts showed a significant increase among the control (C30 and C60) and treatment groups (T30 and T60) (p < 0.05). Conclusion: Umbilical cord mesenchymal stem cells improve bone regeneration in diabetic animal model with apical periodontitis, in terms of osteoblasts, Runx2 and Osterix.
format Article
id doaj-art-59dffafefe79469d949173153821d72d
institution Kabale University
issn 2212-4268
language English
publishDate 2025-01-01
publisher Elsevier
record_format Article
series Journal of Oral Biology and Craniofacial Research
spelling doaj-art-59dffafefe79469d949173153821d72d2025-02-09T05:00:09ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682025-01-011511621Umbilical cord mesenchymal stem cells improve bone regeneration in diabetes mellitus animal model with apical periodontitisEric Priyo Prasetyo0Pravinna Saravanan1Deaniddo Kharisna2Christina Immee Wijanarko3Mefina Kuntjoro4Nike Hendrijantini5Evelyn Tjendronegoro6Department of Conservative Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia; Corresponding author. Department of Conservative Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Jalan Mayjen Prof Dr Moestopo 47, Surabaya, 60132, Indonesia.Department of Conservative Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaDepartment of Conservative Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaDepartment of Conservative Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaDepartment of Prosthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaDepartment of Prosthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, IndonesiaHealthcare and Research, Irvine Medical Center, University of California, Irvine, United StatesBackground: Previous studies revealed diabetes mellitus subjects tend to have persistent apical periodontitis. Regenerative stem cells therapy through endodontic procedure is hoped to be a solution. This study assessed bone regeneration in diabetic rats with apical periodontitis through histopathological analysis of osteoblasts and immunohistochemical analysis of runt-related transcription factor 2 (Runx2) and Osterix. Methods: Diabetes mellitus and apical periodontitis was induced on 20 rats. Apical periodontitis was induced on mandibular right first molars under anesthesia. The teeth were left open for 7 days following access cavity and pulp extirpation, then the rats’ teeth were endodontically treated and randomly allocated into 4 groups (5 rats per group). The first and second groups was ended at 30 days (C30) and 60 days (C60) and labelled as control. The third and fourth groups was given umbilical cord mesenchymal stem cells and ended at 30 days (T30) and 60 days (T60). The osteoblasts, Runx2 and Osterix were analyzed. ANOVA and Tukey HSD tests were used for analysis. Differences with p values < 0.05 were considered significant. Results: The number of osteoblasts in the apical area in control groups (C30 and C60) and treatment groups (T30 and T60) showed a significant increase (p < 0.05). The expressions of Runx2 and Osterix in osteoblasts showed a significant increase among the control (C30 and C60) and treatment groups (T30 and T60) (p < 0.05). Conclusion: Umbilical cord mesenchymal stem cells improve bone regeneration in diabetic animal model with apical periodontitis, in terms of osteoblasts, Runx2 and Osterix.http://www.sciencedirect.com/science/article/pii/S2212426824001763Apical periodontitisBone regenerationDiabetes mellitusEndodontic treatmentMesenchymal stem cells
spellingShingle Eric Priyo Prasetyo
Pravinna Saravanan
Deaniddo Kharisna
Christina Immee Wijanarko
Mefina Kuntjoro
Nike Hendrijantini
Evelyn Tjendronegoro
Umbilical cord mesenchymal stem cells improve bone regeneration in diabetes mellitus animal model with apical periodontitis
Journal of Oral Biology and Craniofacial Research
Apical periodontitis
Bone regeneration
Diabetes mellitus
Endodontic treatment
Mesenchymal stem cells
title Umbilical cord mesenchymal stem cells improve bone regeneration in diabetes mellitus animal model with apical periodontitis
title_full Umbilical cord mesenchymal stem cells improve bone regeneration in diabetes mellitus animal model with apical periodontitis
title_fullStr Umbilical cord mesenchymal stem cells improve bone regeneration in diabetes mellitus animal model with apical periodontitis
title_full_unstemmed Umbilical cord mesenchymal stem cells improve bone regeneration in diabetes mellitus animal model with apical periodontitis
title_short Umbilical cord mesenchymal stem cells improve bone regeneration in diabetes mellitus animal model with apical periodontitis
title_sort umbilical cord mesenchymal stem cells improve bone regeneration in diabetes mellitus animal model with apical periodontitis
topic Apical periodontitis
Bone regeneration
Diabetes mellitus
Endodontic treatment
Mesenchymal stem cells
url http://www.sciencedirect.com/science/article/pii/S2212426824001763
work_keys_str_mv AT ericpriyoprasetyo umbilicalcordmesenchymalstemcellsimproveboneregenerationindiabetesmellitusanimalmodelwithapicalperiodontitis
AT pravinnasaravanan umbilicalcordmesenchymalstemcellsimproveboneregenerationindiabetesmellitusanimalmodelwithapicalperiodontitis
AT deaniddokharisna umbilicalcordmesenchymalstemcellsimproveboneregenerationindiabetesmellitusanimalmodelwithapicalperiodontitis
AT christinaimmeewijanarko umbilicalcordmesenchymalstemcellsimproveboneregenerationindiabetesmellitusanimalmodelwithapicalperiodontitis
AT mefinakuntjoro umbilicalcordmesenchymalstemcellsimproveboneregenerationindiabetesmellitusanimalmodelwithapicalperiodontitis
AT nikehendrijantini umbilicalcordmesenchymalstemcellsimproveboneregenerationindiabetesmellitusanimalmodelwithapicalperiodontitis
AT evelyntjendronegoro umbilicalcordmesenchymalstemcellsimproveboneregenerationindiabetesmellitusanimalmodelwithapicalperiodontitis